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Dual Antibody-Conjugated Amyloid Nanorods to Promote Selective Cell–Cell Interactions
[Image: see text] Grafting biomolecules on nanostructures’ surfaces is an increasingly used strategy to target pathogenic cells, with both diagnostic and therapeutic applications. However, nanomaterials monofunctionalized by conjugating a single type of ligand find limited uses in pathologies/therap...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262253/ https://www.ncbi.nlm.nih.gov/pubmed/33759489 http://dx.doi.org/10.1021/acsami.0c21996 |
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author | Wang, Weiqiang Gil-Garcia, Marcos Ventura, Salvador |
author_facet | Wang, Weiqiang Gil-Garcia, Marcos Ventura, Salvador |
author_sort | Wang, Weiqiang |
collection | PubMed |
description | [Image: see text] Grafting biomolecules on nanostructures’ surfaces is an increasingly used strategy to target pathogenic cells, with both diagnostic and therapeutic applications. However, nanomaterials monofunctionalized by conjugating a single type of ligand find limited uses in pathologies/therapies that require two or more targets/receptors to be targeted and/or activated with a single molecular entity simultaneously. Therefore, multivalent nanomaterials for dual- or multitargeting are attracting significant interest. This study provides a proof of concept of such nanostructures. We have recently developed a modular methodology that allows obtaining amyloid-based materials decorated with active globular domains. Here, this approach is exploited to generate functional amyloid fibrils displaying antibody capture moieties. A high antibody binding affinity and capacity for the resulting nanofibrils, whose size can be manipulated to obtain homogeneous nanorods with high biocompatibility, are demonstrated. These nanorods are then used for specific antibody-mediated targeting of different cell types. Simultaneous conjugation of these nanorods with different antibodies allows obtaining a mimic of a bispecific antibody that redirects T lymphocytes to tumoral cells, holding high potential for immunotherapy. Overall, the work illustrates a modular and straightforward strategy to obtain preparative quantities of multivalent antibody-functionalized nanomaterials with multitargeting properties without the need for covalent modification. |
format | Online Article Text |
id | pubmed-9262253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-92622532022-07-08 Dual Antibody-Conjugated Amyloid Nanorods to Promote Selective Cell–Cell Interactions Wang, Weiqiang Gil-Garcia, Marcos Ventura, Salvador ACS Appl Mater Interfaces [Image: see text] Grafting biomolecules on nanostructures’ surfaces is an increasingly used strategy to target pathogenic cells, with both diagnostic and therapeutic applications. However, nanomaterials monofunctionalized by conjugating a single type of ligand find limited uses in pathologies/therapies that require two or more targets/receptors to be targeted and/or activated with a single molecular entity simultaneously. Therefore, multivalent nanomaterials for dual- or multitargeting are attracting significant interest. This study provides a proof of concept of such nanostructures. We have recently developed a modular methodology that allows obtaining amyloid-based materials decorated with active globular domains. Here, this approach is exploited to generate functional amyloid fibrils displaying antibody capture moieties. A high antibody binding affinity and capacity for the resulting nanofibrils, whose size can be manipulated to obtain homogeneous nanorods with high biocompatibility, are demonstrated. These nanorods are then used for specific antibody-mediated targeting of different cell types. Simultaneous conjugation of these nanorods with different antibodies allows obtaining a mimic of a bispecific antibody that redirects T lymphocytes to tumoral cells, holding high potential for immunotherapy. Overall, the work illustrates a modular and straightforward strategy to obtain preparative quantities of multivalent antibody-functionalized nanomaterials with multitargeting properties without the need for covalent modification. American Chemical Society 2021-03-24 2021-04-07 /pmc/articles/PMC9262253/ /pubmed/33759489 http://dx.doi.org/10.1021/acsami.0c21996 Text en © 2021 American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Wang, Weiqiang Gil-Garcia, Marcos Ventura, Salvador Dual Antibody-Conjugated Amyloid Nanorods to Promote Selective Cell–Cell Interactions |
title | Dual
Antibody-Conjugated Amyloid Nanorods to Promote
Selective Cell–Cell Interactions |
title_full | Dual
Antibody-Conjugated Amyloid Nanorods to Promote
Selective Cell–Cell Interactions |
title_fullStr | Dual
Antibody-Conjugated Amyloid Nanorods to Promote
Selective Cell–Cell Interactions |
title_full_unstemmed | Dual
Antibody-Conjugated Amyloid Nanorods to Promote
Selective Cell–Cell Interactions |
title_short | Dual
Antibody-Conjugated Amyloid Nanorods to Promote
Selective Cell–Cell Interactions |
title_sort | dual
antibody-conjugated amyloid nanorods to promote
selective cell–cell interactions |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262253/ https://www.ncbi.nlm.nih.gov/pubmed/33759489 http://dx.doi.org/10.1021/acsami.0c21996 |
work_keys_str_mv | AT wangweiqiang dualantibodyconjugatedamyloidnanorodstopromoteselectivecellcellinteractions AT gilgarciamarcos dualantibodyconjugatedamyloidnanorodstopromoteselectivecellcellinteractions AT venturasalvador dualantibodyconjugatedamyloidnanorodstopromoteselectivecellcellinteractions |