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The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males

Aedes albopictus is one of the most invasive insect species in the world and an effective vector for many important arboviruses. We reported previously that Ae. albopictus Nix (AalNix) is the male-determining factor of this species. However, whether AalNix alone is sufficient to initiate male develo...

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Detalles Bibliográficos
Autores principales: Zhao, Yijie, Jin, Binbin, Liu, Peiwen, Xiao, Xiaolin, Cai, Lijun, Xie, Zhensheng, Kong, Ling, Liu, Tong, Yang, Wenqiang, Wu, Yang, Gu, Jinbao, Tu, Zhijian, James, Anthony A., Chen, Xiao-Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258803/
https://www.ncbi.nlm.nih.gov/pubmed/35737710
http://dx.doi.org/10.1371/journal.pgen.1010280
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author Zhao, Yijie
Jin, Binbin
Liu, Peiwen
Xiao, Xiaolin
Cai, Lijun
Xie, Zhensheng
Kong, Ling
Liu, Tong
Yang, Wenqiang
Wu, Yang
Gu, Jinbao
Tu, Zhijian
James, Anthony A.
Chen, Xiao-Guang
author_facet Zhao, Yijie
Jin, Binbin
Liu, Peiwen
Xiao, Xiaolin
Cai, Lijun
Xie, Zhensheng
Kong, Ling
Liu, Tong
Yang, Wenqiang
Wu, Yang
Gu, Jinbao
Tu, Zhijian
James, Anthony A.
Chen, Xiao-Guang
author_sort Zhao, Yijie
collection PubMed
description Aedes albopictus is one of the most invasive insect species in the world and an effective vector for many important arboviruses. We reported previously that Ae. albopictus Nix (AalNix) is the male-determining factor of this species. However, whether AalNix alone is sufficient to initiate male development is unknown. Transgenic lines that express each of the three AalNix isoforms from the native promoter were obtained using piggyBac transformation. We verified the stable expression of AalNix isoforms in the transgenic lines and confirm that one isoform, AalNix3&4, is sufficient to convert females into fertile males (pseudo-males) that are indistinguishable from wild-type males. We also established a stable sex-converted female mosquito strain, AalNix3&4-♂4-pseudo-male. The pseudo-male mosquitoes can fly and mate normally with wild-type female, although their mating competitiveness is lower than wild-type. This work further clarifies the role of AalNix in the sex determination pathway and will facilitate the development of Ae. albopictus control strategies that rely on male-only releases such as SIT and sex-ratio distortion.
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spelling pubmed-92588032022-07-07 The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males Zhao, Yijie Jin, Binbin Liu, Peiwen Xiao, Xiaolin Cai, Lijun Xie, Zhensheng Kong, Ling Liu, Tong Yang, Wenqiang Wu, Yang Gu, Jinbao Tu, Zhijian James, Anthony A. Chen, Xiao-Guang PLoS Genet Research Article Aedes albopictus is one of the most invasive insect species in the world and an effective vector for many important arboviruses. We reported previously that Ae. albopictus Nix (AalNix) is the male-determining factor of this species. However, whether AalNix alone is sufficient to initiate male development is unknown. Transgenic lines that express each of the three AalNix isoforms from the native promoter were obtained using piggyBac transformation. We verified the stable expression of AalNix isoforms in the transgenic lines and confirm that one isoform, AalNix3&4, is sufficient to convert females into fertile males (pseudo-males) that are indistinguishable from wild-type males. We also established a stable sex-converted female mosquito strain, AalNix3&4-♂4-pseudo-male. The pseudo-male mosquitoes can fly and mate normally with wild-type female, although their mating competitiveness is lower than wild-type. This work further clarifies the role of AalNix in the sex determination pathway and will facilitate the development of Ae. albopictus control strategies that rely on male-only releases such as SIT and sex-ratio distortion. Public Library of Science 2022-06-23 /pmc/articles/PMC9258803/ /pubmed/35737710 http://dx.doi.org/10.1371/journal.pgen.1010280 Text en © 2022 Zhao et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhao, Yijie
Jin, Binbin
Liu, Peiwen
Xiao, Xiaolin
Cai, Lijun
Xie, Zhensheng
Kong, Ling
Liu, Tong
Yang, Wenqiang
Wu, Yang
Gu, Jinbao
Tu, Zhijian
James, Anthony A.
Chen, Xiao-Guang
The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males
title The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males
title_full The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males
title_fullStr The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males
title_full_unstemmed The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males
title_short The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males
title_sort aalnix3&4 isoform is required and sufficient to convert aedes albopictus females into males
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258803/
https://www.ncbi.nlm.nih.gov/pubmed/35737710
http://dx.doi.org/10.1371/journal.pgen.1010280
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