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In Vitro Mitral Valve Model with Unrestricted Ventricular Access: Using Vacuum to Close the Valve and Enable Static Trans-Mitral Pressure
Current in vitro models of the left heart establish the pressure difference required to close the mitral valve by sealing and pressurizing the ventricular side of the valve, limiting important access to the subvalvular apparatus. This paper describes and evaluates a system that establishes physiolog...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256857/ https://www.ncbi.nlm.nih.gov/pubmed/34993757 http://dx.doi.org/10.1007/s12265-021-10199-5 |
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author | Stephens, Sam E. Kammien, Alexander J. Paris, Jacob C. Applequist, Alexis P. Ingels, Neil B. Jensen, Hanna K. Rodgers, Drew E. Cole, Charles R. Wenk, Jonathan F. Jensen, Morten O. |
author_facet | Stephens, Sam E. Kammien, Alexander J. Paris, Jacob C. Applequist, Alexis P. Ingels, Neil B. Jensen, Hanna K. Rodgers, Drew E. Cole, Charles R. Wenk, Jonathan F. Jensen, Morten O. |
author_sort | Stephens, Sam E. |
collection | PubMed |
description | Current in vitro models of the left heart establish the pressure difference required to close the mitral valve by sealing and pressurizing the ventricular side of the valve, limiting important access to the subvalvular apparatus. This paper describes and evaluates a system that establishes physiological pressure differences across the valve using vacuum on the atrial side. The subvalvular apparatus is open to atmospheric pressure and accessible by tools and sensors, establishing a novel technique for experimentation on atrioventricular valves. Porcine mitral valves were excised and closed by vacuum within the atrial chamber. Images were used to document and analyze closure of the leaflets. Papillary muscle force and regurgitant flow rate were measured to be 4.07 N at 120 mmHg and approximately 12.1 ml/s respectively, both of which are within clinically relevant ranges. The relative ease of these measurements demonstrates the usefulness of improved ventricular access at peak pressure/force closure. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-9256857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-92568572022-11-02 In Vitro Mitral Valve Model with Unrestricted Ventricular Access: Using Vacuum to Close the Valve and Enable Static Trans-Mitral Pressure Stephens, Sam E. Kammien, Alexander J. Paris, Jacob C. Applequist, Alexis P. Ingels, Neil B. Jensen, Hanna K. Rodgers, Drew E. Cole, Charles R. Wenk, Jonathan F. Jensen, Morten O. J Cardiovasc Transl Res Original Article Current in vitro models of the left heart establish the pressure difference required to close the mitral valve by sealing and pressurizing the ventricular side of the valve, limiting important access to the subvalvular apparatus. This paper describes and evaluates a system that establishes physiological pressure differences across the valve using vacuum on the atrial side. The subvalvular apparatus is open to atmospheric pressure and accessible by tools and sensors, establishing a novel technique for experimentation on atrioventricular valves. Porcine mitral valves were excised and closed by vacuum within the atrial chamber. Images were used to document and analyze closure of the leaflets. Papillary muscle force and regurgitant flow rate were measured to be 4.07 N at 120 mmHg and approximately 12.1 ml/s respectively, both of which are within clinically relevant ranges. The relative ease of these measurements demonstrates the usefulness of improved ventricular access at peak pressure/force closure. GRAPHICAL ABSTRACT: [Image: see text] Springer US 2022-01-06 2022 /pmc/articles/PMC9256857/ /pubmed/34993757 http://dx.doi.org/10.1007/s12265-021-10199-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Stephens, Sam E. Kammien, Alexander J. Paris, Jacob C. Applequist, Alexis P. Ingels, Neil B. Jensen, Hanna K. Rodgers, Drew E. Cole, Charles R. Wenk, Jonathan F. Jensen, Morten O. In Vitro Mitral Valve Model with Unrestricted Ventricular Access: Using Vacuum to Close the Valve and Enable Static Trans-Mitral Pressure |
title | In Vitro Mitral Valve Model with Unrestricted Ventricular Access: Using Vacuum to Close the Valve and Enable Static Trans-Mitral Pressure |
title_full | In Vitro Mitral Valve Model with Unrestricted Ventricular Access: Using Vacuum to Close the Valve and Enable Static Trans-Mitral Pressure |
title_fullStr | In Vitro Mitral Valve Model with Unrestricted Ventricular Access: Using Vacuum to Close the Valve and Enable Static Trans-Mitral Pressure |
title_full_unstemmed | In Vitro Mitral Valve Model with Unrestricted Ventricular Access: Using Vacuum to Close the Valve and Enable Static Trans-Mitral Pressure |
title_short | In Vitro Mitral Valve Model with Unrestricted Ventricular Access: Using Vacuum to Close the Valve and Enable Static Trans-Mitral Pressure |
title_sort | in vitro mitral valve model with unrestricted ventricular access: using vacuum to close the valve and enable static trans-mitral pressure |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256857/ https://www.ncbi.nlm.nih.gov/pubmed/34993757 http://dx.doi.org/10.1007/s12265-021-10199-5 |
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