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Role of plasma phosphorylated neurofilament heavy chain (pNfH) in amyotrophic lateral sclerosis
The phosphorylated neurofilament heavy chain (pNfH) is a promising biomarker in amyotrophic lateral sclerosis (ALS). We examined plasma pNfH concentrations in order to corroborate its role as a diagnostic and prognostic biomarker in ALS. Incident ALS cases enrolled in a population‐based registry wer...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258711/ https://www.ncbi.nlm.nih.gov/pubmed/35715961 http://dx.doi.org/10.1111/jcmm.17232 |
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author | Zecca, Chiara Dell’Abate, Maria Teresa Pasculli, Giuseppe Capozzo, Rosa Barone, Roberta Arima, Serena Pollice, Alessio Brescia, Vincenzo Tortelli, Rosanna Logroscino, Giancarlo |
author_facet | Zecca, Chiara Dell’Abate, Maria Teresa Pasculli, Giuseppe Capozzo, Rosa Barone, Roberta Arima, Serena Pollice, Alessio Brescia, Vincenzo Tortelli, Rosanna Logroscino, Giancarlo |
author_sort | Zecca, Chiara |
collection | PubMed |
description | The phosphorylated neurofilament heavy chain (pNfH) is a promising biomarker in amyotrophic lateral sclerosis (ALS). We examined plasma pNfH concentrations in order to corroborate its role as a diagnostic and prognostic biomarker in ALS. Incident ALS cases enrolled in a population‐based registry were retrospectively selected and matched by sex and age with a cohort of healthy volunteers. Plasma pNfH levels were measured by an ELISA kit and correlated with clinical parameters. Discrimination ability of pNfH was tested using receiving operating characteristic (ROC) curves. Kaplan–Meier (KM) analysis and Cox proportional hazard models were used for survival analysis. Plasma pNfH was significantly higher in patients compared to controls. An optimal cut‐off of 39.74 pg/ml discriminated cases from controls with an elevated sensitivity and specificity. Bulbar‐onset cases had higher plasma pNfH compared to spinal onset (p = 0.0033). Furthermore, plasma pNfH positively correlated with disease progression rate (r = 0.19, p = 0.031). Baseline plasma pNfH did not influence survival in our cohort. Our findings confirmed the potential utility of plasma pNfH as a diagnostic biomarker in ALS. However, further studies with longitudinal data are needed to corroborate its prognostic value. |
format | Online Article Text |
id | pubmed-9258711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92587112022-07-11 Role of plasma phosphorylated neurofilament heavy chain (pNfH) in amyotrophic lateral sclerosis Zecca, Chiara Dell’Abate, Maria Teresa Pasculli, Giuseppe Capozzo, Rosa Barone, Roberta Arima, Serena Pollice, Alessio Brescia, Vincenzo Tortelli, Rosanna Logroscino, Giancarlo J Cell Mol Med Original Articles The phosphorylated neurofilament heavy chain (pNfH) is a promising biomarker in amyotrophic lateral sclerosis (ALS). We examined plasma pNfH concentrations in order to corroborate its role as a diagnostic and prognostic biomarker in ALS. Incident ALS cases enrolled in a population‐based registry were retrospectively selected and matched by sex and age with a cohort of healthy volunteers. Plasma pNfH levels were measured by an ELISA kit and correlated with clinical parameters. Discrimination ability of pNfH was tested using receiving operating characteristic (ROC) curves. Kaplan–Meier (KM) analysis and Cox proportional hazard models were used for survival analysis. Plasma pNfH was significantly higher in patients compared to controls. An optimal cut‐off of 39.74 pg/ml discriminated cases from controls with an elevated sensitivity and specificity. Bulbar‐onset cases had higher plasma pNfH compared to spinal onset (p = 0.0033). Furthermore, plasma pNfH positively correlated with disease progression rate (r = 0.19, p = 0.031). Baseline plasma pNfH did not influence survival in our cohort. Our findings confirmed the potential utility of plasma pNfH as a diagnostic biomarker in ALS. However, further studies with longitudinal data are needed to corroborate its prognostic value. John Wiley and Sons Inc. 2022-06-17 2022-07 /pmc/articles/PMC9258711/ /pubmed/35715961 http://dx.doi.org/10.1111/jcmm.17232 Text en © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Zecca, Chiara Dell’Abate, Maria Teresa Pasculli, Giuseppe Capozzo, Rosa Barone, Roberta Arima, Serena Pollice, Alessio Brescia, Vincenzo Tortelli, Rosanna Logroscino, Giancarlo Role of plasma phosphorylated neurofilament heavy chain (pNfH) in amyotrophic lateral sclerosis |
title | Role of plasma phosphorylated neurofilament heavy chain (pNfH) in amyotrophic lateral sclerosis |
title_full | Role of plasma phosphorylated neurofilament heavy chain (pNfH) in amyotrophic lateral sclerosis |
title_fullStr | Role of plasma phosphorylated neurofilament heavy chain (pNfH) in amyotrophic lateral sclerosis |
title_full_unstemmed | Role of plasma phosphorylated neurofilament heavy chain (pNfH) in amyotrophic lateral sclerosis |
title_short | Role of plasma phosphorylated neurofilament heavy chain (pNfH) in amyotrophic lateral sclerosis |
title_sort | role of plasma phosphorylated neurofilament heavy chain (pnfh) in amyotrophic lateral sclerosis |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258711/ https://www.ncbi.nlm.nih.gov/pubmed/35715961 http://dx.doi.org/10.1111/jcmm.17232 |
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