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Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
Successful regeneration requires the coordinated execution of multiple cellular responses to injury. In amputated zebrafish fins, mature osteoblasts dedifferentiate, migrate towards the injury, and form proliferative osteogenic blastema cells. We show that osteoblast migration is preceded by cell el...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259016/ https://www.ncbi.nlm.nih.gov/pubmed/35748539 http://dx.doi.org/10.7554/eLife.77614 |
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author | Sehring, Ivonne Mohammadi, Hossein Falah Haffner-Luntzer, Melanie Ignatius, Anita Huber-Lang, Markus Weidinger, Gilbert |
author_facet | Sehring, Ivonne Mohammadi, Hossein Falah Haffner-Luntzer, Melanie Ignatius, Anita Huber-Lang, Markus Weidinger, Gilbert |
author_sort | Sehring, Ivonne |
collection | PubMed |
description | Successful regeneration requires the coordinated execution of multiple cellular responses to injury. In amputated zebrafish fins, mature osteoblasts dedifferentiate, migrate towards the injury, and form proliferative osteogenic blastema cells. We show that osteoblast migration is preceded by cell elongation and alignment along the proximodistal axis, which require actomyosin, but not microtubule (MT) turnover. Surprisingly, osteoblast dedifferentiation and migration can be uncoupled. Using pharmacological and genetic interventions, we found that NF-ĸB and retinoic acid signalling regulate dedifferentiation without affecting migration, while the complement system and actomyosin dynamics affect migration but not dedifferentiation. Furthermore, by removing bone at two locations within a fin ray, we established an injury model containing two injury sites. We found that osteoblasts dedifferentiate at and migrate towards both sites, while accumulation of osteogenic progenitor cells and regenerative bone formation only occur at the distal-facing injury. Together, these data indicate that osteoblast dedifferentiation and migration represent generic injury responses that are differentially regulated and can occur independently of each other and of regenerative growth. We conclude that successful fin bone regeneration appears to involve the coordinated execution of generic and regeneration-specific responses of osteoblasts to injury. |
format | Online Article Text |
id | pubmed-9259016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-92590162022-07-07 Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses Sehring, Ivonne Mohammadi, Hossein Falah Haffner-Luntzer, Melanie Ignatius, Anita Huber-Lang, Markus Weidinger, Gilbert eLife Cell Biology Successful regeneration requires the coordinated execution of multiple cellular responses to injury. In amputated zebrafish fins, mature osteoblasts dedifferentiate, migrate towards the injury, and form proliferative osteogenic blastema cells. We show that osteoblast migration is preceded by cell elongation and alignment along the proximodistal axis, which require actomyosin, but not microtubule (MT) turnover. Surprisingly, osteoblast dedifferentiation and migration can be uncoupled. Using pharmacological and genetic interventions, we found that NF-ĸB and retinoic acid signalling regulate dedifferentiation without affecting migration, while the complement system and actomyosin dynamics affect migration but not dedifferentiation. Furthermore, by removing bone at two locations within a fin ray, we established an injury model containing two injury sites. We found that osteoblasts dedifferentiate at and migrate towards both sites, while accumulation of osteogenic progenitor cells and regenerative bone formation only occur at the distal-facing injury. Together, these data indicate that osteoblast dedifferentiation and migration represent generic injury responses that are differentially regulated and can occur independently of each other and of regenerative growth. We conclude that successful fin bone regeneration appears to involve the coordinated execution of generic and regeneration-specific responses of osteoblasts to injury. eLife Sciences Publications, Ltd 2022-06-24 /pmc/articles/PMC9259016/ /pubmed/35748539 http://dx.doi.org/10.7554/eLife.77614 Text en © 2022, Sehring et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Sehring, Ivonne Mohammadi, Hossein Falah Haffner-Luntzer, Melanie Ignatius, Anita Huber-Lang, Markus Weidinger, Gilbert Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses |
title | Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses |
title_full | Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses |
title_fullStr | Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses |
title_full_unstemmed | Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses |
title_short | Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses |
title_sort | zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259016/ https://www.ncbi.nlm.nih.gov/pubmed/35748539 http://dx.doi.org/10.7554/eLife.77614 |
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