New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

• A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq. • Spep-1 showed nanomolar affinity and high specificity to spike protein. • S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples. • Spep-1 can be used in future on S2 recognition, virus...

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Autores principales: Chen, Mengyuan, He, Shuizhen, Xiong, Hualong, Zhang, Dongxu, Wang, Shaojuan, Hou, Wangheng, Zhu, Xiaomei, Wang, Jin, Huang, Yang, Hong, Congming, Wu, Yubin, Qi, Ruoyao, Zhang, Tianying, Yuan, Quan, Li, Tingdong, Chen, Yixin, Zhang, Shiyin, Ge, Shengxiang, Zhang, Jun, Xia, Ningshao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254439/
https://www.ncbi.nlm.nih.gov/pubmed/35803529
http://dx.doi.org/10.1016/j.virs.2022.07.001
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author Chen, Mengyuan
He, Shuizhen
Xiong, Hualong
Zhang, Dongxu
Wang, Shaojuan
Hou, Wangheng
Zhu, Xiaomei
Wang, Jin
Huang, Yang
Hong, Congming
Wu, Yubin
Qi, Ruoyao
Zhang, Tianying
Yuan, Quan
Li, Tingdong
Chen, Yixin
Zhang, Shiyin
Ge, Shengxiang
Zhang, Jun
Xia, Ningshao
author_facet Chen, Mengyuan
He, Shuizhen
Xiong, Hualong
Zhang, Dongxu
Wang, Shaojuan
Hou, Wangheng
Zhu, Xiaomei
Wang, Jin
Huang, Yang
Hong, Congming
Wu, Yubin
Qi, Ruoyao
Zhang, Tianying
Yuan, Quan
Li, Tingdong
Chen, Yixin
Zhang, Shiyin
Ge, Shengxiang
Zhang, Jun
Xia, Ningshao
author_sort Chen, Mengyuan
collection PubMed
description • A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq. • Spep-1 showed nanomolar affinity and high specificity to spike protein. • S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples. • Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery.
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spelling pubmed-92544392022-07-05 New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq Chen, Mengyuan He, Shuizhen Xiong, Hualong Zhang, Dongxu Wang, Shaojuan Hou, Wangheng Zhu, Xiaomei Wang, Jin Huang, Yang Hong, Congming Wu, Yubin Qi, Ruoyao Zhang, Tianying Yuan, Quan Li, Tingdong Chen, Yixin Zhang, Shiyin Ge, Shengxiang Zhang, Jun Xia, Ningshao Virol Sin Letter • A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq. • Spep-1 showed nanomolar affinity and high specificity to spike protein. • S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples. • Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-07-05 /pmc/articles/PMC9254439/ /pubmed/35803529 http://dx.doi.org/10.1016/j.virs.2022.07.001 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Letter
Chen, Mengyuan
He, Shuizhen
Xiong, Hualong
Zhang, Dongxu
Wang, Shaojuan
Hou, Wangheng
Zhu, Xiaomei
Wang, Jin
Huang, Yang
Hong, Congming
Wu, Yubin
Qi, Ruoyao
Zhang, Tianying
Yuan, Quan
Li, Tingdong
Chen, Yixin
Zhang, Shiyin
Ge, Shengxiang
Zhang, Jun
Xia, Ningshao
New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq
title New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq
title_full New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq
title_fullStr New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq
title_full_unstemmed New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq
title_short New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq
title_sort new discovery of high-affinity sars-cov-2 spike s2 protein binding peptide selected by phip-seq
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254439/
https://www.ncbi.nlm.nih.gov/pubmed/35803529
http://dx.doi.org/10.1016/j.virs.2022.07.001
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