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Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants against Candida albicans and Aspergillus niger in West Shewa Zone, Ethiopia

Antimicrobial resistance to commercially available medications has become a global issue, yet there is still the possibility of developing new drugs from medicinal plants. As a result, the aims of the present study were to screen secondary metabolites and to evaluate in vitro antifungal activities o...

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Autores principales: Gizaw, Askale, Marami, Lencho Megersa, Teshome, Ibsa, Sarba, Edilu Jorga, Admasu, Petros, Babele, Dagmawit Atalel, Dilba, Getachew Mulatu, Bune, Wakuma Mitiku, Bayu, Morka Dandecha, Tadesse, Miressa, Abdisa, Kebede
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256430/
https://www.ncbi.nlm.nih.gov/pubmed/35800399
http://dx.doi.org/10.1155/2022/3299146
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author Gizaw, Askale
Marami, Lencho Megersa
Teshome, Ibsa
Sarba, Edilu Jorga
Admasu, Petros
Babele, Dagmawit Atalel
Dilba, Getachew Mulatu
Bune, Wakuma Mitiku
Bayu, Morka Dandecha
Tadesse, Miressa
Abdisa, Kebede
author_facet Gizaw, Askale
Marami, Lencho Megersa
Teshome, Ibsa
Sarba, Edilu Jorga
Admasu, Petros
Babele, Dagmawit Atalel
Dilba, Getachew Mulatu
Bune, Wakuma Mitiku
Bayu, Morka Dandecha
Tadesse, Miressa
Abdisa, Kebede
author_sort Gizaw, Askale
collection PubMed
description Antimicrobial resistance to commercially available medications has become a global issue, yet there is still the possibility of developing new drugs from medicinal plants. As a result, the aims of the present study were to screen secondary metabolites and to evaluate in vitro antifungal activities of Brucea antidysenterica, Aloe vera, and Justicia schimperiana. After the plants were identified, their leaves were collected, washed, dried under the shade, pulverized, and extracted with methanol (99.8%) using the maceration technique. The presence of secondary metabolites in plant extracts was screened using various laboratory protocols. The antifungal activities of the plant extract against reference fungal strains of Candida albicans and Aspergillus niger at concentrations of 200, 100, and 50 mg/mL were assessed using the agar-well diffusion method. Ketoconazole (15 μg) was used as a positive control, while 5% dimethyl sulfoxide and/or 5% Tween 80 were used as negative controls. All tests were conducted in triplicate. Alkaloids, flavonoids, and phenols were secondary metabolites found in all plant extracts. The extract of leaves of B. antidysenterica and J. schimperiana formed a mean zone of inhibition of 15.5 ± 0.5 mm and 15.3 ± 0.58 mm, respectively, against Candida albicans at a concentration of 200 mg/mL, whereas extracts of A. vera leaves formed a 12.3 ± 0.58 mm inhibition zone only against Aspergillus niger at 200 mg/mL. In conclusion, the current study found that B. antidysenterica, A. vera, and J. schimperiana had antifungal activity. In addition, all these plants had a variety of secondary metabolites that possibly have antifungal activities. Studies on in vivo investigations and isolation of specific antifungal compounds from these medicinal plants are suggested.
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spelling pubmed-92564302022-07-06 Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants against Candida albicans and Aspergillus niger in West Shewa Zone, Ethiopia Gizaw, Askale Marami, Lencho Megersa Teshome, Ibsa Sarba, Edilu Jorga Admasu, Petros Babele, Dagmawit Atalel Dilba, Getachew Mulatu Bune, Wakuma Mitiku Bayu, Morka Dandecha Tadesse, Miressa Abdisa, Kebede Adv Pharmacol Pharm Sci Research Article Antimicrobial resistance to commercially available medications has become a global issue, yet there is still the possibility of developing new drugs from medicinal plants. As a result, the aims of the present study were to screen secondary metabolites and to evaluate in vitro antifungal activities of Brucea antidysenterica, Aloe vera, and Justicia schimperiana. After the plants were identified, their leaves were collected, washed, dried under the shade, pulverized, and extracted with methanol (99.8%) using the maceration technique. The presence of secondary metabolites in plant extracts was screened using various laboratory protocols. The antifungal activities of the plant extract against reference fungal strains of Candida albicans and Aspergillus niger at concentrations of 200, 100, and 50 mg/mL were assessed using the agar-well diffusion method. Ketoconazole (15 μg) was used as a positive control, while 5% dimethyl sulfoxide and/or 5% Tween 80 were used as negative controls. All tests were conducted in triplicate. Alkaloids, flavonoids, and phenols were secondary metabolites found in all plant extracts. The extract of leaves of B. antidysenterica and J. schimperiana formed a mean zone of inhibition of 15.5 ± 0.5 mm and 15.3 ± 0.58 mm, respectively, against Candida albicans at a concentration of 200 mg/mL, whereas extracts of A. vera leaves formed a 12.3 ± 0.58 mm inhibition zone only against Aspergillus niger at 200 mg/mL. In conclusion, the current study found that B. antidysenterica, A. vera, and J. schimperiana had antifungal activity. In addition, all these plants had a variety of secondary metabolites that possibly have antifungal activities. Studies on in vivo investigations and isolation of specific antifungal compounds from these medicinal plants are suggested. Hindawi 2022-06-28 /pmc/articles/PMC9256430/ /pubmed/35800399 http://dx.doi.org/10.1155/2022/3299146 Text en Copyright © 2022 Askale Gizaw et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gizaw, Askale
Marami, Lencho Megersa
Teshome, Ibsa
Sarba, Edilu Jorga
Admasu, Petros
Babele, Dagmawit Atalel
Dilba, Getachew Mulatu
Bune, Wakuma Mitiku
Bayu, Morka Dandecha
Tadesse, Miressa
Abdisa, Kebede
Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants against Candida albicans and Aspergillus niger in West Shewa Zone, Ethiopia
title Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants against Candida albicans and Aspergillus niger in West Shewa Zone, Ethiopia
title_full Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants against Candida albicans and Aspergillus niger in West Shewa Zone, Ethiopia
title_fullStr Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants against Candida albicans and Aspergillus niger in West Shewa Zone, Ethiopia
title_full_unstemmed Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants against Candida albicans and Aspergillus niger in West Shewa Zone, Ethiopia
title_short Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants against Candida albicans and Aspergillus niger in West Shewa Zone, Ethiopia
title_sort phytochemical screening and in vitro antifungal activity of selected medicinal plants against candida albicans and aspergillus niger in west shewa zone, ethiopia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256430/
https://www.ncbi.nlm.nih.gov/pubmed/35800399
http://dx.doi.org/10.1155/2022/3299146
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