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High Intensity Concentric-Eccentric Exercise Under Hypoxia Changes the Blood Metabolome of Trained Athletes

The aim of this study was to determine alterations of the metabolome in blood plasma in response to concentric-eccentric leg exercise performed at a simulated altitude of 3,500 m. To do so, we recruited 11 well-trained subjects and performed an untargeted metabolomics analysis of plasma samples obta...

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Autores principales: Dünnwald, Tobias, Paglia, Giuseppe, Weiss, Günter, Denti, Vanna, Faulhaber, Martin, Schobersberger, Wolfgang, Wackerhage, Henning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260056/
https://www.ncbi.nlm.nih.gov/pubmed/35812339
http://dx.doi.org/10.3389/fphys.2022.904618
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author Dünnwald, Tobias
Paglia, Giuseppe
Weiss, Günter
Denti, Vanna
Faulhaber, Martin
Schobersberger, Wolfgang
Wackerhage, Henning
author_facet Dünnwald, Tobias
Paglia, Giuseppe
Weiss, Günter
Denti, Vanna
Faulhaber, Martin
Schobersberger, Wolfgang
Wackerhage, Henning
author_sort Dünnwald, Tobias
collection PubMed
description The aim of this study was to determine alterations of the metabolome in blood plasma in response to concentric-eccentric leg exercise performed at a simulated altitude of 3,500 m. To do so, we recruited 11 well-trained subjects and performed an untargeted metabolomics analysis of plasma samples obtained before, 20 min after as well as on day 8 after five sets of maximal, concentric-eccentric leg exercises that lasted 90 s each. We identified and annotated 115 metabolites through untargeted liquid chromatography-mass spectrometry metabolomics and used them to further calculate 20 sum/ratio of metabolites. A principal component analysis (PCA) revealed differences in-between the overall metabolome at rest and immediately after exercise. Interestingly, some systematic changes of relative metabolite concentrations still persisted on day 8 after exercise. The first two components of the PCA explained 34% of the relative concentrations of all identified metabolites analyzed together. A volcano plot indicates that 35 metabolites and two metabolite ratios were significantly changed directly after exercise, such as metabolites related to carbohydrate and TCA metabolism. Moreover, we observed alterations in the relative concentrations of amino acids (e.g., decreases of valine, leucine and increases in alanine) and purines (e.g., increases in hypoxanthine, xanthine and uric acid). In summary, high intensity concentric-eccentric exercise performed at simulated altitude systematically changed the blood metabolome in trained athletes directly after exercise and some relative metabolite concentrations were still changed on day 8. The importance of that persisting metabolic alterations on exercise performance should be studied further.
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spelling pubmed-92600562022-07-08 High Intensity Concentric-Eccentric Exercise Under Hypoxia Changes the Blood Metabolome of Trained Athletes Dünnwald, Tobias Paglia, Giuseppe Weiss, Günter Denti, Vanna Faulhaber, Martin Schobersberger, Wolfgang Wackerhage, Henning Front Physiol Physiology The aim of this study was to determine alterations of the metabolome in blood plasma in response to concentric-eccentric leg exercise performed at a simulated altitude of 3,500 m. To do so, we recruited 11 well-trained subjects and performed an untargeted metabolomics analysis of plasma samples obtained before, 20 min after as well as on day 8 after five sets of maximal, concentric-eccentric leg exercises that lasted 90 s each. We identified and annotated 115 metabolites through untargeted liquid chromatography-mass spectrometry metabolomics and used them to further calculate 20 sum/ratio of metabolites. A principal component analysis (PCA) revealed differences in-between the overall metabolome at rest and immediately after exercise. Interestingly, some systematic changes of relative metabolite concentrations still persisted on day 8 after exercise. The first two components of the PCA explained 34% of the relative concentrations of all identified metabolites analyzed together. A volcano plot indicates that 35 metabolites and two metabolite ratios were significantly changed directly after exercise, such as metabolites related to carbohydrate and TCA metabolism. Moreover, we observed alterations in the relative concentrations of amino acids (e.g., decreases of valine, leucine and increases in alanine) and purines (e.g., increases in hypoxanthine, xanthine and uric acid). In summary, high intensity concentric-eccentric exercise performed at simulated altitude systematically changed the blood metabolome in trained athletes directly after exercise and some relative metabolite concentrations were still changed on day 8. The importance of that persisting metabolic alterations on exercise performance should be studied further. Frontiers Media S.A. 2022-06-23 /pmc/articles/PMC9260056/ /pubmed/35812339 http://dx.doi.org/10.3389/fphys.2022.904618 Text en Copyright © 2022 Dünnwald, Paglia, Weiss, Denti, Faulhaber, Schobersberger and Wackerhage. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Dünnwald, Tobias
Paglia, Giuseppe
Weiss, Günter
Denti, Vanna
Faulhaber, Martin
Schobersberger, Wolfgang
Wackerhage, Henning
High Intensity Concentric-Eccentric Exercise Under Hypoxia Changes the Blood Metabolome of Trained Athletes
title High Intensity Concentric-Eccentric Exercise Under Hypoxia Changes the Blood Metabolome of Trained Athletes
title_full High Intensity Concentric-Eccentric Exercise Under Hypoxia Changes the Blood Metabolome of Trained Athletes
title_fullStr High Intensity Concentric-Eccentric Exercise Under Hypoxia Changes the Blood Metabolome of Trained Athletes
title_full_unstemmed High Intensity Concentric-Eccentric Exercise Under Hypoxia Changes the Blood Metabolome of Trained Athletes
title_short High Intensity Concentric-Eccentric Exercise Under Hypoxia Changes the Blood Metabolome of Trained Athletes
title_sort high intensity concentric-eccentric exercise under hypoxia changes the blood metabolome of trained athletes
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260056/
https://www.ncbi.nlm.nih.gov/pubmed/35812339
http://dx.doi.org/10.3389/fphys.2022.904618
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