Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants

Hairpin RNA (hpRNA) transgenes are the most successful RNA interference (RNAi) method in plants. Here, we show that hpRNA transgenes are invariably methylated in the inverted-repeat (IR) DNA and the adjacent promoter, causing transcriptional self-silencing. Nucleotide substitutions in the sense sequ...

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Detalles Bibliográficos
Autores principales: Zhang, Daai, Zhong, Chengcheng, Smith, Neil A., de Feyter, Robert, Greaves, Ian K., Swain, Steve M., Zhang, Ren, Wang, Ming-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263138/
https://www.ncbi.nlm.nih.gov/pubmed/35798725
http://dx.doi.org/10.1038/s41467-022-31641-5

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