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A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors

Genome editing in human induced pluripotent stem cells (hiPSCs) offers a potential tool for studying gene functions in disease models and correcting genetic mutations for cell-based therapy. Precise transgene insertion in hiPSCs represents a significant challenge. In the past decade, viral transduct...

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Detalles Bibliográficos
Autores principales:	Chupradit, Koollawat, Thongsin, Nontaphat, Tayapiwatana, Chatchai, Wattanapanitch, Methichit
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2022
Materias:	Lab Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262223/ https://www.ncbi.nlm.nih.gov/pubmed/35797389 http://dx.doi.org/10.1371/journal.pone.0270963

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