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Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD
PURPOSE: Pulmonary surfactant proteins A (SP-A) and D (SP-D) are lectins, involved in host defense and regulation of pulmonary inflammatory response. However, studies on the assessment of COPD progress are limited. PATIENTS AND METHODS: Pulmonary surfactant proteins were obtained from the COPD mouse...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259505/ https://www.ncbi.nlm.nih.gov/pubmed/35811742 http://dx.doi.org/10.2147/COPD.S366988 |
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author | Lv, Mei-Yu Qiang, Li-Xia Wang, Bao-Cai Zhang, Yue-Peng Li, Zhi-Heng Li, Xiang-Shun Jin, Ling-Ling Jin, Shou-De |
author_facet | Lv, Mei-Yu Qiang, Li-Xia Wang, Bao-Cai Zhang, Yue-Peng Li, Zhi-Heng Li, Xiang-Shun Jin, Ling-Ling Jin, Shou-De |
author_sort | Lv, Mei-Yu |
collection | PubMed |
description | PURPOSE: Pulmonary surfactant proteins A (SP-A) and D (SP-D) are lectins, involved in host defense and regulation of pulmonary inflammatory response. However, studies on the assessment of COPD progress are limited. PATIENTS AND METHODS: Pulmonary surfactant proteins were obtained from the COPD mouse model induced by cigarette and lipopolysaccharide, and the specimens of peripheral blood and bronchoalveolar lavage (BALF) in COPD populations. H&E staining and RT-PCR were performed to demonstrate the successfully established of the mouse model. The expression of SP-A and SP-D in mice was detected by Western Blot and immunohistochemistry, while the proteins in human samples were measured by ELISA. Pulmonary function test, inflammatory factors (CRP, WBC, NLR, PCT, EOS, PLT), dyspnea index score (mMRC and CAT), length of hospital stay, incidence of complications and ventilator use were collected to assess airway remodeling and progression of COPD. RESULTS: COPD model mice with emphysema and airway wall thickening were more prone to have decreased SP-A, SP-D and increased TNF-α, TGF-β, and NF-kb in lung tissue. In humans, SP-A and SP-D decreased in BALF, but increased in serum. The serum SP-A and SP-D were negatively correlated with FVC, FEV1, FEV1/FVC, and positively correlated with CRP, WBC, NLR, mMRC and CAT scores (P < 0.05, respectively). The lower the SP-A and SP-D in BALF, the worse the lung function and the increased probability of complications and ventilator use. Moreover, the same trend emerged in COPD patients grouped according to GOLD severity grade (Gold 1–2 group vs Gold 3–4 group). The worse the patient’s condition, the more pronounced the change. CONCLUSION: This study suggests that SP-A and SP-D may be related to the progression and prognostic evaluation of COPD in terms of airway remodeling, inflammatory response and clinical symptoms, and emphasizes the necessity of future studies of surfactant protein markers in COPD. |
format | Online Article Text |
id | pubmed-9259505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-92595052022-07-08 Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD Lv, Mei-Yu Qiang, Li-Xia Wang, Bao-Cai Zhang, Yue-Peng Li, Zhi-Heng Li, Xiang-Shun Jin, Ling-Ling Jin, Shou-De Int J Chron Obstruct Pulmon Dis Original Research PURPOSE: Pulmonary surfactant proteins A (SP-A) and D (SP-D) are lectins, involved in host defense and regulation of pulmonary inflammatory response. However, studies on the assessment of COPD progress are limited. PATIENTS AND METHODS: Pulmonary surfactant proteins were obtained from the COPD mouse model induced by cigarette and lipopolysaccharide, and the specimens of peripheral blood and bronchoalveolar lavage (BALF) in COPD populations. H&E staining and RT-PCR were performed to demonstrate the successfully established of the mouse model. The expression of SP-A and SP-D in mice was detected by Western Blot and immunohistochemistry, while the proteins in human samples were measured by ELISA. Pulmonary function test, inflammatory factors (CRP, WBC, NLR, PCT, EOS, PLT), dyspnea index score (mMRC and CAT), length of hospital stay, incidence of complications and ventilator use were collected to assess airway remodeling and progression of COPD. RESULTS: COPD model mice with emphysema and airway wall thickening were more prone to have decreased SP-A, SP-D and increased TNF-α, TGF-β, and NF-kb in lung tissue. In humans, SP-A and SP-D decreased in BALF, but increased in serum. The serum SP-A and SP-D were negatively correlated with FVC, FEV1, FEV1/FVC, and positively correlated with CRP, WBC, NLR, mMRC and CAT scores (P < 0.05, respectively). The lower the SP-A and SP-D in BALF, the worse the lung function and the increased probability of complications and ventilator use. Moreover, the same trend emerged in COPD patients grouped according to GOLD severity grade (Gold 1–2 group vs Gold 3–4 group). The worse the patient’s condition, the more pronounced the change. CONCLUSION: This study suggests that SP-A and SP-D may be related to the progression and prognostic evaluation of COPD in terms of airway remodeling, inflammatory response and clinical symptoms, and emphasizes the necessity of future studies of surfactant protein markers in COPD. Dove 2022-07-02 /pmc/articles/PMC9259505/ /pubmed/35811742 http://dx.doi.org/10.2147/COPD.S366988 Text en © 2022 Lv et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Lv, Mei-Yu Qiang, Li-Xia Wang, Bao-Cai Zhang, Yue-Peng Li, Zhi-Heng Li, Xiang-Shun Jin, Ling-Ling Jin, Shou-De Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD |
title | Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD |
title_full | Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD |
title_fullStr | Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD |
title_full_unstemmed | Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD |
title_short | Complex Evaluation of Surfactant Protein A and D as Biomarkers for the Severity of COPD |
title_sort | complex evaluation of surfactant protein a and d as biomarkers for the severity of copd |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259505/ https://www.ncbi.nlm.nih.gov/pubmed/35811742 http://dx.doi.org/10.2147/COPD.S366988 |
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