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Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India

An oomycetous fungus Phytophthora causing fruit rot is the most devastating disease of arecanut in different agro-climatic zones of Karnataka with varied climatic profiles. The main aim of this investigation was to characterize the geo-distant Phytophthora populations infecting arecanut using robust...

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Autores principales: Patil, Balanagouda, Hegde, Vinayaka, Sridhara, Shankarappa, Pandian, R. Thava Prakasa, Thube, Shivaji H., Karyath Palliath, Gangaraj, Gangurde, Sunil S., Jha, Prakash Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257421/
https://www.ncbi.nlm.nih.gov/pubmed/35813115
http://dx.doi.org/10.1016/j.sjbs.2022.103341
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author Patil, Balanagouda
Hegde, Vinayaka
Sridhara, Shankarappa
Pandian, R. Thava Prakasa
Thube, Shivaji H.
Karyath Palliath, Gangaraj
Gangurde, Sunil S.
Jha, Prakash Kumar
author_facet Patil, Balanagouda
Hegde, Vinayaka
Sridhara, Shankarappa
Pandian, R. Thava Prakasa
Thube, Shivaji H.
Karyath Palliath, Gangaraj
Gangurde, Sunil S.
Jha, Prakash Kumar
author_sort Patil, Balanagouda
collection PubMed
description An oomycetous fungus Phytophthora causing fruit rot is the most devastating disease of arecanut in different agro-climatic zones of Karnataka with varied climatic profiles. The main aim of this investigation was to characterize the geo-distant Phytophthora populations infecting arecanut using robust morphological, multi-gene phylogeny and haplotype analysis. A total of 48 geo-distant fruit rot infected samples were collected during the South-West monsoon of 2017–19. Pure culture of the suspected pathogen was isolated from the infected nuts and pathogenic ability was confirmed and characterized. Colony morphology revealed typical whitish mycelium with stellate or petalloid pattern and appearance with torulose hyphae. Sporangia were caducous, semipapillate or papillate, globose, ellipsoid or ovoid-obpyriform in shape and sporangiophores were irregularly branched or simple sympodial in nature. Subsequent multi-gene phylogeny (ITS, β-tub, TEF-1α and Cox-II) and sequence analysis confirmed the identity of oomycete as Phytophthora meadii which is predominant across the regions studied. We identified 49 haplotypes representing the higher haplotype diversity with varying relative haplotype frequency. Comprehensive study confirmed the existence of substantial variability among geo-distant populations (n = 48) of P. meadii. The knowledge on population dynamics of the pathogen causing fruit rot of arecanut generated from this investigation would aid in developing appropriate disease management strategies to curtail its further occurrence and spread in arecanut ecosystem.
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spelling pubmed-92574212022-07-07 Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India Patil, Balanagouda Hegde, Vinayaka Sridhara, Shankarappa Pandian, R. Thava Prakasa Thube, Shivaji H. Karyath Palliath, Gangaraj Gangurde, Sunil S. Jha, Prakash Kumar Saudi J Biol Sci Original Article An oomycetous fungus Phytophthora causing fruit rot is the most devastating disease of arecanut in different agro-climatic zones of Karnataka with varied climatic profiles. The main aim of this investigation was to characterize the geo-distant Phytophthora populations infecting arecanut using robust morphological, multi-gene phylogeny and haplotype analysis. A total of 48 geo-distant fruit rot infected samples were collected during the South-West monsoon of 2017–19. Pure culture of the suspected pathogen was isolated from the infected nuts and pathogenic ability was confirmed and characterized. Colony morphology revealed typical whitish mycelium with stellate or petalloid pattern and appearance with torulose hyphae. Sporangia were caducous, semipapillate or papillate, globose, ellipsoid or ovoid-obpyriform in shape and sporangiophores were irregularly branched or simple sympodial in nature. Subsequent multi-gene phylogeny (ITS, β-tub, TEF-1α and Cox-II) and sequence analysis confirmed the identity of oomycete as Phytophthora meadii which is predominant across the regions studied. We identified 49 haplotypes representing the higher haplotype diversity with varying relative haplotype frequency. Comprehensive study confirmed the existence of substantial variability among geo-distant populations (n = 48) of P. meadii. The knowledge on population dynamics of the pathogen causing fruit rot of arecanut generated from this investigation would aid in developing appropriate disease management strategies to curtail its further occurrence and spread in arecanut ecosystem. Elsevier 2022-08 2022-06-16 /pmc/articles/PMC9257421/ /pubmed/35813115 http://dx.doi.org/10.1016/j.sjbs.2022.103341 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Patil, Balanagouda
Hegde, Vinayaka
Sridhara, Shankarappa
Pandian, R. Thava Prakasa
Thube, Shivaji H.
Karyath Palliath, Gangaraj
Gangurde, Sunil S.
Jha, Prakash Kumar
Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India
title Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India
title_full Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India
title_fullStr Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India
title_full_unstemmed Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India
title_short Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India
title_sort multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, phytophthora meadii (mcrae) associated with fruit rot disease of arecanut in india
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257421/
https://www.ncbi.nlm.nih.gov/pubmed/35813115
http://dx.doi.org/10.1016/j.sjbs.2022.103341
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