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Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method
OBJECTIVE: Acute myeloid leukemia is caused by the clonal proliferation of undifferentiated myeloid hematopoietic precursors. AML prognosis is highly involved in the treatment response and is determined by mutations in several genes such as N-RAS. This study aims to identify the distribution of comm...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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West Asia Organization for Cancer Prevention
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258652/ https://www.ncbi.nlm.nih.gov/pubmed/35092380 http://dx.doi.org/10.31557/APJCP.2022.23.1.125 |
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author | Zaka Khosravi, Saeed Moonesi, Mohammadreza Moradabadi, Alireza Rajaeinejad, Mohsen Heidari, Mohammad Foad Noroozi-Aghideh, Ali |
author_facet | Zaka Khosravi, Saeed Moonesi, Mohammadreza Moradabadi, Alireza Rajaeinejad, Mohsen Heidari, Mohammad Foad Noroozi-Aghideh, Ali |
author_sort | Zaka Khosravi, Saeed |
collection | PubMed |
description | OBJECTIVE: Acute myeloid leukemia is caused by the clonal proliferation of undifferentiated myeloid hematopoietic precursors. AML prognosis is highly involved in the treatment response and is determined by mutations in several genes such as N-RAS. This study aims to identify the distribution of common N-RAS mutations (codons 12, 13, and 61) in AML patients using the HRM method and confirm this method’s efficiency for mutation detection by comparing its results with the sequencing data as the Gold standard method. METHODS: Peripheral blood samples were taken from 50 newly diagnosed AML patients. Mononuclear cells were isolated from samples, and DNA was extracted. Then, mutation detection was investigated using the HRM method. Efficacy of the HRM method in mutation detection was determined in comparison with direct sequencing. RESULTS: N-RAS mutations were detected in 7 of the 50 samples (14%). Most of the mutations were found in codon 12 (57.14%), and 28.57% and 14.28% of mutations were in codons 61 and 13, respectively. There was no statistically significant association between patients’ demographic data and HRM results. CONCLUSION: According to mutation detection results and the HRM results confirmation with the sequencing method, this method can be introduced as an efficient, low-cost, and fast method for detecting common mutations. |
format | Online Article Text |
id | pubmed-9258652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-92586522022-07-06 Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method Zaka Khosravi, Saeed Moonesi, Mohammadreza Moradabadi, Alireza Rajaeinejad, Mohsen Heidari, Mohammad Foad Noroozi-Aghideh, Ali Asian Pac J Cancer Prev Research Article OBJECTIVE: Acute myeloid leukemia is caused by the clonal proliferation of undifferentiated myeloid hematopoietic precursors. AML prognosis is highly involved in the treatment response and is determined by mutations in several genes such as N-RAS. This study aims to identify the distribution of common N-RAS mutations (codons 12, 13, and 61) in AML patients using the HRM method and confirm this method’s efficiency for mutation detection by comparing its results with the sequencing data as the Gold standard method. METHODS: Peripheral blood samples were taken from 50 newly diagnosed AML patients. Mononuclear cells were isolated from samples, and DNA was extracted. Then, mutation detection was investigated using the HRM method. Efficacy of the HRM method in mutation detection was determined in comparison with direct sequencing. RESULTS: N-RAS mutations were detected in 7 of the 50 samples (14%). Most of the mutations were found in codon 12 (57.14%), and 28.57% and 14.28% of mutations were in codons 61 and 13, respectively. There was no statistically significant association between patients’ demographic data and HRM results. CONCLUSION: According to mutation detection results and the HRM results confirmation with the sequencing method, this method can be introduced as an efficient, low-cost, and fast method for detecting common mutations. West Asia Organization for Cancer Prevention 2022-01 /pmc/articles/PMC9258652/ /pubmed/35092380 http://dx.doi.org/10.31557/APJCP.2022.23.1.125 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. https://creativecommons.org/licenses/by-nc/4.0/ |
spellingShingle | Research Article Zaka Khosravi, Saeed Moonesi, Mohammadreza Moradabadi, Alireza Rajaeinejad, Mohsen Heidari, Mohammad Foad Noroozi-Aghideh, Ali Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method |
title | Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method |
title_full | Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method |
title_fullStr | Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method |
title_full_unstemmed | Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method |
title_short | Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method |
title_sort | rapid detection of n-ras gene common mutations in acute myeloid leukemia (aml) using high resolution melting (hrm) method |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258652/ https://www.ncbi.nlm.nih.gov/pubmed/35092380 http://dx.doi.org/10.31557/APJCP.2022.23.1.125 |
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