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The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes

The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace the viral spread and the isolation of COVID-19-infected patients. The detection cap...

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Autores principales: Luraschi, Roberto, Barrera-Avalos, Carlos, Vallejos-Vidal, Eva, Alarcón, Javiera, Mella-Torres, Andrea, Hernández, Felipe, Inostroza-Molina, Ailen, Valdés, Daniel, Imarai, Mónica, Acuña-Castillo, Claudio, Reyes-López, Felipe E., Sandino, Ana María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259548/
https://www.ncbi.nlm.nih.gov/pubmed/35812012
http://dx.doi.org/10.1155/2022/2594564
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author Luraschi, Roberto
Barrera-Avalos, Carlos
Vallejos-Vidal, Eva
Alarcón, Javiera
Mella-Torres, Andrea
Hernández, Felipe
Inostroza-Molina, Ailen
Valdés, Daniel
Imarai, Mónica
Acuña-Castillo, Claudio
Reyes-López, Felipe E.
Sandino, Ana María
author_facet Luraschi, Roberto
Barrera-Avalos, Carlos
Vallejos-Vidal, Eva
Alarcón, Javiera
Mella-Torres, Andrea
Hernández, Felipe
Inostroza-Molina, Ailen
Valdés, Daniel
Imarai, Mónica
Acuña-Castillo, Claudio
Reyes-López, Felipe E.
Sandino, Ana María
author_sort Luraschi, Roberto
collection PubMed
description The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace the viral spread and the isolation of COVID-19-infected patients. The detection capacity of viral and internal genes is an essential parameter to consider and analyze during the assay. In this study, we analyze the performance of the two commercial RT-qPCR kits used in Chile, TaqMan™ 2019-nCoV Control Kit v1 (Thermo Fisher) and MaxCov19 (TAAG Genetics), for the COVID-19 diagnosis from nasopharyngeal swab samples (NPSs). Our results show a lower sensitivity of the TAAG kit compared to the Thermo Fisher kit, even in the detection of SARS-CoV-2 mutations associated with its variants. This study reinforces the relevance of evaluating the performance of RT-qPCR kits before being used massively since those with lower sensitivity can generate false negatives and produce outbreaks of local infections.
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spelling pubmed-92595482022-07-08 The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes Luraschi, Roberto Barrera-Avalos, Carlos Vallejos-Vidal, Eva Alarcón, Javiera Mella-Torres, Andrea Hernández, Felipe Inostroza-Molina, Ailen Valdés, Daniel Imarai, Mónica Acuña-Castillo, Claudio Reyes-López, Felipe E. Sandino, Ana María Can J Infect Dis Med Microbiol Research Article The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace the viral spread and the isolation of COVID-19-infected patients. The detection capacity of viral and internal genes is an essential parameter to consider and analyze during the assay. In this study, we analyze the performance of the two commercial RT-qPCR kits used in Chile, TaqMan™ 2019-nCoV Control Kit v1 (Thermo Fisher) and MaxCov19 (TAAG Genetics), for the COVID-19 diagnosis from nasopharyngeal swab samples (NPSs). Our results show a lower sensitivity of the TAAG kit compared to the Thermo Fisher kit, even in the detection of SARS-CoV-2 mutations associated with its variants. This study reinforces the relevance of evaluating the performance of RT-qPCR kits before being used massively since those with lower sensitivity can generate false negatives and produce outbreaks of local infections. Hindawi 2022-07-05 /pmc/articles/PMC9259548/ /pubmed/35812012 http://dx.doi.org/10.1155/2022/2594564 Text en Copyright © 2022 Roberto Luraschi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Luraschi, Roberto
Barrera-Avalos, Carlos
Vallejos-Vidal, Eva
Alarcón, Javiera
Mella-Torres, Andrea
Hernández, Felipe
Inostroza-Molina, Ailen
Valdés, Daniel
Imarai, Mónica
Acuña-Castillo, Claudio
Reyes-López, Felipe E.
Sandino, Ana María
The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes
title The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes
title_full The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes
title_fullStr The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes
title_full_unstemmed The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes
title_short The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes
title_sort comparative analysis of two rt-qpcr kits for detecting sars-cov-2 reveals a higher risk of false-negative diagnosis in samples with high quantification cycles for viral and internal genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259548/
https://www.ncbi.nlm.nih.gov/pubmed/35812012
http://dx.doi.org/10.1155/2022/2594564
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