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Absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease
BACKGROUND: Alcoholic liver disease (ALD) is one of the most prevalent chronic liver disease worldwide. Alcohol-induced alterations in hepatic lipids play an important role in ALD develpoment and progression. The present study aimed to thoroughly describe the changes of lipid profiling in liver of m...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254412/ https://www.ncbi.nlm.nih.gov/pubmed/35790996 http://dx.doi.org/10.1186/s12986-022-00679-z |
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author | Zhao, Fei Chen, Jun Guo, Rui Zhu, Jinyan Gu, Weijia Li, Songtao Li, Jiaomei |
author_facet | Zhao, Fei Chen, Jun Guo, Rui Zhu, Jinyan Gu, Weijia Li, Songtao Li, Jiaomei |
author_sort | Zhao, Fei |
collection | PubMed |
description | BACKGROUND: Alcoholic liver disease (ALD) is one of the most prevalent chronic liver disease worldwide. Alcohol-induced alterations in hepatic lipids play an important role in ALD develpoment and progression. The present study aimed to thoroughly describe the changes of lipid profiling in liver of mice with early-stage alcoholic liver disease. METHODS: C57BL/6J male mice aged 7-week were randomized into alcohol-fed (AF) group and pair-fed control group (PF) (n = 10 per group). The early stage of ALD was induced with Lieber-DeCarli liquid diet. The lipids profiling was analyzed by absolute quantitative lipidomics with UHPLC-QTRAP-MS/MS. RESULTS: Alcohol intake significantly increased the levels of alanine aminotransferase (ALT) in plasma, and tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and triacylglycerols (TAG) levels in liver. Lipidomis analyses showed that 41 TAGs were up-regulated and 8 TAGs were down-regulated in response to alcohol intake. The 8 decreased TAGs were with more double bond, longer carbon chain length and mostly contained docosahexaenoic acid (C22:6n-3) and eicosapentaenoic acid (C20:5n-3), compared with the up-regulated TAGs. Furthermore, the down-regulated TAG(56:9)_FA20:5 was inversely associated with ALT and IL-6 levels. In addition, several altered lysophosphatidylcholines (LPC), lysophosphatidylethanolamines (LPE) and hexosylceramides (HCER) were all significantly decreased in response to alcohol consumption, especially HCer(18:1/22:0), with the top reduction among all the down-regulated lipids. CONCLUSIONS: These findings suggest that not only the up-regulated lipids, alcohol-induced reduction in some specific lipids might also contribute to the ALD development, especially TAG(56:9)_FA20:5 and HCer(18:1/22:0). Their physiological functions and effects on ALD development warrants further investigation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-022-00679-z. |
format | Online Article Text |
id | pubmed-9254412 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92544122022-07-06 Absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease Zhao, Fei Chen, Jun Guo, Rui Zhu, Jinyan Gu, Weijia Li, Songtao Li, Jiaomei Nutr Metab (Lond) Research BACKGROUND: Alcoholic liver disease (ALD) is one of the most prevalent chronic liver disease worldwide. Alcohol-induced alterations in hepatic lipids play an important role in ALD develpoment and progression. The present study aimed to thoroughly describe the changes of lipid profiling in liver of mice with early-stage alcoholic liver disease. METHODS: C57BL/6J male mice aged 7-week were randomized into alcohol-fed (AF) group and pair-fed control group (PF) (n = 10 per group). The early stage of ALD was induced with Lieber-DeCarli liquid diet. The lipids profiling was analyzed by absolute quantitative lipidomics with UHPLC-QTRAP-MS/MS. RESULTS: Alcohol intake significantly increased the levels of alanine aminotransferase (ALT) in plasma, and tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and triacylglycerols (TAG) levels in liver. Lipidomis analyses showed that 41 TAGs were up-regulated and 8 TAGs were down-regulated in response to alcohol intake. The 8 decreased TAGs were with more double bond, longer carbon chain length and mostly contained docosahexaenoic acid (C22:6n-3) and eicosapentaenoic acid (C20:5n-3), compared with the up-regulated TAGs. Furthermore, the down-regulated TAG(56:9)_FA20:5 was inversely associated with ALT and IL-6 levels. In addition, several altered lysophosphatidylcholines (LPC), lysophosphatidylethanolamines (LPE) and hexosylceramides (HCER) were all significantly decreased in response to alcohol consumption, especially HCer(18:1/22:0), with the top reduction among all the down-regulated lipids. CONCLUSIONS: These findings suggest that not only the up-regulated lipids, alcohol-induced reduction in some specific lipids might also contribute to the ALD development, especially TAG(56:9)_FA20:5 and HCer(18:1/22:0). Their physiological functions and effects on ALD development warrants further investigation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-022-00679-z. BioMed Central 2022-07-05 /pmc/articles/PMC9254412/ /pubmed/35790996 http://dx.doi.org/10.1186/s12986-022-00679-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhao, Fei Chen, Jun Guo, Rui Zhu, Jinyan Gu, Weijia Li, Songtao Li, Jiaomei Absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease |
title | Absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease |
title_full | Absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease |
title_fullStr | Absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease |
title_full_unstemmed | Absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease |
title_short | Absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease |
title_sort | absolute quantitative lipidomics reveals lipids profiling in liver of mice with early-stage alcoholic liver disease |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254412/ https://www.ncbi.nlm.nih.gov/pubmed/35790996 http://dx.doi.org/10.1186/s12986-022-00679-z |
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