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Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses

Successful regeneration requires the coordinated execution of multiple cellular responses to injury. In amputated zebrafish fins, mature osteoblasts dedifferentiate, migrate towards the injury, and form proliferative osteogenic blastema cells. We show that osteoblast migration is preceded by cell el...

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Autores principales: Sehring, Ivonne, Mohammadi, Hossein Falah, Haffner-Luntzer, Melanie, Ignatius, Anita, Huber-Lang, Markus, Weidinger, Gilbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259016/
https://www.ncbi.nlm.nih.gov/pubmed/35748539
http://dx.doi.org/10.7554/eLife.77614
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author Sehring, Ivonne
Mohammadi, Hossein Falah
Haffner-Luntzer, Melanie
Ignatius, Anita
Huber-Lang, Markus
Weidinger, Gilbert
author_facet Sehring, Ivonne
Mohammadi, Hossein Falah
Haffner-Luntzer, Melanie
Ignatius, Anita
Huber-Lang, Markus
Weidinger, Gilbert
author_sort Sehring, Ivonne
collection PubMed
description Successful regeneration requires the coordinated execution of multiple cellular responses to injury. In amputated zebrafish fins, mature osteoblasts dedifferentiate, migrate towards the injury, and form proliferative osteogenic blastema cells. We show that osteoblast migration is preceded by cell elongation and alignment along the proximodistal axis, which require actomyosin, but not microtubule (MT) turnover. Surprisingly, osteoblast dedifferentiation and migration can be uncoupled. Using pharmacological and genetic interventions, we found that NF-ĸB and retinoic acid signalling regulate dedifferentiation without affecting migration, while the complement system and actomyosin dynamics affect migration but not dedifferentiation. Furthermore, by removing bone at two locations within a fin ray, we established an injury model containing two injury sites. We found that osteoblasts dedifferentiate at and migrate towards both sites, while accumulation of osteogenic progenitor cells and regenerative bone formation only occur at the distal-facing injury. Together, these data indicate that osteoblast dedifferentiation and migration represent generic injury responses that are differentially regulated and can occur independently of each other and of regenerative growth. We conclude that successful fin bone regeneration appears to involve the coordinated execution of generic and regeneration-specific responses of osteoblasts to injury.
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spelling pubmed-92590162022-07-07 Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses Sehring, Ivonne Mohammadi, Hossein Falah Haffner-Luntzer, Melanie Ignatius, Anita Huber-Lang, Markus Weidinger, Gilbert eLife Cell Biology Successful regeneration requires the coordinated execution of multiple cellular responses to injury. In amputated zebrafish fins, mature osteoblasts dedifferentiate, migrate towards the injury, and form proliferative osteogenic blastema cells. We show that osteoblast migration is preceded by cell elongation and alignment along the proximodistal axis, which require actomyosin, but not microtubule (MT) turnover. Surprisingly, osteoblast dedifferentiation and migration can be uncoupled. Using pharmacological and genetic interventions, we found that NF-ĸB and retinoic acid signalling regulate dedifferentiation without affecting migration, while the complement system and actomyosin dynamics affect migration but not dedifferentiation. Furthermore, by removing bone at two locations within a fin ray, we established an injury model containing two injury sites. We found that osteoblasts dedifferentiate at and migrate towards both sites, while accumulation of osteogenic progenitor cells and regenerative bone formation only occur at the distal-facing injury. Together, these data indicate that osteoblast dedifferentiation and migration represent generic injury responses that are differentially regulated and can occur independently of each other and of regenerative growth. We conclude that successful fin bone regeneration appears to involve the coordinated execution of generic and regeneration-specific responses of osteoblasts to injury. eLife Sciences Publications, Ltd 2022-06-24 /pmc/articles/PMC9259016/ /pubmed/35748539 http://dx.doi.org/10.7554/eLife.77614 Text en © 2022, Sehring et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Sehring, Ivonne
Mohammadi, Hossein Falah
Haffner-Luntzer, Melanie
Ignatius, Anita
Huber-Lang, Markus
Weidinger, Gilbert
Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
title Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
title_full Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
title_fullStr Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
title_full_unstemmed Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
title_short Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
title_sort zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259016/
https://www.ncbi.nlm.nih.gov/pubmed/35748539
http://dx.doi.org/10.7554/eLife.77614
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