Cargando…

Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold

Major traumatic tissue defects are common clinical problems often complicated by infection and local vascular dysfunction, processes which hinder the healing process. Although local application of growth factors or stem cells through various tissue engineering techniques are promising methods for th...

Descripción completa

Detalles Bibliográficos
Autores principales: Xue, Hang, Zhang, Zhenhe, Lin, Ze, Su, Jin, Panayi, Adriana C., Xiong, Yuan, Hu, Liangcong, Hu, Yiqiang, Chen, Lang, Yan, Chenchen, Xie, Xudong, Shi, Yusheng, Zhou, Wu, Mi, Bobin, Liu, Guohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256949/
https://www.ncbi.nlm.nih.gov/pubmed/35845319
http://dx.doi.org/10.1016/j.bioactmat.2022.05.023
_version_ 1784741226483810304
author Xue, Hang
Zhang, Zhenhe
Lin, Ze
Su, Jin
Panayi, Adriana C.
Xiong, Yuan
Hu, Liangcong
Hu, Yiqiang
Chen, Lang
Yan, Chenchen
Xie, Xudong
Shi, Yusheng
Zhou, Wu
Mi, Bobin
Liu, Guohui
author_facet Xue, Hang
Zhang, Zhenhe
Lin, Ze
Su, Jin
Panayi, Adriana C.
Xiong, Yuan
Hu, Liangcong
Hu, Yiqiang
Chen, Lang
Yan, Chenchen
Xie, Xudong
Shi, Yusheng
Zhou, Wu
Mi, Bobin
Liu, Guohui
author_sort Xue, Hang
collection PubMed
description Major traumatic tissue defects are common clinical problems often complicated by infection and local vascular dysfunction, processes which hinder the healing process. Although local application of growth factors or stem cells through various tissue engineering techniques are promising methods for the repair of tissue defects, limitations in their clinical application exist. Herein, we synthesized multifaceted nanohybrids composed of Quaternized chitosan (QCS), Graphene oxide (GO), and Polydopamine (PDA; QCS-GO-PDA). Covalent grafting of QCS and GO at a mass ratio of 5:1 (5QCS-1GO) displayed excellent biocompatibility and enhanced osteogenic ability, while addition of PDA (5QCS-1GO-PDA) reduced the level of reactive oxygen species (ROS). 5QCS-1GO-PDA was able to achieve wound tissue regeneration by reducing the inflammatory response and enhancing angiogenesis. Furthermore, Polylactic acid/hydroxyapatite (PLA/HA) composite scaffolds were printed using Selective Laser Sintering (SLS) and the hybrid nanomaterial (5QCS-1GO-PDA) was used to coat the PLA/HA scaffold (5QCS-1GO-PDA@PLA/HA) to be used for rapid bone regeneration. 5QCS-1GO-PDA not only improved angiogenesis and osteogenic differentiation, but also induced M2-type polarization of macrophages and promoted bone regeneration via the BMP2/BMPRs/Smads/Runx2 signaling pathway. The bidirectional enhanced healing ability of the multifaceted nanohybrids 5QCS-1GO-PDA provides a promising method of effectively treating tissue defects.
format Online
Article
Text
id pubmed-9256949
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher KeAi Publishing
record_format MEDLINE/PubMed
spelling pubmed-92569492022-07-15 Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold Xue, Hang Zhang, Zhenhe Lin, Ze Su, Jin Panayi, Adriana C. Xiong, Yuan Hu, Liangcong Hu, Yiqiang Chen, Lang Yan, Chenchen Xie, Xudong Shi, Yusheng Zhou, Wu Mi, Bobin Liu, Guohui Bioact Mater Article Major traumatic tissue defects are common clinical problems often complicated by infection and local vascular dysfunction, processes which hinder the healing process. Although local application of growth factors or stem cells through various tissue engineering techniques are promising methods for the repair of tissue defects, limitations in their clinical application exist. Herein, we synthesized multifaceted nanohybrids composed of Quaternized chitosan (QCS), Graphene oxide (GO), and Polydopamine (PDA; QCS-GO-PDA). Covalent grafting of QCS and GO at a mass ratio of 5:1 (5QCS-1GO) displayed excellent biocompatibility and enhanced osteogenic ability, while addition of PDA (5QCS-1GO-PDA) reduced the level of reactive oxygen species (ROS). 5QCS-1GO-PDA was able to achieve wound tissue regeneration by reducing the inflammatory response and enhancing angiogenesis. Furthermore, Polylactic acid/hydroxyapatite (PLA/HA) composite scaffolds were printed using Selective Laser Sintering (SLS) and the hybrid nanomaterial (5QCS-1GO-PDA) was used to coat the PLA/HA scaffold (5QCS-1GO-PDA@PLA/HA) to be used for rapid bone regeneration. 5QCS-1GO-PDA not only improved angiogenesis and osteogenic differentiation, but also induced M2-type polarization of macrophages and promoted bone regeneration via the BMP2/BMPRs/Smads/Runx2 signaling pathway. The bidirectional enhanced healing ability of the multifaceted nanohybrids 5QCS-1GO-PDA provides a promising method of effectively treating tissue defects. KeAi Publishing 2022-06-02 /pmc/articles/PMC9256949/ /pubmed/35845319 http://dx.doi.org/10.1016/j.bioactmat.2022.05.023 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Xue, Hang
Zhang, Zhenhe
Lin, Ze
Su, Jin
Panayi, Adriana C.
Xiong, Yuan
Hu, Liangcong
Hu, Yiqiang
Chen, Lang
Yan, Chenchen
Xie, Xudong
Shi, Yusheng
Zhou, Wu
Mi, Bobin
Liu, Guohui
Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold
title Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold
title_full Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold
title_fullStr Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold
title_full_unstemmed Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold
title_short Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold
title_sort enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256949/
https://www.ncbi.nlm.nih.gov/pubmed/35845319
http://dx.doi.org/10.1016/j.bioactmat.2022.05.023
work_keys_str_mv AT xuehang enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT zhangzhenhe enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT linze enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT sujin enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT panayiadrianac enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT xiongyuan enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT huliangcong enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT huyiqiang enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT chenlang enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT yanchenchen enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT xiexudong enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT shiyusheng enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT zhouwu enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT mibobin enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold
AT liuguohui enhancedtissueregenerationthroughimmunomodulationofangiogenesisandosteogenesiswithamultifacetednanohybridmodifiedbioactivescaffold