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UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L.
Accurate assays of plant antioxidants and other phytochemicals require efficient extraction conditions and enable rigorous assessments of crop varieties and production systems. This study assessed the extraction of phytochemicals and antioxidants from conventionally or organically grown red and gold...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taiwan Food and Drug Administration
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261854/ https://www.ncbi.nlm.nih.gov/pubmed/35696117 http://dx.doi.org/10.38212/2224-6614.1056 |
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author | Wang, Junyi Jayaprakasha, Guddadarangavvanahally Krishnareddy Patil, Bhimanagouda S. |
author_facet | Wang, Junyi Jayaprakasha, Guddadarangavvanahally Krishnareddy Patil, Bhimanagouda S. |
author_sort | Wang, Junyi |
collection | PubMed |
description | Accurate assays of plant antioxidants and other phytochemicals require efficient extraction conditions and enable rigorous assessments of crop varieties and production systems. This study assessed the extraction of phytochemicals and antioxidants from conventionally or organically grown red and golden beets (Beta vulgaris L.), using twenty solvent (S1–S20) mixtures containing water, methanol, and ethanol alone or with acids (ascorbic, formic, acetic). Red beetroot extracted with methanol with or without acid had the highest betanin content (2791.0 μg/g and 8222.3 μg/g of fresh weight [FW], respectively) and golden beetroot extracted with methanol/ascorbic acid/water had the highest vulgaxanthin I (193.7 μg/g and 15.0 μg/g of FW, respectively). The radical-scavenging activity and total phenolics in beetroot extracts reflected the different extraction efficiency of each solvent. UHPLC-QTOF-MS was used to identify twenty-seven phytochemicals, including 23 betalains, 2 amino acids, and 2 phenolic acids. Chemometric approaches discriminated the beet varieties and different extracts within one variety based on the composition and abundance of the key phytochemicals. The red beetroot extracted with aqueous ethanol with or without acid (S5, S7, S8, S9), and golden beetroot extracted with methanol-containing solvents (S15 for conventionally and S20 for organically) had the highest levels of phytochemicals, suggesting that these conditions efficiently extract key phytochemicals. |
format | Online Article Text |
id | pubmed-9261854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taiwan Food and Drug Administration |
record_format | MEDLINE/PubMed |
spelling | pubmed-92618542022-07-18 UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L. Wang, Junyi Jayaprakasha, Guddadarangavvanahally Krishnareddy Patil, Bhimanagouda S. J Food Drug Anal Original Article Accurate assays of plant antioxidants and other phytochemicals require efficient extraction conditions and enable rigorous assessments of crop varieties and production systems. This study assessed the extraction of phytochemicals and antioxidants from conventionally or organically grown red and golden beets (Beta vulgaris L.), using twenty solvent (S1–S20) mixtures containing water, methanol, and ethanol alone or with acids (ascorbic, formic, acetic). Red beetroot extracted with methanol with or without acid had the highest betanin content (2791.0 μg/g and 8222.3 μg/g of fresh weight [FW], respectively) and golden beetroot extracted with methanol/ascorbic acid/water had the highest vulgaxanthin I (193.7 μg/g and 15.0 μg/g of FW, respectively). The radical-scavenging activity and total phenolics in beetroot extracts reflected the different extraction efficiency of each solvent. UHPLC-QTOF-MS was used to identify twenty-seven phytochemicals, including 23 betalains, 2 amino acids, and 2 phenolic acids. Chemometric approaches discriminated the beet varieties and different extracts within one variety based on the composition and abundance of the key phytochemicals. The red beetroot extracted with aqueous ethanol with or without acid (S5, S7, S8, S9), and golden beetroot extracted with methanol-containing solvents (S15 for conventionally and S20 for organically) had the highest levels of phytochemicals, suggesting that these conditions efficiently extract key phytochemicals. Taiwan Food and Drug Administration 2020-06-27 /pmc/articles/PMC9261854/ /pubmed/35696117 http://dx.doi.org/10.38212/2224-6614.1056 Text en © 2020 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Original Article Wang, Junyi Jayaprakasha, Guddadarangavvanahally Krishnareddy Patil, Bhimanagouda S. UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L. |
title | UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L. |
title_full | UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L. |
title_fullStr | UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L. |
title_full_unstemmed | UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L. |
title_short | UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L. |
title_sort | uplc-qtof-ms fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of beta vulgaris l. |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261854/ https://www.ncbi.nlm.nih.gov/pubmed/35696117 http://dx.doi.org/10.38212/2224-6614.1056 |
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