Cargando…
Low potassium disrupt intestinal barrier and result in bacterial translocation
BACKGROUND: Bacterial translocation was observed in critical illness and patients with chronic diseases such as liver cirrhosis and chronic kidney disease (CKD). Hypokalemia is a common complication in these diseases. Whether low potassium diet may increase intestinal permeability and result in bact...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258207/ https://www.ncbi.nlm.nih.gov/pubmed/35794599 http://dx.doi.org/10.1186/s12967-022-03499-0 |
_version_ | 1784741494574284800 |
---|---|
author | Wu, Haishan Huang, Rong Fan, Jinjin Luo, Ning Yang, Xiao |
author_facet | Wu, Haishan Huang, Rong Fan, Jinjin Luo, Ning Yang, Xiao |
author_sort | Wu, Haishan |
collection | PubMed |
description | BACKGROUND: Bacterial translocation was observed in critical illness and patients with chronic diseases such as liver cirrhosis and chronic kidney disease (CKD). Hypokalemia is a common complication in these diseases. Whether low potassium diet may increase intestinal permeability and result in bacterial translocation lack of evidence. The present study was aimed to investigate the potential effects of LK on intestinal permeability. METHODS: Grade 8-week-old male Bal B/C mice were randomly placed either on a normal potassium (NK) mouse chow or a low potassium (LK) diet for 28 days. Intestinal permeability and expression of tight junction proteins were compared between the two groups. RESULTS: Compared with the NK group, the mice in LK group had significantly lower serum potassium level, increased levels of plasmas endotoxin and plasma d-lactate. The bacterial translocation was higher and in occurred mainly in mesenteric lymph nodes (MLN), liver and spleen. The pathologic change of small intestine was obvious with thinner villus lamina propria, shorter crypt depth and thinner intestinal wall. Slight increases in the expression of proteins and mRNA levels of both claudin-1 and claudin-2 were observed in LK group. CONCLUSIONS: Low potassium diet could increase intestinal permeability and thereby lead to bacterial translocation, which was suspected to result from impaired intestinal epithelial barrier and biological barrier. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-022-03499-0. |
format | Online Article Text |
id | pubmed-9258207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92582072022-07-07 Low potassium disrupt intestinal barrier and result in bacterial translocation Wu, Haishan Huang, Rong Fan, Jinjin Luo, Ning Yang, Xiao J Transl Med Research BACKGROUND: Bacterial translocation was observed in critical illness and patients with chronic diseases such as liver cirrhosis and chronic kidney disease (CKD). Hypokalemia is a common complication in these diseases. Whether low potassium diet may increase intestinal permeability and result in bacterial translocation lack of evidence. The present study was aimed to investigate the potential effects of LK on intestinal permeability. METHODS: Grade 8-week-old male Bal B/C mice were randomly placed either on a normal potassium (NK) mouse chow or a low potassium (LK) diet for 28 days. Intestinal permeability and expression of tight junction proteins were compared between the two groups. RESULTS: Compared with the NK group, the mice in LK group had significantly lower serum potassium level, increased levels of plasmas endotoxin and plasma d-lactate. The bacterial translocation was higher and in occurred mainly in mesenteric lymph nodes (MLN), liver and spleen. The pathologic change of small intestine was obvious with thinner villus lamina propria, shorter crypt depth and thinner intestinal wall. Slight increases in the expression of proteins and mRNA levels of both claudin-1 and claudin-2 were observed in LK group. CONCLUSIONS: Low potassium diet could increase intestinal permeability and thereby lead to bacterial translocation, which was suspected to result from impaired intestinal epithelial barrier and biological barrier. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-022-03499-0. BioMed Central 2022-07-06 /pmc/articles/PMC9258207/ /pubmed/35794599 http://dx.doi.org/10.1186/s12967-022-03499-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wu, Haishan Huang, Rong Fan, Jinjin Luo, Ning Yang, Xiao Low potassium disrupt intestinal barrier and result in bacterial translocation |
title | Low potassium disrupt intestinal barrier and result in bacterial translocation |
title_full | Low potassium disrupt intestinal barrier and result in bacterial translocation |
title_fullStr | Low potassium disrupt intestinal barrier and result in bacterial translocation |
title_full_unstemmed | Low potassium disrupt intestinal barrier and result in bacterial translocation |
title_short | Low potassium disrupt intestinal barrier and result in bacterial translocation |
title_sort | low potassium disrupt intestinal barrier and result in bacterial translocation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258207/ https://www.ncbi.nlm.nih.gov/pubmed/35794599 http://dx.doi.org/10.1186/s12967-022-03499-0 |
work_keys_str_mv | AT wuhaishan lowpotassiumdisruptintestinalbarrierandresultinbacterialtranslocation AT huangrong lowpotassiumdisruptintestinalbarrierandresultinbacterialtranslocation AT fanjinjin lowpotassiumdisruptintestinalbarrierandresultinbacterialtranslocation AT luoning lowpotassiumdisruptintestinalbarrierandresultinbacterialtranslocation AT yangxiao lowpotassiumdisruptintestinalbarrierandresultinbacterialtranslocation |