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Modulation of T Cell Responses by Fucoidan to Inhibit Osteogenesis

Fucoidan has sparked considerable interest in biomedical applications because of its inherent (bio)physicochemical characteristics, particularly immunomodulatory effects on macrophages, neutrophils, and natural killer cells. However, the effect of fucoidan on T cells and the following regulatory int...

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Autores principales: Huang, Hailin, Guo, Fangze, Deng, Xuyang, Yan, Mingzhe, Wang, Danyang, Sun, Zhanyi, Yuan, Changqing, Zhou, Qihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260855/
https://www.ncbi.nlm.nih.gov/pubmed/35812368
http://dx.doi.org/10.3389/fimmu.2022.911390
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author Huang, Hailin
Guo, Fangze
Deng, Xuyang
Yan, Mingzhe
Wang, Danyang
Sun, Zhanyi
Yuan, Changqing
Zhou, Qihui
author_facet Huang, Hailin
Guo, Fangze
Deng, Xuyang
Yan, Mingzhe
Wang, Danyang
Sun, Zhanyi
Yuan, Changqing
Zhou, Qihui
author_sort Huang, Hailin
collection PubMed
description Fucoidan has sparked considerable interest in biomedical applications because of its inherent (bio)physicochemical characteristics, particularly immunomodulatory effects on macrophages, neutrophils, and natural killer cells. However, the effect of fucoidan on T cells and the following regulatory interaction on cellular function has not been reported. In this work, the effect of sterile fucoidan on the T-cell response and the subsequent modulation of osteogenesis is investigated. The physicochemical features of fucoidan treated by high-temperature autoclave sterilization are characterized by UV–visible spectroscopy, X-ray diffraction, Fourier transform infrared and nuclear magnetic resonance analysis. It is demonstrated that high-temperature autoclave treatment resulted in fucoidan depolymerization, with no change in its key bioactive groups. Further, sterile fucoidan promotes T cells proliferation and the proportion of differentiated T cells decreases with increasing concentration of fucoidan. In addition, the supernatant of T cells co-cultured with fucoidan greatly suppresses the osteogenic differentiation of MC3T3-E1 by downregulating the formation of alkaline phosphatase and calcium nodule compared with fucoidan. Therefore, our work offers new insight into the fucoidan-mediated T cell and osteoblast interplay.
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spelling pubmed-92608552022-07-08 Modulation of T Cell Responses by Fucoidan to Inhibit Osteogenesis Huang, Hailin Guo, Fangze Deng, Xuyang Yan, Mingzhe Wang, Danyang Sun, Zhanyi Yuan, Changqing Zhou, Qihui Front Immunol Immunology Fucoidan has sparked considerable interest in biomedical applications because of its inherent (bio)physicochemical characteristics, particularly immunomodulatory effects on macrophages, neutrophils, and natural killer cells. However, the effect of fucoidan on T cells and the following regulatory interaction on cellular function has not been reported. In this work, the effect of sterile fucoidan on the T-cell response and the subsequent modulation of osteogenesis is investigated. The physicochemical features of fucoidan treated by high-temperature autoclave sterilization are characterized by UV–visible spectroscopy, X-ray diffraction, Fourier transform infrared and nuclear magnetic resonance analysis. It is demonstrated that high-temperature autoclave treatment resulted in fucoidan depolymerization, with no change in its key bioactive groups. Further, sterile fucoidan promotes T cells proliferation and the proportion of differentiated T cells decreases with increasing concentration of fucoidan. In addition, the supernatant of T cells co-cultured with fucoidan greatly suppresses the osteogenic differentiation of MC3T3-E1 by downregulating the formation of alkaline phosphatase and calcium nodule compared with fucoidan. Therefore, our work offers new insight into the fucoidan-mediated T cell and osteoblast interplay. Frontiers Media S.A. 2022-06-23 /pmc/articles/PMC9260855/ /pubmed/35812368 http://dx.doi.org/10.3389/fimmu.2022.911390 Text en Copyright © 2022 Huang, Guo, Deng, Yan, Wang, Sun, Yuan and Zhou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Huang, Hailin
Guo, Fangze
Deng, Xuyang
Yan, Mingzhe
Wang, Danyang
Sun, Zhanyi
Yuan, Changqing
Zhou, Qihui
Modulation of T Cell Responses by Fucoidan to Inhibit Osteogenesis
title Modulation of T Cell Responses by Fucoidan to Inhibit Osteogenesis
title_full Modulation of T Cell Responses by Fucoidan to Inhibit Osteogenesis
title_fullStr Modulation of T Cell Responses by Fucoidan to Inhibit Osteogenesis
title_full_unstemmed Modulation of T Cell Responses by Fucoidan to Inhibit Osteogenesis
title_short Modulation of T Cell Responses by Fucoidan to Inhibit Osteogenesis
title_sort modulation of t cell responses by fucoidan to inhibit osteogenesis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260855/
https://www.ncbi.nlm.nih.gov/pubmed/35812368
http://dx.doi.org/10.3389/fimmu.2022.911390
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