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Establishment of an Efficient Genome Editing System in Lettuce Without Sacrificing Specificity

The efficiency of the CRISPR/Cas9 genome editing system remains limited in many crops. Utilizing strong promoters to boost the expression level of Cas9 are commonly used to improve the editing efficiency. However, these strategies also increase the risk of off-target mutation. Here, we developed a n...

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Detalles Bibliográficos
Autores principales: Pan, Wenbo, Liu, Xue, Li, Dayong, Zhang, Huawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257259/
https://www.ncbi.nlm.nih.gov/pubmed/35812897
http://dx.doi.org/10.3389/fpls.2022.930592