Cargando…
Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
Kinesin-13 (Kin-13), a depolymerizer of microtubule (MT), has been known to affect the length of Giardia. Giardia Kin-13 (GlKin-13) was localized to axoneme, flagellar tips, and centrosomes, where phosphorylated forms of Giardia polo-like kinase (GlPLK) were distributed. We observed the interaction...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Parasitology and Tropical Medicine
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256289/ https://www.ncbi.nlm.nih.gov/pubmed/35772734 http://dx.doi.org/10.3347/kjp.2022.60.3.163 |
_version_ | 1784741078587408384 |
---|---|
author | Park, Eun-Ah Kim, Juri Shin, Mee Young Park, Soon-Jung |
author_facet | Park, Eun-Ah Kim, Juri Shin, Mee Young Park, Soon-Jung |
author_sort | Park, Eun-Ah |
collection | PubMed |
description | Kinesin-13 (Kin-13), a depolymerizer of microtubule (MT), has been known to affect the length of Giardia. Giardia Kin-13 (GlKin-13) was localized to axoneme, flagellar tips, and centrosomes, where phosphorylated forms of Giardia polo-like kinase (GlPLK) were distributed. We observed the interaction between GlKin-13 and GlPLK via co-immunoprecipitation using transgenic Giardia cells expressing Myc-tagged GlKin-13, hemagglutinin-tagged GlPLK, and in vitro-synthesized GlKin-13 and GlPLK proteins. In vitro-synthesized GlPLK was demonstrated to auto-phosphorylate and phosphorylate GlKin-13 upon incubation with [γ-(32)P]ATP. Morpholino-mediated depletion of both GlKin-13 and GlPLK caused an extension of flagella and a decreased volume of median bodies in Giardia trophozoites. Our results suggest that GlPLK plays a pertinent role in formation of flagella and median bodies by modulating MT depolymerizing activity of GlKin-13. |
format | Online Article Text |
id | pubmed-9256289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Korean Society for Parasitology and Tropical Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-92562892022-07-11 Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia Park, Eun-Ah Kim, Juri Shin, Mee Young Park, Soon-Jung Korean J Parasitol Original Article Kinesin-13 (Kin-13), a depolymerizer of microtubule (MT), has been known to affect the length of Giardia. Giardia Kin-13 (GlKin-13) was localized to axoneme, flagellar tips, and centrosomes, where phosphorylated forms of Giardia polo-like kinase (GlPLK) were distributed. We observed the interaction between GlKin-13 and GlPLK via co-immunoprecipitation using transgenic Giardia cells expressing Myc-tagged GlKin-13, hemagglutinin-tagged GlPLK, and in vitro-synthesized GlKin-13 and GlPLK proteins. In vitro-synthesized GlPLK was demonstrated to auto-phosphorylate and phosphorylate GlKin-13 upon incubation with [γ-(32)P]ATP. Morpholino-mediated depletion of both GlKin-13 and GlPLK caused an extension of flagella and a decreased volume of median bodies in Giardia trophozoites. Our results suggest that GlPLK plays a pertinent role in formation of flagella and median bodies by modulating MT depolymerizing activity of GlKin-13. The Korean Society for Parasitology and Tropical Medicine 2022-06 2022-06-30 /pmc/articles/PMC9256289/ /pubmed/35772734 http://dx.doi.org/10.3347/kjp.2022.60.3.163 Text en © 2022, Korean Society for Parasitology and Tropical Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Park, Eun-Ah Kim, Juri Shin, Mee Young Park, Soon-Jung Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia |
title | Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia |
title_full | Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia |
title_fullStr | Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia |
title_full_unstemmed | Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia |
title_short | Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia |
title_sort | kinesin-13, a motor protein, is regulated by polo-like kinase in giardia lamblia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256289/ https://www.ncbi.nlm.nih.gov/pubmed/35772734 http://dx.doi.org/10.3347/kjp.2022.60.3.163 |
work_keys_str_mv | AT parkeunah kinesin13amotorproteinisregulatedbypololikekinaseingiardialamblia AT kimjuri kinesin13amotorproteinisregulatedbypololikekinaseingiardialamblia AT shinmeeyoung kinesin13amotorproteinisregulatedbypololikekinaseingiardialamblia AT parksoonjung kinesin13amotorproteinisregulatedbypololikekinaseingiardialamblia |