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A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials
Frequent meat frauds have become a global issue because adulteration risks the food safety, breaches market rules, and even threatens public health. Multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for the identification of meat products in food industr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260169/ https://www.ncbi.nlm.nih.gov/pubmed/35811966 http://dx.doi.org/10.3389/fnut.2022.890537 |
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author | Zhou, Song Zhong, Guowei Zhou, Hanxiao Zhang, Xiaoxia Zeng, Xiaoqun Wu, Zhen Pan, Daodong He, Jun Cai, Zhendong Liu, Qianqian |
author_facet | Zhou, Song Zhong, Guowei Zhou, Hanxiao Zhang, Xiaoxia Zeng, Xiaoqun Wu, Zhen Pan, Daodong He, Jun Cai, Zhendong Liu, Qianqian |
author_sort | Zhou, Song |
collection | PubMed |
description | Frequent meat frauds have become a global issue because adulteration risks the food safety, breaches market rules, and even threatens public health. Multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for the identification of meat products in food industries. However, relatively less is known about a multiplex PCR method authenticating seven animal species simultaneously in one reaction due to technological challenge. Through screening new species-specific primers and optimizing PCR system, a heptaplex PCR method was established, which could simultaneously detect seven meat ingredients of camel (128 bp), pigeon (157 bp), chicken (220 bp), duck (272 bp), horse (314 bp), beef (434 bp), and pork (502 bp) in a single-tube reaction. DNA sequencing solidly validated that each set of primers specifically amplified target species from total DNA mixtures of seven meat species. The developed multiplex assay was stable and sensitive enough to detect 0.01–0.025 ng DNA from various meat treatments including raw, boiled, and autoclaved meat samples or target meat content of 0.1% total meat weight, suggesting the suitability of the heptaplex PCR technique for tracing target meats with high accuracy and precision. Most importantly, a market survey validated the availability of this multiplex PCR technique in real-world meat products with a good application foreground. |
format | Online Article Text |
id | pubmed-9260169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92601692022-07-08 A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials Zhou, Song Zhong, Guowei Zhou, Hanxiao Zhang, Xiaoxia Zeng, Xiaoqun Wu, Zhen Pan, Daodong He, Jun Cai, Zhendong Liu, Qianqian Front Nutr Nutrition Frequent meat frauds have become a global issue because adulteration risks the food safety, breaches market rules, and even threatens public health. Multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for the identification of meat products in food industries. However, relatively less is known about a multiplex PCR method authenticating seven animal species simultaneously in one reaction due to technological challenge. Through screening new species-specific primers and optimizing PCR system, a heptaplex PCR method was established, which could simultaneously detect seven meat ingredients of camel (128 bp), pigeon (157 bp), chicken (220 bp), duck (272 bp), horse (314 bp), beef (434 bp), and pork (502 bp) in a single-tube reaction. DNA sequencing solidly validated that each set of primers specifically amplified target species from total DNA mixtures of seven meat species. The developed multiplex assay was stable and sensitive enough to detect 0.01–0.025 ng DNA from various meat treatments including raw, boiled, and autoclaved meat samples or target meat content of 0.1% total meat weight, suggesting the suitability of the heptaplex PCR technique for tracing target meats with high accuracy and precision. Most importantly, a market survey validated the availability of this multiplex PCR technique in real-world meat products with a good application foreground. Frontiers Media S.A. 2022-06-23 /pmc/articles/PMC9260169/ /pubmed/35811966 http://dx.doi.org/10.3389/fnut.2022.890537 Text en Copyright © 2022 Zhou, Zhong, Zhou, Zhang, Zeng, Wu, Pan, He, Cai and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Zhou, Song Zhong, Guowei Zhou, Hanxiao Zhang, Xiaoxia Zeng, Xiaoqun Wu, Zhen Pan, Daodong He, Jun Cai, Zhendong Liu, Qianqian A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials |
title | A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials |
title_full | A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials |
title_fullStr | A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials |
title_full_unstemmed | A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials |
title_short | A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials |
title_sort | heptaplex pcr assay for molecular traceability of species origin with high efficiency and practicality in both raw and heat processing meat materials |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260169/ https://www.ncbi.nlm.nih.gov/pubmed/35811966 http://dx.doi.org/10.3389/fnut.2022.890537 |
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