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A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing

Due to the complexity of bioactive ingredients in biological samples, the screening of target proteins is a complex process. Herein, a feasible strategy for directing protein immobilization on silica magnetic beads for ligand fishing based on SpyTag/SpyCatcher (ST/SC)-mediated anchoring is presented...

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Detalles Bibliográficos
Autores principales:	Yi, Yu, Hu, Jianming, Ding, Shenwei, Mei, Jianfeng, Wang, Xudong, Zhang, Yanlu, Chen, Jianshu, Ying, Guoqing
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Xi'an Jiaotong University 2022
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257657/ https://www.ncbi.nlm.nih.gov/pubmed/35811616 http://dx.doi.org/10.1016/j.jpha.2021.07.008

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