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Quantitative phase imaging through an ultra-thin lensless fiber endoscope

Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such a powerful technique to in vivo pathological diagnosis remains challenging. Multi-core fiber bundles (MCFs) enable ultra-thin probes fo...

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Autores principales: Sun, Jiawei, Wu, Jiachen, Wu, Song, Goswami, Ruchi, Girardo, Salvatore, Cao, Liangcai, Guck, Jochen, Koukourakis, Nektarios, Czarske, Juergen W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9255502/
https://www.ncbi.nlm.nih.gov/pubmed/35790748
http://dx.doi.org/10.1038/s41377-022-00898-2
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author Sun, Jiawei
Wu, Jiachen
Wu, Song
Goswami, Ruchi
Girardo, Salvatore
Cao, Liangcai
Guck, Jochen
Koukourakis, Nektarios
Czarske, Juergen W.
author_facet Sun, Jiawei
Wu, Jiachen
Wu, Song
Goswami, Ruchi
Girardo, Salvatore
Cao, Liangcai
Guck, Jochen
Koukourakis, Nektarios
Czarske, Juergen W.
author_sort Sun, Jiawei
collection PubMed
description Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such a powerful technique to in vivo pathological diagnosis remains challenging. Multi-core fiber bundles (MCFs) enable ultra-thin probes for in vivo imaging, but current MCF imaging techniques are limited to amplitude imaging modalities. We demonstrate a computational lensless microendoscope that uses an ultra-thin bare MCF to perform quantitative phase imaging with microscale lateral resolution and nanoscale axial sensitivity of the optical path length. The incident complex light field at the measurement side is precisely reconstructed from the far-field speckle pattern at the detection side, enabling digital refocusing in a multi-layer sample without any mechanical movement. The accuracy of the quantitative phase reconstruction is validated by imaging the phase target and hydrogel beads through the MCF. With the proposed imaging modality, three-dimensional imaging of human cancer cells is achieved through the ultra-thin fiber endoscope, promising widespread clinical applications.
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spelling pubmed-92555022022-07-06 Quantitative phase imaging through an ultra-thin lensless fiber endoscope Sun, Jiawei Wu, Jiachen Wu, Song Goswami, Ruchi Girardo, Salvatore Cao, Liangcai Guck, Jochen Koukourakis, Nektarios Czarske, Juergen W. Light Sci Appl Article Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such a powerful technique to in vivo pathological diagnosis remains challenging. Multi-core fiber bundles (MCFs) enable ultra-thin probes for in vivo imaging, but current MCF imaging techniques are limited to amplitude imaging modalities. We demonstrate a computational lensless microendoscope that uses an ultra-thin bare MCF to perform quantitative phase imaging with microscale lateral resolution and nanoscale axial sensitivity of the optical path length. The incident complex light field at the measurement side is precisely reconstructed from the far-field speckle pattern at the detection side, enabling digital refocusing in a multi-layer sample without any mechanical movement. The accuracy of the quantitative phase reconstruction is validated by imaging the phase target and hydrogel beads through the MCF. With the proposed imaging modality, three-dimensional imaging of human cancer cells is achieved through the ultra-thin fiber endoscope, promising widespread clinical applications. Nature Publishing Group UK 2022-07-05 /pmc/articles/PMC9255502/ /pubmed/35790748 http://dx.doi.org/10.1038/s41377-022-00898-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sun, Jiawei
Wu, Jiachen
Wu, Song
Goswami, Ruchi
Girardo, Salvatore
Cao, Liangcai
Guck, Jochen
Koukourakis, Nektarios
Czarske, Juergen W.
Quantitative phase imaging through an ultra-thin lensless fiber endoscope
title Quantitative phase imaging through an ultra-thin lensless fiber endoscope
title_full Quantitative phase imaging through an ultra-thin lensless fiber endoscope
title_fullStr Quantitative phase imaging through an ultra-thin lensless fiber endoscope
title_full_unstemmed Quantitative phase imaging through an ultra-thin lensless fiber endoscope
title_short Quantitative phase imaging through an ultra-thin lensless fiber endoscope
title_sort quantitative phase imaging through an ultra-thin lensless fiber endoscope
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9255502/
https://www.ncbi.nlm.nih.gov/pubmed/35790748
http://dx.doi.org/10.1038/s41377-022-00898-2
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