Protocol for assessing exvivo lipolysis of murine adipose tissue

Here, we provide a detailed protocol for assessing exvivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays...

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Detalles Bibliográficos
Autores principales: Roy, Debasish, Myers, Julia M., Tedeschi, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254440/
https://www.ncbi.nlm.nih.gov/pubmed/35779261
http://dx.doi.org/10.1016/j.xpro.2022.101518
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author Roy, Debasish
Myers, Julia M.
Tedeschi, Andrea
author_facet Roy, Debasish
Myers, Julia M.
Tedeschi, Andrea
author_sort Roy, Debasish
collection PubMed
description Here, we provide a detailed protocol for assessing exvivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease.
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spelling pubmed-92544402022-07-06 Protocol for assessing exvivo lipolysis of murine adipose tissue Roy, Debasish Myers, Julia M. Tedeschi, Andrea STAR Protoc Protocol Here, we provide a detailed protocol for assessing exvivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease. Elsevier 2022-07-01 /pmc/articles/PMC9254440/ /pubmed/35779261 http://dx.doi.org/10.1016/j.xpro.2022.101518 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Roy, Debasish
Myers, Julia M.
Tedeschi, Andrea
Protocol for assessing exvivo lipolysis of murine adipose tissue
title Protocol for assessing exvivo lipolysis of murine adipose tissue
title_full Protocol for assessing exvivo lipolysis of murine adipose tissue
title_fullStr Protocol for assessing exvivo lipolysis of murine adipose tissue
title_full_unstemmed Protocol for assessing exvivo lipolysis of murine adipose tissue
title_short Protocol for assessing exvivo lipolysis of murine adipose tissue
title_sort protocol for assessing exvivo lipolysis of murine adipose tissue
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254440/
https://www.ncbi.nlm.nih.gov/pubmed/35779261
http://dx.doi.org/10.1016/j.xpro.2022.101518
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