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Safety and tolerability of intravitreal cetuximab in young and adult rabbits

To assess safety and tolerability of intraocularly applied cetuximab as epidermal growth factor receptor antibody, we conducted the experimental study which consisted of groups of adult rabbits (body weight: 2.4 kg) and young rabbits (body weight: 1.6 kg). All animals received three intravitreal inj...

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Autores principales: Bikbov, Mukharram M., Kazakbaeva, Gyulli M., Panda-Jonas, Songhomitra, Khakimov, Dinar A., Gilemzianova, Leisan I., Miniazeva, Liana A., Tuliakova, Azaliia M., Fakhretdinova, Albina A., Kazakbaev, Renat A., Nuriev, Ildar F., Jonas, Jost B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259608/
https://www.ncbi.nlm.nih.gov/pubmed/35794227
http://dx.doi.org/10.1038/s41598-022-15642-4
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author Bikbov, Mukharram M.
Kazakbaeva, Gyulli M.
Panda-Jonas, Songhomitra
Khakimov, Dinar A.
Gilemzianova, Leisan I.
Miniazeva, Liana A.
Tuliakova, Azaliia M.
Fakhretdinova, Albina A.
Kazakbaev, Renat A.
Nuriev, Ildar F.
Jonas, Jost B.
author_facet Bikbov, Mukharram M.
Kazakbaeva, Gyulli M.
Panda-Jonas, Songhomitra
Khakimov, Dinar A.
Gilemzianova, Leisan I.
Miniazeva, Liana A.
Tuliakova, Azaliia M.
Fakhretdinova, Albina A.
Kazakbaev, Renat A.
Nuriev, Ildar F.
Jonas, Jost B.
author_sort Bikbov, Mukharram M.
collection PubMed
description To assess safety and tolerability of intraocularly applied cetuximab as epidermal growth factor receptor antibody, we conducted the experimental study which consisted of groups of adult rabbits (body weight: 2.4 kg) and young rabbits (body weight: 1.6 kg). All animals received three intravitreal injections of 0.5 mg cetuximab (Erbitux) (0.10 mL; 5 mg cetuximab/mL) into their right eyes in 4-week intervals, while the contralateral eyes received intravitreal injections of Ringer's solution. All animals underwent regular ophthalmological examinations at baseline and two-week intervals. Four weeks after the last injection, the animals were sacrificed, and the eyes were enucleated, fixed and examined by light microscopy. The study included 10 adult rabbits (age: 18 weeks; range: 17–19 weeks) and 8 young rabbits (age: 8 weeks; range: 7–10 weeks). Biometric measurements of axial length, anterior chamber depth and lens thickness and intraocular pressure readings did not differ significantly (all P > 0.05, Bonferroni corrected) between the right (study) eyes and the left (control) eyes, neither in the young nor in the adult rabbit group. Signs of intraocular inflammation or fundus peculiarities were not detected. Thickness of the outer nuclear layer, inner nuclear layer, combined outer and inner nuclear layer and outer plexiform layer, and total retina, measured at the posterior pole, posterior pole/equator midpoint, equator, and ora serrata region, did not vary significantly between study eyes and control eyes (all P > 0.05, Bonferroni corrected). The results suggest that repeated intravitreal application of cetuximab did not result in any detected intraocular toxic or destructive effect in young and adult rabbits, concurring with the notion of an intraocular tolerability of cetuximab.
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spelling pubmed-92596082022-07-08 Safety and tolerability of intravitreal cetuximab in young and adult rabbits Bikbov, Mukharram M. Kazakbaeva, Gyulli M. Panda-Jonas, Songhomitra Khakimov, Dinar A. Gilemzianova, Leisan I. Miniazeva, Liana A. Tuliakova, Azaliia M. Fakhretdinova, Albina A. Kazakbaev, Renat A. Nuriev, Ildar F. Jonas, Jost B. Sci Rep Article To assess safety and tolerability of intraocularly applied cetuximab as epidermal growth factor receptor antibody, we conducted the experimental study which consisted of groups of adult rabbits (body weight: 2.4 kg) and young rabbits (body weight: 1.6 kg). All animals received three intravitreal injections of 0.5 mg cetuximab (Erbitux) (0.10 mL; 5 mg cetuximab/mL) into their right eyes in 4-week intervals, while the contralateral eyes received intravitreal injections of Ringer's solution. All animals underwent regular ophthalmological examinations at baseline and two-week intervals. Four weeks after the last injection, the animals were sacrificed, and the eyes were enucleated, fixed and examined by light microscopy. The study included 10 adult rabbits (age: 18 weeks; range: 17–19 weeks) and 8 young rabbits (age: 8 weeks; range: 7–10 weeks). Biometric measurements of axial length, anterior chamber depth and lens thickness and intraocular pressure readings did not differ significantly (all P > 0.05, Bonferroni corrected) between the right (study) eyes and the left (control) eyes, neither in the young nor in the adult rabbit group. Signs of intraocular inflammation or fundus peculiarities were not detected. Thickness of the outer nuclear layer, inner nuclear layer, combined outer and inner nuclear layer and outer plexiform layer, and total retina, measured at the posterior pole, posterior pole/equator midpoint, equator, and ora serrata region, did not vary significantly between study eyes and control eyes (all P > 0.05, Bonferroni corrected). The results suggest that repeated intravitreal application of cetuximab did not result in any detected intraocular toxic or destructive effect in young and adult rabbits, concurring with the notion of an intraocular tolerability of cetuximab. Nature Publishing Group UK 2022-07-06 /pmc/articles/PMC9259608/ /pubmed/35794227 http://dx.doi.org/10.1038/s41598-022-15642-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bikbov, Mukharram M.
Kazakbaeva, Gyulli M.
Panda-Jonas, Songhomitra
Khakimov, Dinar A.
Gilemzianova, Leisan I.
Miniazeva, Liana A.
Tuliakova, Azaliia M.
Fakhretdinova, Albina A.
Kazakbaev, Renat A.
Nuriev, Ildar F.
Jonas, Jost B.
Safety and tolerability of intravitreal cetuximab in young and adult rabbits
title Safety and tolerability of intravitreal cetuximab in young and adult rabbits
title_full Safety and tolerability of intravitreal cetuximab in young and adult rabbits
title_fullStr Safety and tolerability of intravitreal cetuximab in young and adult rabbits
title_full_unstemmed Safety and tolerability of intravitreal cetuximab in young and adult rabbits
title_short Safety and tolerability of intravitreal cetuximab in young and adult rabbits
title_sort safety and tolerability of intravitreal cetuximab in young and adult rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259608/
https://www.ncbi.nlm.nih.gov/pubmed/35794227
http://dx.doi.org/10.1038/s41598-022-15642-4
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