Cargando…

A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials

Frequent meat frauds have become a global issue because adulteration risks the food safety, breaches market rules, and even threatens public health. Multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for the identification of meat products in food industr...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Song, Zhong, Guowei, Zhou, Hanxiao, Zhang, Xiaoxia, Zeng, Xiaoqun, Wu, Zhen, Pan, Daodong, He, Jun, Cai, Zhendong, Liu, Qianqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260169/
https://www.ncbi.nlm.nih.gov/pubmed/35811966
http://dx.doi.org/10.3389/fnut.2022.890537
_version_ 1784741961710698496
author Zhou, Song
Zhong, Guowei
Zhou, Hanxiao
Zhang, Xiaoxia
Zeng, Xiaoqun
Wu, Zhen
Pan, Daodong
He, Jun
Cai, Zhendong
Liu, Qianqian
author_facet Zhou, Song
Zhong, Guowei
Zhou, Hanxiao
Zhang, Xiaoxia
Zeng, Xiaoqun
Wu, Zhen
Pan, Daodong
He, Jun
Cai, Zhendong
Liu, Qianqian
author_sort Zhou, Song
collection PubMed
description Frequent meat frauds have become a global issue because adulteration risks the food safety, breaches market rules, and even threatens public health. Multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for the identification of meat products in food industries. However, relatively less is known about a multiplex PCR method authenticating seven animal species simultaneously in one reaction due to technological challenge. Through screening new species-specific primers and optimizing PCR system, a heptaplex PCR method was established, which could simultaneously detect seven meat ingredients of camel (128 bp), pigeon (157 bp), chicken (220 bp), duck (272 bp), horse (314 bp), beef (434 bp), and pork (502 bp) in a single-tube reaction. DNA sequencing solidly validated that each set of primers specifically amplified target species from total DNA mixtures of seven meat species. The developed multiplex assay was stable and sensitive enough to detect 0.01–0.025 ng DNA from various meat treatments including raw, boiled, and autoclaved meat samples or target meat content of 0.1% total meat weight, suggesting the suitability of the heptaplex PCR technique for tracing target meats with high accuracy and precision. Most importantly, a market survey validated the availability of this multiplex PCR technique in real-world meat products with a good application foreground.
format Online
Article
Text
id pubmed-9260169
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92601692022-07-08 A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials Zhou, Song Zhong, Guowei Zhou, Hanxiao Zhang, Xiaoxia Zeng, Xiaoqun Wu, Zhen Pan, Daodong He, Jun Cai, Zhendong Liu, Qianqian Front Nutr Nutrition Frequent meat frauds have become a global issue because adulteration risks the food safety, breaches market rules, and even threatens public health. Multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for the identification of meat products in food industries. However, relatively less is known about a multiplex PCR method authenticating seven animal species simultaneously in one reaction due to technological challenge. Through screening new species-specific primers and optimizing PCR system, a heptaplex PCR method was established, which could simultaneously detect seven meat ingredients of camel (128 bp), pigeon (157 bp), chicken (220 bp), duck (272 bp), horse (314 bp), beef (434 bp), and pork (502 bp) in a single-tube reaction. DNA sequencing solidly validated that each set of primers specifically amplified target species from total DNA mixtures of seven meat species. The developed multiplex assay was stable and sensitive enough to detect 0.01–0.025 ng DNA from various meat treatments including raw, boiled, and autoclaved meat samples or target meat content of 0.1% total meat weight, suggesting the suitability of the heptaplex PCR technique for tracing target meats with high accuracy and precision. Most importantly, a market survey validated the availability of this multiplex PCR technique in real-world meat products with a good application foreground. Frontiers Media S.A. 2022-06-23 /pmc/articles/PMC9260169/ /pubmed/35811966 http://dx.doi.org/10.3389/fnut.2022.890537 Text en Copyright © 2022 Zhou, Zhong, Zhou, Zhang, Zeng, Wu, Pan, He, Cai and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Zhou, Song
Zhong, Guowei
Zhou, Hanxiao
Zhang, Xiaoxia
Zeng, Xiaoqun
Wu, Zhen
Pan, Daodong
He, Jun
Cai, Zhendong
Liu, Qianqian
A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials
title A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials
title_full A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials
title_fullStr A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials
title_full_unstemmed A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials
title_short A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials
title_sort heptaplex pcr assay for molecular traceability of species origin with high efficiency and practicality in both raw and heat processing meat materials
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260169/
https://www.ncbi.nlm.nih.gov/pubmed/35811966
http://dx.doi.org/10.3389/fnut.2022.890537
work_keys_str_mv AT zhousong aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zhongguowei aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zhouhanxiao aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zhangxiaoxia aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zengxiaoqun aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT wuzhen aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT pandaodong aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT hejun aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT caizhendong aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT liuqianqian aheptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zhousong heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zhongguowei heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zhouhanxiao heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zhangxiaoxia heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT zengxiaoqun heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT wuzhen heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT pandaodong heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT hejun heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT caizhendong heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials
AT liuqianqian heptaplexpcrassayformoleculartraceabilityofspeciesoriginwithhighefficiencyandpracticalityinbothrawandheatprocessingmeatmaterials