A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy

This protocol describes how to analyze C.albicans biofilm using transmission electron microscopy. We present two approaches to observe the ultrastructure of fungal cells within unperturbed biofilms, as well as an immunogold labeling procedure. This approach maintains the architecture of the fungal b...

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Detalles Bibliográficos
Autores principales: Mourer, Thierry, Sachse, Martin, Gazi, Anastasia D., d’Enfert, Christophe, Bachellier-Bassi, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256947/
https://www.ncbi.nlm.nih.gov/pubmed/35779259
http://dx.doi.org/10.1016/j.xpro.2022.101514
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author Mourer, Thierry
Sachse, Martin
Gazi, Anastasia D.
d’Enfert, Christophe
Bachellier-Bassi, Sophie
author_facet Mourer, Thierry
Sachse, Martin
Gazi, Anastasia D.
d’Enfert, Christophe
Bachellier-Bassi, Sophie
author_sort Mourer, Thierry
collection PubMed
description This protocol describes how to analyze C.albicans biofilm using transmission electron microscopy. We present two approaches to observe the ultrastructure of fungal cells within unperturbed biofilms, as well as an immunogold labeling procedure. This approach maintains the architecture of the fungal biofilm close to its native state by growing C. albicans biofilm on a plastic surface. After the freeze substitution procedure, classical transmission electron microscopy or electron tomography will allow the ultrastructural analysis of the microbial community.
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spelling pubmed-92569472022-07-07 A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy Mourer, Thierry Sachse, Martin Gazi, Anastasia D. d’Enfert, Christophe Bachellier-Bassi, Sophie STAR Protoc Protocol This protocol describes how to analyze C.albicans biofilm using transmission electron microscopy. We present two approaches to observe the ultrastructure of fungal cells within unperturbed biofilms, as well as an immunogold labeling procedure. This approach maintains the architecture of the fungal biofilm close to its native state by growing C. albicans biofilm on a plastic surface. After the freeze substitution procedure, classical transmission electron microscopy or electron tomography will allow the ultrastructural analysis of the microbial community. Elsevier 2022-07-01 /pmc/articles/PMC9256947/ /pubmed/35779259 http://dx.doi.org/10.1016/j.xpro.2022.101514 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Mourer, Thierry
Sachse, Martin
Gazi, Anastasia D.
d’Enfert, Christophe
Bachellier-Bassi, Sophie
A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy
title A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy
title_full A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy
title_fullStr A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy
title_full_unstemmed A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy
title_short A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy
title_sort protocol for ultrastructural study of candida albicans biofilm using transmission electron microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256947/
https://www.ncbi.nlm.nih.gov/pubmed/35779259
http://dx.doi.org/10.1016/j.xpro.2022.101514
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