Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India

Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1–3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reve...

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Autores principales: Gaur, Bharti, Saha, Siddhartha, Iuliano, A. Danielle, Rai, Sanjay K., Krishnan, Anand, Jain, Seema, Whitaker, Brett, Winchell, Jonas, Lal, Renu B., Broor, Shobha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257431/
https://www.ncbi.nlm.nih.gov/pubmed/31424019
http://dx.doi.org/10.4103/ijmm.IJMM_18_146
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author Gaur, Bharti
Saha, Siddhartha
Iuliano, A. Danielle
Rai, Sanjay K.
Krishnan, Anand
Jain, Seema
Whitaker, Brett
Winchell, Jonas
Lal, Renu B.
Broor, Shobha
author_facet Gaur, Bharti
Saha, Siddhartha
Iuliano, A. Danielle
Rai, Sanjay K.
Krishnan, Anand
Jain, Seema
Whitaker, Brett
Winchell, Jonas
Lal, Renu B.
Broor, Shobha
author_sort Gaur, Bharti
collection PubMed
description Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1–3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%–100%) and specificity (98%–100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
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spelling pubmed-92574312022-07-06 Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India Gaur, Bharti Saha, Siddhartha Iuliano, A. Danielle Rai, Sanjay K. Krishnan, Anand Jain, Seema Whitaker, Brett Winchell, Jonas Lal, Renu B. Broor, Shobha Indian J Med Microbiol Article Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1–3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%–100%) and specificity (98%–100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations. 2019 /pmc/articles/PMC9257431/ /pubmed/31424019 http://dx.doi.org/10.4103/ijmm.IJMM_18_146 Text en https://creativecommons.org/licenses/by-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 4.0 License, which allows others remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Article
Gaur, Bharti
Saha, Siddhartha
Iuliano, A. Danielle
Rai, Sanjay K.
Krishnan, Anand
Jain, Seema
Whitaker, Brett
Winchell, Jonas
Lal, Renu B.
Broor, Shobha
Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India
title Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India
title_full Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India
title_fullStr Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India
title_full_unstemmed Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India
title_short Use of TaqMan Array Card for the Detection of Respiratory Viral Pathogens in Children under 5 Years Old Hospitalised with Acute Medical Illness in Ballabgarh, Haryana, India
title_sort use of taqman array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in ballabgarh, haryana, india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257431/
https://www.ncbi.nlm.nih.gov/pubmed/31424019
http://dx.doi.org/10.4103/ijmm.IJMM_18_146
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