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Comparative transcriptome analysis of synthetic and common wheat in response to salt stress
Salt stress reduces wheat yield. Therefore, improvement for enhanced salt stress tolerance is necessary for stable production. To understand the molecular mechanism of salt tolerance in common wheat and synthetic hexaploid (SH) wheat, RNA sequencing was performed on the roots of three wheat lines sa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262916/ https://www.ncbi.nlm.nih.gov/pubmed/35798819 http://dx.doi.org/10.1038/s41598-022-15733-2 |
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author | Nakayama, Rio Safi, Mohammad Taheb Ahmadzai, Waisuddin Sato, Kazuhiro Kawaura, Kanako |
author_facet | Nakayama, Rio Safi, Mohammad Taheb Ahmadzai, Waisuddin Sato, Kazuhiro Kawaura, Kanako |
author_sort | Nakayama, Rio |
collection | PubMed |
description | Salt stress reduces wheat yield. Therefore, improvement for enhanced salt stress tolerance is necessary for stable production. To understand the molecular mechanism of salt tolerance in common wheat and synthetic hexaploid (SH) wheat, RNA sequencing was performed on the roots of three wheat lines salt-tolerant SH wheat, salt-tolerant common wheat, and salt-sensitive common wheat. Differentially expressed genes (DEGs) in response to salt stress were characterized using gene ontology enrichment analysis. Salt tolerance in common wheat has been suggested to be mainly regulated by the activation of transporters. In contrast, salt tolerance in SH wheat is enhanced through up-regulation of the reactive oxygen species signaling pathway, other unknown pathways, and different ERF transcription factors. These results indicate that salt tolerance is differentially controlled between common wheat and SH wheat. Furthermore, QTL analysis was performed using the F(2) population derived from SH and salt-sensitive wheat. No statistically significant QTL was detected, suggesting that numerous QTLs with negligible contributions are involved in salt tolerance in SH wheat. We also identified DEGs specific to each line near one probable QTL. These findings show that SH wheat possesses salt tolerance mechanisms lacking in common wheat and may be potential breeding material for salt tolerance. |
format | Online Article Text |
id | pubmed-9262916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-92629162022-07-09 Comparative transcriptome analysis of synthetic and common wheat in response to salt stress Nakayama, Rio Safi, Mohammad Taheb Ahmadzai, Waisuddin Sato, Kazuhiro Kawaura, Kanako Sci Rep Article Salt stress reduces wheat yield. Therefore, improvement for enhanced salt stress tolerance is necessary for stable production. To understand the molecular mechanism of salt tolerance in common wheat and synthetic hexaploid (SH) wheat, RNA sequencing was performed on the roots of three wheat lines salt-tolerant SH wheat, salt-tolerant common wheat, and salt-sensitive common wheat. Differentially expressed genes (DEGs) in response to salt stress were characterized using gene ontology enrichment analysis. Salt tolerance in common wheat has been suggested to be mainly regulated by the activation of transporters. In contrast, salt tolerance in SH wheat is enhanced through up-regulation of the reactive oxygen species signaling pathway, other unknown pathways, and different ERF transcription factors. These results indicate that salt tolerance is differentially controlled between common wheat and SH wheat. Furthermore, QTL analysis was performed using the F(2) population derived from SH and salt-sensitive wheat. No statistically significant QTL was detected, suggesting that numerous QTLs with negligible contributions are involved in salt tolerance in SH wheat. We also identified DEGs specific to each line near one probable QTL. These findings show that SH wheat possesses salt tolerance mechanisms lacking in common wheat and may be potential breeding material for salt tolerance. Nature Publishing Group UK 2022-07-07 /pmc/articles/PMC9262916/ /pubmed/35798819 http://dx.doi.org/10.1038/s41598-022-15733-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nakayama, Rio Safi, Mohammad Taheb Ahmadzai, Waisuddin Sato, Kazuhiro Kawaura, Kanako Comparative transcriptome analysis of synthetic and common wheat in response to salt stress |
title | Comparative transcriptome analysis of synthetic and common wheat in response to salt stress |
title_full | Comparative transcriptome analysis of synthetic and common wheat in response to salt stress |
title_fullStr | Comparative transcriptome analysis of synthetic and common wheat in response to salt stress |
title_full_unstemmed | Comparative transcriptome analysis of synthetic and common wheat in response to salt stress |
title_short | Comparative transcriptome analysis of synthetic and common wheat in response to salt stress |
title_sort | comparative transcriptome analysis of synthetic and common wheat in response to salt stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262916/ https://www.ncbi.nlm.nih.gov/pubmed/35798819 http://dx.doi.org/10.1038/s41598-022-15733-2 |
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