Research Note: Methods in detecting signs of life after gaseous stun in broilers

To be compliant with religious requirements for poultry, slaughter facilities using controlled atmosphere stunning may require a reliable method for detecting signs of life. However, the production setting challenges methods utilized in veterinary and field use by generating excess sound and vibration, which can impact the effectiveness of typical measurement tools, such as the stethoscope or doppler ultrasound. In addition, it is required that the process be quick and efficient as to not interfere with the rapid pace of production. The current study assessed various methods including reflexes (pupillary light reflex, nictitating membrane reflex, and pedal reflex), doppler ultrasound, stethoscopes, and pulse oximeter for determination of signs of life in broiler chickens postcontrolled atmosphere stunning in production. Data were analyzed using a generalized linear mixed model in SAS. Each bird was considered an experimental unit (n = 18) and differences between dead on arrival (DOA; n = 6), stunned (n = 10) and sensible (n = 2) birds were compared using contrast statements. Reflexes were seen only in sensible birds. Of the evaluated tools, only the pulse oximeter was consistently capable of detecting heart beats per minute (bpm) within the production environment. Although the values of bpm did not differ between sensible and stunned birds (P = 0.724), DOA birds had a lower bpm than all others (P < 0.001). Although the accuracy of results deserves further investigation and the small sample size of this study posed challenges to our statistical analyses, including low statistical power for the comparison between sensible and stunned groups, the achieved results suggest that the pulse oximeter meets the requirements for a reliable and practical method in detecting signs of life in broilers. With the increasing importance of halal products in a market that prioritizes animal welfare, our results present a promising approach to meet halal certification requirements. Further studies on this topic are strongly encouraged.