Cargando…
Synthesis of the NarP response regulator of nitrate respiration in Escherichia coli is regulated at multiple levels by Hfq and small RNAs
Two-component systems (TCS) and small RNAs (sRNA) are widespread regulators that participate in the response and the adaptation of bacteria to their environments. TCSs and sRNAs mostly act at the transcriptional and post-transcriptional levels, respectively, and can be found integrated in regulatory...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262595/ https://www.ncbi.nlm.nih.gov/pubmed/35748881 http://dx.doi.org/10.1093/nar/gkac504 |
_version_ | 1784742534530990080 |
---|---|
author | Brosse, Anaïs Boudry, Pierre Walburger, Anne Magalon, Axel Guillier, Maude |
author_facet | Brosse, Anaïs Boudry, Pierre Walburger, Anne Magalon, Axel Guillier, Maude |
author_sort | Brosse, Anaïs |
collection | PubMed |
description | Two-component systems (TCS) and small RNAs (sRNA) are widespread regulators that participate in the response and the adaptation of bacteria to their environments. TCSs and sRNAs mostly act at the transcriptional and post-transcriptional levels, respectively, and can be found integrated in regulatory circuits, where TCSs control sRNAs transcription and/or sRNAs post-transcriptionally regulate TCSs synthesis. In response to nitrate and nitrite, the paralogous NarQ-NarP and NarX-NarL TCSs regulate the expression of genes involved in anaerobic respiration of these alternative electron acceptors to oxygen. In addition to the previously reported repression of NarP synthesis by the SdsN(137) sRNA, we show here that RprA, another Hfq-dependent sRNA, also negatively controls narP. Interestingly, the repression of narP by RprA actually relies on two independent mechanisms of control. The first is via the direct pairing of the central region of RprA to the narP translation initiation region and presumably occurs at the translation initiation level. In contrast, the second requires only the very 5′ end of the narP mRNA, which is targeted, most likely indirectly, by the full-length or the shorter, processed, form of RprA. In addition, our results raise the possibility of a direct role of Hfq in narP control, further illustrating the diversity of post-transcriptional regulation mechanisms in the synthesis of TCSs. |
format | Online Article Text |
id | pubmed-9262595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-92625952022-07-08 Synthesis of the NarP response regulator of nitrate respiration in Escherichia coli is regulated at multiple levels by Hfq and small RNAs Brosse, Anaïs Boudry, Pierre Walburger, Anne Magalon, Axel Guillier, Maude Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Two-component systems (TCS) and small RNAs (sRNA) are widespread regulators that participate in the response and the adaptation of bacteria to their environments. TCSs and sRNAs mostly act at the transcriptional and post-transcriptional levels, respectively, and can be found integrated in regulatory circuits, where TCSs control sRNAs transcription and/or sRNAs post-transcriptionally regulate TCSs synthesis. In response to nitrate and nitrite, the paralogous NarQ-NarP and NarX-NarL TCSs regulate the expression of genes involved in anaerobic respiration of these alternative electron acceptors to oxygen. In addition to the previously reported repression of NarP synthesis by the SdsN(137) sRNA, we show here that RprA, another Hfq-dependent sRNA, also negatively controls narP. Interestingly, the repression of narP by RprA actually relies on two independent mechanisms of control. The first is via the direct pairing of the central region of RprA to the narP translation initiation region and presumably occurs at the translation initiation level. In contrast, the second requires only the very 5′ end of the narP mRNA, which is targeted, most likely indirectly, by the full-length or the shorter, processed, form of RprA. In addition, our results raise the possibility of a direct role of Hfq in narP control, further illustrating the diversity of post-transcriptional regulation mechanisms in the synthesis of TCSs. Oxford University Press 2022-06-24 /pmc/articles/PMC9262595/ /pubmed/35748881 http://dx.doi.org/10.1093/nar/gkac504 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Gene regulation, Chromatin and Epigenetics Brosse, Anaïs Boudry, Pierre Walburger, Anne Magalon, Axel Guillier, Maude Synthesis of the NarP response regulator of nitrate respiration in Escherichia coli is regulated at multiple levels by Hfq and small RNAs |
title | Synthesis of the NarP response regulator of nitrate respiration in Escherichia coli is regulated at multiple levels by Hfq and small RNAs |
title_full | Synthesis of the NarP response regulator of nitrate respiration in Escherichia coli is regulated at multiple levels by Hfq and small RNAs |
title_fullStr | Synthesis of the NarP response regulator of nitrate respiration in Escherichia coli is regulated at multiple levels by Hfq and small RNAs |
title_full_unstemmed | Synthesis of the NarP response regulator of nitrate respiration in Escherichia coli is regulated at multiple levels by Hfq and small RNAs |
title_short | Synthesis of the NarP response regulator of nitrate respiration in Escherichia coli is regulated at multiple levels by Hfq and small RNAs |
title_sort | synthesis of the narp response regulator of nitrate respiration in escherichia coli is regulated at multiple levels by hfq and small rnas |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262595/ https://www.ncbi.nlm.nih.gov/pubmed/35748881 http://dx.doi.org/10.1093/nar/gkac504 |
work_keys_str_mv | AT brosseanais synthesisofthenarpresponseregulatorofnitraterespirationinescherichiacoliisregulatedatmultiplelevelsbyhfqandsmallrnas AT boudrypierre synthesisofthenarpresponseregulatorofnitraterespirationinescherichiacoliisregulatedatmultiplelevelsbyhfqandsmallrnas AT walburgeranne synthesisofthenarpresponseregulatorofnitraterespirationinescherichiacoliisregulatedatmultiplelevelsbyhfqandsmallrnas AT magalonaxel synthesisofthenarpresponseregulatorofnitraterespirationinescherichiacoliisregulatedatmultiplelevelsbyhfqandsmallrnas AT guilliermaude synthesisofthenarpresponseregulatorofnitraterespirationinescherichiacoliisregulatedatmultiplelevelsbyhfqandsmallrnas |