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Intramammary infections with Corynebacterium spp. in bovine lactating udder quarters

Corynebacterium spp. are frequently detected in bovine quarter milk samples, yet their impact on udder health has not been determined completely. In this longitudinal study, we collected quarter milk samples from a dairy herd of approximately 200 cows, ten times at 14 d intervals. Bacteriologically,...

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Autores principales: Lücken, Anneke, Woudstra, Svenja, Wente, Nicole, Zhang, Yanchao, Krömker, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262192/
https://www.ncbi.nlm.nih.gov/pubmed/35797266
http://dx.doi.org/10.1371/journal.pone.0270867
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author Lücken, Anneke
Woudstra, Svenja
Wente, Nicole
Zhang, Yanchao
Krömker, Volker
author_facet Lücken, Anneke
Woudstra, Svenja
Wente, Nicole
Zhang, Yanchao
Krömker, Volker
author_sort Lücken, Anneke
collection PubMed
description Corynebacterium spp. are frequently detected in bovine quarter milk samples, yet their impact on udder health has not been determined completely. In this longitudinal study, we collected quarter milk samples from a dairy herd of approximately 200 cows, ten times at 14 d intervals. Bacteriologically, Catalase-positive and Gram-positive rods were detected in 22.7% of the samples. For further species diagnosis, colonies were analyzed by MALDI-TOF MS. Corynebacterium bovis, C. amycolatum, C. xerosis and 10 other Corynebacterium spp. were detected. The three aforementioned species accounted for 88.4%, 8.65% and 0.94% of all cultured Corynebacterium spp., respectively. For further evaluation of infection dynamics, the following three infection definitions were applied: A (2/3 consecutive samples positive for the same species), B (≥1000 cfu/mL in one sample), C (isolated from a clinical mastitis case). Infections according to definition B occurred most frequently and clinical mastitis with Corynebacterium spp. occurred once during sampling. Life tables were used to determine the duration of infection. According to infection definition A, infection durations of 111 d and 98 d were obtained for C. bovis and C. amycolatum, respectively. Exemplarily, longer lasting infections were examined for their strain diversity by RAPD PCR. A low strain diversity was found in the individual quarters that indicates a longer colonization of the udder parenchyma by C. bovis and C. amycolatum.
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spelling pubmed-92621922022-07-08 Intramammary infections with Corynebacterium spp. in bovine lactating udder quarters Lücken, Anneke Woudstra, Svenja Wente, Nicole Zhang, Yanchao Krömker, Volker PLoS One Research Article Corynebacterium spp. are frequently detected in bovine quarter milk samples, yet their impact on udder health has not been determined completely. In this longitudinal study, we collected quarter milk samples from a dairy herd of approximately 200 cows, ten times at 14 d intervals. Bacteriologically, Catalase-positive and Gram-positive rods were detected in 22.7% of the samples. For further species diagnosis, colonies were analyzed by MALDI-TOF MS. Corynebacterium bovis, C. amycolatum, C. xerosis and 10 other Corynebacterium spp. were detected. The three aforementioned species accounted for 88.4%, 8.65% and 0.94% of all cultured Corynebacterium spp., respectively. For further evaluation of infection dynamics, the following three infection definitions were applied: A (2/3 consecutive samples positive for the same species), B (≥1000 cfu/mL in one sample), C (isolated from a clinical mastitis case). Infections according to definition B occurred most frequently and clinical mastitis with Corynebacterium spp. occurred once during sampling. Life tables were used to determine the duration of infection. According to infection definition A, infection durations of 111 d and 98 d were obtained for C. bovis and C. amycolatum, respectively. Exemplarily, longer lasting infections were examined for their strain diversity by RAPD PCR. A low strain diversity was found in the individual quarters that indicates a longer colonization of the udder parenchyma by C. bovis and C. amycolatum. Public Library of Science 2022-07-07 /pmc/articles/PMC9262192/ /pubmed/35797266 http://dx.doi.org/10.1371/journal.pone.0270867 Text en © 2022 Lücken et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lücken, Anneke
Woudstra, Svenja
Wente, Nicole
Zhang, Yanchao
Krömker, Volker
Intramammary infections with Corynebacterium spp. in bovine lactating udder quarters
title Intramammary infections with Corynebacterium spp. in bovine lactating udder quarters
title_full Intramammary infections with Corynebacterium spp. in bovine lactating udder quarters
title_fullStr Intramammary infections with Corynebacterium spp. in bovine lactating udder quarters
title_full_unstemmed Intramammary infections with Corynebacterium spp. in bovine lactating udder quarters
title_short Intramammary infections with Corynebacterium spp. in bovine lactating udder quarters
title_sort intramammary infections with corynebacterium spp. in bovine lactating udder quarters
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262192/
https://www.ncbi.nlm.nih.gov/pubmed/35797266
http://dx.doi.org/10.1371/journal.pone.0270867
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