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Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard

Non-avian reptiles, unlike mammals and birds, have undergone numerous sex determination changes. Casque-Headed Lizards have replaced the ancestral XY system shared across pleurodonts with a new pair of XY chromosomes. However, the evolutionary forces that triggered this transition have remained uncl...

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Autores principales: Suárez-Varón, Gabriel, Mendoza-Cruz, Eva, Acosta, Armando, Villagrán-Santa Cruz, Maricela, Cortez, Diego, Hernández-Gallegos, Oswaldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262179/
https://www.ncbi.nlm.nih.gov/pubmed/35797377
http://dx.doi.org/10.1371/journal.pone.0263804
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author Suárez-Varón, Gabriel
Mendoza-Cruz, Eva
Acosta, Armando
Villagrán-Santa Cruz, Maricela
Cortez, Diego
Hernández-Gallegos, Oswaldo
author_facet Suárez-Varón, Gabriel
Mendoza-Cruz, Eva
Acosta, Armando
Villagrán-Santa Cruz, Maricela
Cortez, Diego
Hernández-Gallegos, Oswaldo
author_sort Suárez-Varón, Gabriel
collection PubMed
description Non-avian reptiles, unlike mammals and birds, have undergone numerous sex determination changes. Casque-Headed Lizards have replaced the ancestral XY system shared across pleurodonts with a new pair of XY chromosomes. However, the evolutionary forces that triggered this transition have remained unclear. An interesting hypothesis suggests that species with intermediate states, with sex chromosomes but also thermal-induced sex reversal at specific incubation temperatures, could be more susceptible to sex determination turnovers. We contrasted genotypic data (presence/absence of the Y chromosome) against the histology of gonads of embryos from stages 35–37 incubated at various temperatures, including typical male-producing (26°C) and female-producing (32°C) temperatures. Our work apparently reports for the first time the histology of gonads, including morphological changes, from stages 35–37 of development in the family Corytophanidae. We also observed that all embryos developed hemipenes, suggesting sex-linked developmental heterochrony. We observed perfect concordance between genotype and phenotype at all temperatures. However, analysis of transcriptomic data from embryos incubated at 26°C and 32°C identified transcript variants of the chromatin modifiers JARID2 and KDM6B that have been linked to temperature-dependent sex determination in other reptiles. Our work tested the validity of a mixed sex determination system in the family Corytophanidae. We found that XY chromosomes are dominant; however, our work supports the hypothesis of a conserved transcriptional response to incubation temperatures across non-avian reptiles that could be a reminiscence of an ancestral sex determination system.
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spelling pubmed-92621792022-07-08 Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard Suárez-Varón, Gabriel Mendoza-Cruz, Eva Acosta, Armando Villagrán-Santa Cruz, Maricela Cortez, Diego Hernández-Gallegos, Oswaldo PLoS One Research Article Non-avian reptiles, unlike mammals and birds, have undergone numerous sex determination changes. Casque-Headed Lizards have replaced the ancestral XY system shared across pleurodonts with a new pair of XY chromosomes. However, the evolutionary forces that triggered this transition have remained unclear. An interesting hypothesis suggests that species with intermediate states, with sex chromosomes but also thermal-induced sex reversal at specific incubation temperatures, could be more susceptible to sex determination turnovers. We contrasted genotypic data (presence/absence of the Y chromosome) against the histology of gonads of embryos from stages 35–37 incubated at various temperatures, including typical male-producing (26°C) and female-producing (32°C) temperatures. Our work apparently reports for the first time the histology of gonads, including morphological changes, from stages 35–37 of development in the family Corytophanidae. We also observed that all embryos developed hemipenes, suggesting sex-linked developmental heterochrony. We observed perfect concordance between genotype and phenotype at all temperatures. However, analysis of transcriptomic data from embryos incubated at 26°C and 32°C identified transcript variants of the chromatin modifiers JARID2 and KDM6B that have been linked to temperature-dependent sex determination in other reptiles. Our work tested the validity of a mixed sex determination system in the family Corytophanidae. We found that XY chromosomes are dominant; however, our work supports the hypothesis of a conserved transcriptional response to incubation temperatures across non-avian reptiles that could be a reminiscence of an ancestral sex determination system. Public Library of Science 2022-07-07 /pmc/articles/PMC9262179/ /pubmed/35797377 http://dx.doi.org/10.1371/journal.pone.0263804 Text en © 2022 Suárez-Varón et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Suárez-Varón, Gabriel
Mendoza-Cruz, Eva
Acosta, Armando
Villagrán-Santa Cruz, Maricela
Cortez, Diego
Hernández-Gallegos, Oswaldo
Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard
title Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard
title_full Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard
title_fullStr Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard
title_full_unstemmed Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard
title_short Genetic determination and JARID2 over-expression in a thermal incubation experiment in Casque-Headed Lizard
title_sort genetic determination and jarid2 over-expression in a thermal incubation experiment in casque-headed lizard
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262179/
https://www.ncbi.nlm.nih.gov/pubmed/35797377
http://dx.doi.org/10.1371/journal.pone.0263804
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