Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants

Isatis indigotica Fort. (family Cruciferae), is an herb widely used in traditional herbal medicine and its dried leave was named “ISATIDIS FOLIUM”. Baphicacanthus cusia (Ness) Bremek. and Polygonum tinctorium Ait. are commonly misused as ISATIDIS FOLIUM in Chinese Medicine pharmacy. For the purpose...

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Autores principales: Hsieh, Yung-Chuan, Wu, Ming-Sian, Lee, Hui-Chun, Hsieh, Chia-Yun, Huang, Shih-Shan, Tsai, Chia-Fen, Lin, Ya-Tze, Lin, Mei-Chih, Tseng, Su-Hsiang, Wang, Der-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261822/
https://www.ncbi.nlm.nih.gov/pubmed/35696207
http://dx.doi.org/10.38212/2224-6614.3354
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author Hsieh, Yung-Chuan
Wu, Ming-Sian
Lee, Hui-Chun
Hsieh, Chia-Yun
Huang, Shih-Shan
Tsai, Chia-Fen
Lin, Ya-Tze
Lin, Mei-Chih
Tseng, Su-Hsiang
Wang, Der-Yuan
author_facet Hsieh, Yung-Chuan
Wu, Ming-Sian
Lee, Hui-Chun
Hsieh, Chia-Yun
Huang, Shih-Shan
Tsai, Chia-Fen
Lin, Ya-Tze
Lin, Mei-Chih
Tseng, Su-Hsiang
Wang, Der-Yuan
author_sort Hsieh, Yung-Chuan
collection PubMed
description Isatis indigotica Fort. (family Cruciferae), is an herb widely used in traditional herbal medicine and its dried leave was named “ISATIDIS FOLIUM”. Baphicacanthus cusia (Ness) Bremek. and Polygonum tinctorium Ait. are commonly misused as ISATIDIS FOLIUM in Chinese Medicine pharmacy. For the purpose of being not misused, specific primers based on the sequence difference of chloroplast trnH-psbA intergenic spacer were designed and multiplex polymerase chain reaction method (multiplex PCR) was developed. In this study, 29 original herbal materials were analyzed and our results show that DNA size after multiplex PCR was able to distinguish variations between three herbs. DNA fragments of 464, 297, 170 base pairs (bps) were represented for I. indigotica and B. cusia and P. tinctorium, respectively. In conclusion, our investigations demonstrate that molecular identification method provides more accurate results for medicinal plants detection and good quality control of ISATIDIS FOLIUM.
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spelling pubmed-92618222022-07-18 Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants Hsieh, Yung-Chuan Wu, Ming-Sian Lee, Hui-Chun Hsieh, Chia-Yun Huang, Shih-Shan Tsai, Chia-Fen Lin, Ya-Tze Lin, Mei-Chih Tseng, Su-Hsiang Wang, Der-Yuan J Food Drug Anal Original Article Isatis indigotica Fort. (family Cruciferae), is an herb widely used in traditional herbal medicine and its dried leave was named “ISATIDIS FOLIUM”. Baphicacanthus cusia (Ness) Bremek. and Polygonum tinctorium Ait. are commonly misused as ISATIDIS FOLIUM in Chinese Medicine pharmacy. For the purpose of being not misused, specific primers based on the sequence difference of chloroplast trnH-psbA intergenic spacer were designed and multiplex polymerase chain reaction method (multiplex PCR) was developed. In this study, 29 original herbal materials were analyzed and our results show that DNA size after multiplex PCR was able to distinguish variations between three herbs. DNA fragments of 464, 297, 170 base pairs (bps) were represented for I. indigotica and B. cusia and P. tinctorium, respectively. In conclusion, our investigations demonstrate that molecular identification method provides more accurate results for medicinal plants detection and good quality control of ISATIDIS FOLIUM. Taiwan Food and Drug Administration 2021-06-15 /pmc/articles/PMC9261822/ /pubmed/35696207 http://dx.doi.org/10.38212/2224-6614.3354 Text en © 2021 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Hsieh, Yung-Chuan
Wu, Ming-Sian
Lee, Hui-Chun
Hsieh, Chia-Yun
Huang, Shih-Shan
Tsai, Chia-Fen
Lin, Ya-Tze
Lin, Mei-Chih
Tseng, Su-Hsiang
Wang, Der-Yuan
Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants
title Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants
title_full Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants
title_fullStr Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants
title_full_unstemmed Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants
title_short Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants
title_sort development of the species-specific multiplex pcr and dna sequencing methods for rapid authentication of isatidis folium and its adulterants
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261822/
https://www.ncbi.nlm.nih.gov/pubmed/35696207
http://dx.doi.org/10.38212/2224-6614.3354
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