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A simple specimen preparation method for histopathological evaluation of vestibular organs
Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this stud...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Toxicologic Pathology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256003/ https://www.ncbi.nlm.nih.gov/pubmed/35832898 http://dx.doi.org/10.1293/tox.2022-0008 |
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author | Onishi, Shinichi Murai, Atsuko Kito, Aki Kawashima, Yuka Ohmori, Yusuke Kato, Atsuhiko |
author_facet | Onishi, Shinichi Murai, Atsuko Kito, Aki Kawashima, Yuka Ohmori, Yusuke Kato, Atsuhiko |
author_sort | Onishi, Shinichi |
collection | PubMed |
description | Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this study, we investigated highly reproducible methods of preparing vestibular organ specimens for histopathological examinations. We established a method that allows researchers to observe the utricle and saccule, including otoliths, the ampulla of a semicircular duct, and parts of semicircular ducts. This highly reproducible method is useful for histopathological analysis of mice with symptoms of abnormal equilibrium caused by medical toxicity and genetic modification. |
format | Online Article Text |
id | pubmed-9256003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Japanese Society of Toxicologic Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-92560032022-07-12 A simple specimen preparation method for histopathological evaluation of vestibular organs Onishi, Shinichi Murai, Atsuko Kito, Aki Kawashima, Yuka Ohmori, Yusuke Kato, Atsuhiko J Toxicol Pathol Technical Report Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this study, we investigated highly reproducible methods of preparing vestibular organ specimens for histopathological examinations. We established a method that allows researchers to observe the utricle and saccule, including otoliths, the ampulla of a semicircular duct, and parts of semicircular ducts. This highly reproducible method is useful for histopathological analysis of mice with symptoms of abnormal equilibrium caused by medical toxicity and genetic modification. Japanese Society of Toxicologic Pathology 2022-05-07 2022-07 /pmc/articles/PMC9256003/ /pubmed/35832898 http://dx.doi.org/10.1293/tox.2022-0008 Text en ©2022 The Japanese Society of Toxicologic Pathology https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Technical Report Onishi, Shinichi Murai, Atsuko Kito, Aki Kawashima, Yuka Ohmori, Yusuke Kato, Atsuhiko A simple specimen preparation method for histopathological evaluation of vestibular organs |
title | A simple specimen preparation method for histopathological evaluation of
vestibular organs |
title_full | A simple specimen preparation method for histopathological evaluation of
vestibular organs |
title_fullStr | A simple specimen preparation method for histopathological evaluation of
vestibular organs |
title_full_unstemmed | A simple specimen preparation method for histopathological evaluation of
vestibular organs |
title_short | A simple specimen preparation method for histopathological evaluation of
vestibular organs |
title_sort | simple specimen preparation method for histopathological evaluation of
vestibular organs |
topic | Technical Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256003/ https://www.ncbi.nlm.nih.gov/pubmed/35832898 http://dx.doi.org/10.1293/tox.2022-0008 |
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