Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration

The protozoan parasite Toxoplasma gondii (T. gondii) causes one of the most common human zoonotic diseases and infects approximately one-third of the global population. T. gondii infects nearly every cell type and causes severe symptoms in susceptible populations. In previous laboratory animal studi...

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Autores principales: Kim, Hyunho, Hong, Sung-Hee, Jeong, Hyo Eun, Han, Sewoon, Ahn, Jinchul, Kim, Jin-A., Yang, Ji-Hun, Oh, Hyun Jeong, Chung, Seok, Lee, Sang-Eun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259589/
https://www.ncbi.nlm.nih.gov/pubmed/35794197
http://dx.doi.org/10.1038/s41598-022-15305-4
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author Kim, Hyunho
Hong, Sung-Hee
Jeong, Hyo Eun
Han, Sewoon
Ahn, Jinchul
Kim, Jin-A.
Yang, Ji-Hun
Oh, Hyun Jeong
Chung, Seok
Lee, Sang-Eun
author_facet Kim, Hyunho
Hong, Sung-Hee
Jeong, Hyo Eun
Han, Sewoon
Ahn, Jinchul
Kim, Jin-A.
Yang, Ji-Hun
Oh, Hyun Jeong
Chung, Seok
Lee, Sang-Eun
author_sort Kim, Hyunho
collection PubMed
description The protozoan parasite Toxoplasma gondii (T. gondii) causes one of the most common human zoonotic diseases and infects approximately one-third of the global population. T. gondii infects nearly every cell type and causes severe symptoms in susceptible populations. In previous laboratory animal studies, T. gondii movement and transmission were not analyzed in real time. In a three-dimensional (3D) microfluidic assay, we successfully supported the complex lytic cycle of T. gondii in situ by generating a stable microvasculature. The physiology of the T. gondii-infected microvasculature was monitored in order to investigate the growth, paracellular and transcellular migration, and transmission of T. gondii, as well as the efficacy of T. gondii drugs.
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spelling pubmed-92595892022-07-08 Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration Kim, Hyunho Hong, Sung-Hee Jeong, Hyo Eun Han, Sewoon Ahn, Jinchul Kim, Jin-A. Yang, Ji-Hun Oh, Hyun Jeong Chung, Seok Lee, Sang-Eun Sci Rep Article The protozoan parasite Toxoplasma gondii (T. gondii) causes one of the most common human zoonotic diseases and infects approximately one-third of the global population. T. gondii infects nearly every cell type and causes severe symptoms in susceptible populations. In previous laboratory animal studies, T. gondii movement and transmission were not analyzed in real time. In a three-dimensional (3D) microfluidic assay, we successfully supported the complex lytic cycle of T. gondii in situ by generating a stable microvasculature. The physiology of the T. gondii-infected microvasculature was monitored in order to investigate the growth, paracellular and transcellular migration, and transmission of T. gondii, as well as the efficacy of T. gondii drugs. Nature Publishing Group UK 2022-07-06 /pmc/articles/PMC9259589/ /pubmed/35794197 http://dx.doi.org/10.1038/s41598-022-15305-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kim, Hyunho
Hong, Sung-Hee
Jeong, Hyo Eun
Han, Sewoon
Ahn, Jinchul
Kim, Jin-A.
Yang, Ji-Hun
Oh, Hyun Jeong
Chung, Seok
Lee, Sang-Eun
Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
title Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
title_full Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
title_fullStr Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
title_full_unstemmed Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
title_short Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
title_sort microfluidic model for in vitro acute toxoplasma gondii infection and transendothelial migration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259589/
https://www.ncbi.nlm.nih.gov/pubmed/35794197
http://dx.doi.org/10.1038/s41598-022-15305-4
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