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The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes
The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace the viral spread and the isolation of COVID-19-infected patients. The detection cap...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259548/ https://www.ncbi.nlm.nih.gov/pubmed/35812012 http://dx.doi.org/10.1155/2022/2594564 |
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author | Luraschi, Roberto Barrera-Avalos, Carlos Vallejos-Vidal, Eva Alarcón, Javiera Mella-Torres, Andrea Hernández, Felipe Inostroza-Molina, Ailen Valdés, Daniel Imarai, Mónica Acuña-Castillo, Claudio Reyes-López, Felipe E. Sandino, Ana María |
author_facet | Luraschi, Roberto Barrera-Avalos, Carlos Vallejos-Vidal, Eva Alarcón, Javiera Mella-Torres, Andrea Hernández, Felipe Inostroza-Molina, Ailen Valdés, Daniel Imarai, Mónica Acuña-Castillo, Claudio Reyes-López, Felipe E. Sandino, Ana María |
author_sort | Luraschi, Roberto |
collection | PubMed |
description | The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace the viral spread and the isolation of COVID-19-infected patients. The detection capacity of viral and internal genes is an essential parameter to consider and analyze during the assay. In this study, we analyze the performance of the two commercial RT-qPCR kits used in Chile, TaqMan™ 2019-nCoV Control Kit v1 (Thermo Fisher) and MaxCov19 (TAAG Genetics), for the COVID-19 diagnosis from nasopharyngeal swab samples (NPSs). Our results show a lower sensitivity of the TAAG kit compared to the Thermo Fisher kit, even in the detection of SARS-CoV-2 mutations associated with its variants. This study reinforces the relevance of evaluating the performance of RT-qPCR kits before being used massively since those with lower sensitivity can generate false negatives and produce outbreaks of local infections. |
format | Online Article Text |
id | pubmed-9259548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-92595482022-07-08 The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes Luraschi, Roberto Barrera-Avalos, Carlos Vallejos-Vidal, Eva Alarcón, Javiera Mella-Torres, Andrea Hernández, Felipe Inostroza-Molina, Ailen Valdés, Daniel Imarai, Mónica Acuña-Castillo, Claudio Reyes-López, Felipe E. Sandino, Ana María Can J Infect Dis Med Microbiol Research Article The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace the viral spread and the isolation of COVID-19-infected patients. The detection capacity of viral and internal genes is an essential parameter to consider and analyze during the assay. In this study, we analyze the performance of the two commercial RT-qPCR kits used in Chile, TaqMan™ 2019-nCoV Control Kit v1 (Thermo Fisher) and MaxCov19 (TAAG Genetics), for the COVID-19 diagnosis from nasopharyngeal swab samples (NPSs). Our results show a lower sensitivity of the TAAG kit compared to the Thermo Fisher kit, even in the detection of SARS-CoV-2 mutations associated with its variants. This study reinforces the relevance of evaluating the performance of RT-qPCR kits before being used massively since those with lower sensitivity can generate false negatives and produce outbreaks of local infections. Hindawi 2022-07-05 /pmc/articles/PMC9259548/ /pubmed/35812012 http://dx.doi.org/10.1155/2022/2594564 Text en Copyright © 2022 Roberto Luraschi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Luraschi, Roberto Barrera-Avalos, Carlos Vallejos-Vidal, Eva Alarcón, Javiera Mella-Torres, Andrea Hernández, Felipe Inostroza-Molina, Ailen Valdés, Daniel Imarai, Mónica Acuña-Castillo, Claudio Reyes-López, Felipe E. Sandino, Ana María The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes |
title | The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes |
title_full | The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes |
title_fullStr | The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes |
title_full_unstemmed | The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes |
title_short | The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes |
title_sort | comparative analysis of two rt-qpcr kits for detecting sars-cov-2 reveals a higher risk of false-negative diagnosis in samples with high quantification cycles for viral and internal genes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259548/ https://www.ncbi.nlm.nih.gov/pubmed/35812012 http://dx.doi.org/10.1155/2022/2594564 |
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