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A simple specimen preparation method for histopathological evaluation of vestibular organs

Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this stud...

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Detalles Bibliográficos
Autores principales: Onishi, Shinichi, Murai, Atsuko, Kito, Aki, Kawashima, Yuka, Ohmori, Yusuke, Kato, Atsuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Toxicologic Pathology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256003/
https://www.ncbi.nlm.nih.gov/pubmed/35832898
http://dx.doi.org/10.1293/tox.2022-0008
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author Onishi, Shinichi
Murai, Atsuko
Kito, Aki
Kawashima, Yuka
Ohmori, Yusuke
Kato, Atsuhiko
author_facet Onishi, Shinichi
Murai, Atsuko
Kito, Aki
Kawashima, Yuka
Ohmori, Yusuke
Kato, Atsuhiko
author_sort Onishi, Shinichi
collection PubMed
description Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this study, we investigated highly reproducible methods of preparing vestibular organ specimens for histopathological examinations. We established a method that allows researchers to observe the utricle and saccule, including otoliths, the ampulla of a semicircular duct, and parts of semicircular ducts. This highly reproducible method is useful for histopathological analysis of mice with symptoms of abnormal equilibrium caused by medical toxicity and genetic modification.
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spelling pubmed-92560032022-07-12 A simple specimen preparation method for histopathological evaluation of vestibular organs Onishi, Shinichi Murai, Atsuko Kito, Aki Kawashima, Yuka Ohmori, Yusuke Kato, Atsuhiko J Toxicol Pathol Technical Report Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this study, we investigated highly reproducible methods of preparing vestibular organ specimens for histopathological examinations. We established a method that allows researchers to observe the utricle and saccule, including otoliths, the ampulla of a semicircular duct, and parts of semicircular ducts. This highly reproducible method is useful for histopathological analysis of mice with symptoms of abnormal equilibrium caused by medical toxicity and genetic modification. Japanese Society of Toxicologic Pathology 2022-05-07 2022-07 /pmc/articles/PMC9256003/ /pubmed/35832898 http://dx.doi.org/10.1293/tox.2022-0008 Text en ©2022 The Japanese Society of Toxicologic Pathology https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Technical Report
Onishi, Shinichi
Murai, Atsuko
Kito, Aki
Kawashima, Yuka
Ohmori, Yusuke
Kato, Atsuhiko
A simple specimen preparation method for histopathological evaluation of vestibular organs
title A simple specimen preparation method for histopathological evaluation of vestibular organs
title_full A simple specimen preparation method for histopathological evaluation of vestibular organs
title_fullStr A simple specimen preparation method for histopathological evaluation of vestibular organs
title_full_unstemmed A simple specimen preparation method for histopathological evaluation of vestibular organs
title_short A simple specimen preparation method for histopathological evaluation of vestibular organs
title_sort simple specimen preparation method for histopathological evaluation of vestibular organs
topic Technical Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256003/
https://www.ncbi.nlm.nih.gov/pubmed/35832898
http://dx.doi.org/10.1293/tox.2022-0008
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