Cargando…

In silico screening and heterologous expression of soluble dimethyl sulfide monooxygenases of microbial origin in Escherichia coli

ABSTRACT: Sequence-based screening has been widely applied in the discovery of novel microbial enzymes. However, majority of the sequences in the genomic databases were annotated using computational approaches and lacks experimental characterization. Hence, the success in obtaining the functional bi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Karaiyan, Prasanth, Chang, Catherine Ching Han, Chan, Eng-Seng, Tey, Beng Ti, Ramanan, Ramakrishnan Nagasundara, Ooi, Chien Wei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2022
Materias:	Biotechnologically Relevant Enzymes and Proteins
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259527/ https://www.ncbi.nlm.nih.gov/pubmed/35713659 http://dx.doi.org/10.1007/s00253-022-12008-8

Ejemplares similares

Investigating the coenzyme specificity of phenylacetone monooxygenase from Thermobifida fusca
por: Dudek, Hanna M., et al.
Publicado: (2010)

Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases
por: Frommhagen, Matthias, et al.
Publicado: (2017)

Expanding the set of rhodococcal Baeyer–Villiger monooxygenases by high-throughput cloning, expression and substrate screening
por: Riebel, A., et al.
Publicado: (2012)

Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase–cyclohexanone monooxygenase fusions
por: Aalbers, Friso S., et al.
Publicado: (2017)

Characterization of cytochrome P450 monooxygenase CYP154H1 from the thermophilic soil bacterium Thermobifida fusca
por: Schallmey, Anett, et al.
Publicado: (2010)

Quinone binding site in a type VI sulfide:quinone oxidoreductase
por: Miklovics, Nikolett, et al.
Publicado: (2022)

A novel galactolipase from a green microalga Chlorella kessleri: purification, characterization, molecular cloning, and heterologous expression
por: Hashiro, Shuhei, et al.
Publicado: (2018)

Heterologous expression and characterization of CpI, OcpA, and novel serine-type carboxypeptidase OcpB from Aspergillus oryzae
por: Morita, Hiroto, et al.
Publicado: (2009)

In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
por: Gonçalves, Jênifer Malheiros, et al.
Publicado: (2022)

In silico analyses and design of a chimeric protein containing epitopes of SpaC, PknG, NanH, and SodC proteins for the control of caseous lymphadenitis
por: Silva, Mara Thais de Oliveira, et al.
Publicado: (2021)

A universal strategy for high-yield production of soluble and functional clostridial collagenases in E. coli
por: Ducka, Paulina, et al.
Publicado: (2009)

SUMO fusion system facilitates soluble expression and high production of bioactive human fibroblast growth factor 23 (FGF23)
por: Liu, Xiaoju, et al.
Publicado: (2012)

Secretion of Streptomyces mobaraensis pro-transglutaminase by coryneform bacteria
por: Itaya, Hiroshi, et al.
Publicado: (2008)

Discovery and characterization of a putrescine oxidase from Rhodococcus erythropolis NCIMB 11540
por: van Hellemond, Erik W., et al.
Publicado: (2008)

Characterization of a modular enzyme of exo-1,5-α-l-arabinofuranosidase and arabinan binding module from Streptomyces avermitilis NBRC14893
por: Ichinose, Hitomi, et al.
Publicado: (2008)

Regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli
por: He, Xian-Zhi, et al.
Publicado: (2008)

Biochemical characterization of the carotenoid 1,2-hydratases (CrtC) from Rubrivivax gelatinosus and Thiocapsa roseopersicina
por: Hiseni, Aida, et al.
Publicado: (2011)

Enhancement of toxin- and virus-neutralizing capacity of single-domain antibody fragments by N-glycosylation
por: Harmsen, M. M., et al.
Publicado: (2009)

A robust and extracellular heme-containing peroxidase from Thermobifida fusca as prototype of a bacterial peroxidase superfamily
por: van Bloois, Edwin, et al.
Publicado: (2009)

In vitro synthesis of heparosan using recombinant Pasteurella multocida heparosan synthase PmHS2
por: Chavaroche, Anaïs A. E., et al.
Publicado: (2009)

The evolution of cyclodextrin glucanotransferase product specificity
por: Kelly, Ronan M., et al.
Publicado: (2009)

Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans
por: Raj, Hans, et al.
Publicado: (2011)

Hot or not? Discovery and characterization of a thermostable alditol oxidase from Acidothermus cellulolyticus 11B
por: Winter, Remko T., et al.
Publicado: (2012)

Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
por: Sygmund, Christoph, et al.
Publicado: (2011)

Identification and characterization of DGA2, an acyltransferase of the DGAT1 acyl-CoA:diacylglycerol acyltransferase family in the oleaginous yeast Yarrowia lipolytica. New insights into the storage lipid metabolism of oleaginous yeasts
por: Beopoulos, Athanasios, et al.
Publicado: (2011)

Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A
por: Sarian, Fean D., et al.
Publicado: (2011)

A genetically engineered protein domain binding to bacterial murein, archaeal pseudomurein, and fungal chitin cell wall material
por: Visweswaran, Ganesh Ram R., et al.
Publicado: (2012)

Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis
por: van Bloois, Edwin, et al.
Publicado: (2009)

Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module
por: Tabuchi, Soichiro, et al.
Publicado: (2010)

Purification, characterization, and cloning of a bifunctional molybdoenzyme with hydratase and alcohol dehydrogenase activity
por: Jin, Jianfeng, et al.
Publicado: (2010)

Synthesis of heparosan oligosaccharides by Pasteurella multocida PmHS2 single-action transferases
por: Chavaroche, Anaïs A. E., et al.
Publicado: (2011)

Identification and characterization of new Δ-17 fatty acid desaturases
por: Xue, Zhixiong, et al.
Publicado: (2012)

A highly thermoactive and salt-tolerant α-amylase isolated from a pilot-plant biogas reactor
por: Jabbour, Dina, et al.
Publicado: (2012)

Human acetyl-CoA carboxylase 2 expressed in silkworm Bombyx mori exhibits posttranslational biotinylation and phosphorylation
por: Hwang, In-Wook, et al.
Publicado: (2014)

Novel CAD-like enzymes from Escherichia coli K-12 as additional tools in chemical production
por: Pick, André, et al.
Publicado: (2012)

4,6-α-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily
por: Leemhuis, Hans, et al.
Publicado: (2012)

Expression and delivery of an endolysin to combat Clostridium perfringens
por: Gervasi, Teresa, et al.
Publicado: (2013)

Asymmetric reduction of ketones and β-keto esters by (S)-1-phenylethanol dehydrogenase from denitrifying bacterium Aromatoleum aromaticum
por: Dudzik, A., et al.
Publicado: (2014)

Evaluation of a recombinant insect-derived amylase performance in simultaneous saccharification and fermentation process with industrial yeasts
por: Celińska, Ewelina, et al.
Publicado: (2015)

Targeting surface-layer proteins with single-domain antibodies: a potential therapeutic approach against Clostridium difficile-associated disease
por: Kandalaft, Hiba, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_k8agucvlf9sqom2tj6jadus809