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An investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) silages

OBJECTIVE: This study aimed to investigate the fermentation profiles, bacterial community and predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) during ensiling. METHODS: First-cutting Sudangrass was harvested at the vegetative stage and ensiled in laboratory-scale silos (1...

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Autores principales: Wang, Siran, Li, Junfeng, Zhao, Jie, Dong, Zhihao, Shao, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Animal Bioscience 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262718/
https://www.ncbi.nlm.nih.gov/pubmed/34991212
http://dx.doi.org/10.5713/ab.21.0326
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author Wang, Siran
Li, Junfeng
Zhao, Jie
Dong, Zhihao
Shao, Tao
author_facet Wang, Siran
Li, Junfeng
Zhao, Jie
Dong, Zhihao
Shao, Tao
author_sort Wang, Siran
collection PubMed
description OBJECTIVE: This study aimed to investigate the fermentation profiles, bacterial community and predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) during ensiling. METHODS: First-cutting Sudangrass was harvested at the vegetative stage and ensiled in laboratory-scale silos (1 L capacity). Triplicate silos were sampled after 1, 3, 7, 15, 30, and 60 days of ensiling, respectively. The bacterial communities on day 3 and 60 were assessed through high-throughput sequencing technology, and 16S rRNA-gene predicted functional profiles were analyzed according to the Kyoto encyclopedia of genes and genomes using Tax4Fun. RESULTS: The Sudangrass silages showed good fermentation quality, indicated by higher lactic acid contents, and lower pH, butyric acid and ammonia nitrogen contents. The dominant genus Lactococcus on day 3 was replaced by Lactobacillus on day 60. The metabolism of amino acid, energy, cofactors and vitamins was restricted, and metabolism of nucleotide and carbohydrate was promoted after ensiling. The 1-phosphofructokinase and pyruvate kinase of bacterial community seemed to play important roles in stimulating the lactic acid fermentation, and the promotion of arginine deiminase could help lactic acid bacteria to tolerate the acidic environment. CONCLUSION: High-throughput sequencing technology combined with 16S rRNA gene-predicted functional analyses revealed the differences during the early and late stages of Sudangrass ensiling not only for distinct bacterial community but also for specific functional metabolites. The results could provide a comprehensive insight into bacterial community and metabolic characteristics to further improve the silage quality.
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spelling pubmed-92627182022-08-01 An investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) silages Wang, Siran Li, Junfeng Zhao, Jie Dong, Zhihao Shao, Tao Anim Biosci Article OBJECTIVE: This study aimed to investigate the fermentation profiles, bacterial community and predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) during ensiling. METHODS: First-cutting Sudangrass was harvested at the vegetative stage and ensiled in laboratory-scale silos (1 L capacity). Triplicate silos were sampled after 1, 3, 7, 15, 30, and 60 days of ensiling, respectively. The bacterial communities on day 3 and 60 were assessed through high-throughput sequencing technology, and 16S rRNA-gene predicted functional profiles were analyzed according to the Kyoto encyclopedia of genes and genomes using Tax4Fun. RESULTS: The Sudangrass silages showed good fermentation quality, indicated by higher lactic acid contents, and lower pH, butyric acid and ammonia nitrogen contents. The dominant genus Lactococcus on day 3 was replaced by Lactobacillus on day 60. The metabolism of amino acid, energy, cofactors and vitamins was restricted, and metabolism of nucleotide and carbohydrate was promoted after ensiling. The 1-phosphofructokinase and pyruvate kinase of bacterial community seemed to play important roles in stimulating the lactic acid fermentation, and the promotion of arginine deiminase could help lactic acid bacteria to tolerate the acidic environment. CONCLUSION: High-throughput sequencing technology combined with 16S rRNA gene-predicted functional analyses revealed the differences during the early and late stages of Sudangrass ensiling not only for distinct bacterial community but also for specific functional metabolites. The results could provide a comprehensive insight into bacterial community and metabolic characteristics to further improve the silage quality. Animal Bioscience 2022-08 2022-01-04 /pmc/articles/PMC9262718/ /pubmed/34991212 http://dx.doi.org/10.5713/ab.21.0326 Text en Copyright © 2022 by Animal Bioscience https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
spellingShingle Article
Wang, Siran
Li, Junfeng
Zhao, Jie
Dong, Zhihao
Shao, Tao
An investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) silages
title An investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) silages
title_full An investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) silages
title_fullStr An investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) silages
title_full_unstemmed An investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) silages
title_short An investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) silages
title_sort investigation on fermentative profile, microbial numbers, bacterial community diversity and their predicted metabolic characteristics of sudangrass (sorghum sudanense stapf.) silages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262718/
https://www.ncbi.nlm.nih.gov/pubmed/34991212
http://dx.doi.org/10.5713/ab.21.0326
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