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Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia

Kinesin-13 (Kin-13), a depolymerizer of microtubule (MT), has been known to affect the length of Giardia. Giardia Kin-13 (GlKin-13) was localized to axoneme, flagellar tips, and centrosomes, where phosphorylated forms of Giardia polo-like kinase (GlPLK) were distributed. We observed the interaction...

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Autores principales: Park, Eun-Ah, Kim, Juri, Shin, Mee Young, Park, Soon-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256289/
https://www.ncbi.nlm.nih.gov/pubmed/35772734
http://dx.doi.org/10.3347/kjp.2022.60.3.163
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author Park, Eun-Ah
Kim, Juri
Shin, Mee Young
Park, Soon-Jung
author_facet Park, Eun-Ah
Kim, Juri
Shin, Mee Young
Park, Soon-Jung
author_sort Park, Eun-Ah
collection PubMed
description Kinesin-13 (Kin-13), a depolymerizer of microtubule (MT), has been known to affect the length of Giardia. Giardia Kin-13 (GlKin-13) was localized to axoneme, flagellar tips, and centrosomes, where phosphorylated forms of Giardia polo-like kinase (GlPLK) were distributed. We observed the interaction between GlKin-13 and GlPLK via co-immunoprecipitation using transgenic Giardia cells expressing Myc-tagged GlKin-13, hemagglutinin-tagged GlPLK, and in vitro-synthesized GlKin-13 and GlPLK proteins. In vitro-synthesized GlPLK was demonstrated to auto-phosphorylate and phosphorylate GlKin-13 upon incubation with [γ-(32)P]ATP. Morpholino-mediated depletion of both GlKin-13 and GlPLK caused an extension of flagella and a decreased volume of median bodies in Giardia trophozoites. Our results suggest that GlPLK plays a pertinent role in formation of flagella and median bodies by modulating MT depolymerizing activity of GlKin-13.
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spelling pubmed-92562892022-07-11 Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia Park, Eun-Ah Kim, Juri Shin, Mee Young Park, Soon-Jung Korean J Parasitol Original Article Kinesin-13 (Kin-13), a depolymerizer of microtubule (MT), has been known to affect the length of Giardia. Giardia Kin-13 (GlKin-13) was localized to axoneme, flagellar tips, and centrosomes, where phosphorylated forms of Giardia polo-like kinase (GlPLK) were distributed. We observed the interaction between GlKin-13 and GlPLK via co-immunoprecipitation using transgenic Giardia cells expressing Myc-tagged GlKin-13, hemagglutinin-tagged GlPLK, and in vitro-synthesized GlKin-13 and GlPLK proteins. In vitro-synthesized GlPLK was demonstrated to auto-phosphorylate and phosphorylate GlKin-13 upon incubation with [γ-(32)P]ATP. Morpholino-mediated depletion of both GlKin-13 and GlPLK caused an extension of flagella and a decreased volume of median bodies in Giardia trophozoites. Our results suggest that GlPLK plays a pertinent role in formation of flagella and median bodies by modulating MT depolymerizing activity of GlKin-13. The Korean Society for Parasitology and Tropical Medicine 2022-06 2022-06-30 /pmc/articles/PMC9256289/ /pubmed/35772734 http://dx.doi.org/10.3347/kjp.2022.60.3.163 Text en © 2022, Korean Society for Parasitology and Tropical Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Eun-Ah
Kim, Juri
Shin, Mee Young
Park, Soon-Jung
Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
title Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
title_full Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
title_fullStr Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
title_full_unstemmed Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
title_short Kinesin-13, a Motor Protein, is Regulated by Polo-like Kinase in Giardia lamblia
title_sort kinesin-13, a motor protein, is regulated by polo-like kinase in giardia lamblia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256289/
https://www.ncbi.nlm.nih.gov/pubmed/35772734
http://dx.doi.org/10.3347/kjp.2022.60.3.163
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