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Effects of Tempeh Probiotics on Elderly With Cognitive Impairment

INTRODUCTION: Oral consumption of probiotics can alter Gut Microbiota by causing changes in the production of probiotic derivatives. Therefore, by utilizing Gut-Brain-Axis (GBA), probiotics could provide an opportunity for central nervous system (CNS) modulation, including cognitive function. Tempeh...

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Autores principales: Handajani, Yvonne Suzy, Turana, Yuda, Yogiara, Yogiara, Sugiyono, Sagita Pratiwi, Lamadong, Vincent, Widjaja, Nelly Tina, Christianto, Geovannie Audrey Moniqe, Suwanto, Antonius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263263/
https://www.ncbi.nlm.nih.gov/pubmed/35813939
http://dx.doi.org/10.3389/fnagi.2022.891773
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author Handajani, Yvonne Suzy
Turana, Yuda
Yogiara, Yogiara
Sugiyono, Sagita Pratiwi
Lamadong, Vincent
Widjaja, Nelly Tina
Christianto, Geovannie Audrey Moniqe
Suwanto, Antonius
author_facet Handajani, Yvonne Suzy
Turana, Yuda
Yogiara, Yogiara
Sugiyono, Sagita Pratiwi
Lamadong, Vincent
Widjaja, Nelly Tina
Christianto, Geovannie Audrey Moniqe
Suwanto, Antonius
author_sort Handajani, Yvonne Suzy
collection PubMed
description INTRODUCTION: Oral consumption of probiotics can alter Gut Microbiota by causing changes in the production of probiotic derivatives. Therefore, by utilizing Gut-Brain-Axis (GBA), probiotics could provide an opportunity for central nervous system (CNS) modulation, including cognitive function. Tempeh is a traditional Indonesian food rich in probiotics and beneficial for cognitive function. However, the type of probiotics that play a role in cognitive improvement and the number of probiotics needed for the benefits of increasing cognitive function was unknown. METHOD: This experimental study involved a total of 93 subjects, divided into 3 groups: A, B and C/control (n: 33, 32, and 28), who were provided with probiotic supplementation isolated from tempeh for 12 weeks intervention. Inclusion criteria were age > 60 years, and memory impairment with the third repetition value of Word List Memory Immediate Recall (WLMIR) < 7. Subjects with diabetes were excluded. Cognitive function examinations were carried out before and after treatment. The tempeh-derived probiotics were prepared trough several processes. Genomic isolation, detection of GABA-encoding genes, and species identification using the 16S-rRNA gene encoding were performed. RESULTS: The probiotics isolate used in the intervention was identified as Limosilactobacillus fermentum. We assigned this isolate as L. fermentum A2.8. The presence of the gene encoding GABA was found on this isolate. There was an increase in the cognitive domains of memory, learning process, and verbal fluency (p < 0.05) in group A (probiotics at concentration of 10(8) CFU/mL). Memory function, visuospatial, and verbal fluency improved (p < 0.05) in group B (probiotics at concentration of 10(7) CFU/mL). Only an increase in the memory domain was observed in the control group. Improvement of the learning process occurred only in group A (p = 0.006). CONCLUSION: Administration of probiotics derived from L. fermentum A2.8 increased the cognitive domains of memory, language and visuospatial function. However, probiotic supplementation at a concentration of 10(8) CFU/mL was better in improving the learning process. This study succeeded in detecting Lactic Acid Bacterial isolates L. fermentum A2.8 that enclosed gene encoding glutamate decarboxylase (gad) which is involved in the synthesis of -aminobutyric acid (GABA), a neurotransmitter vital for cognitive function.
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spelling pubmed-92632632022-07-09 Effects of Tempeh Probiotics on Elderly With Cognitive Impairment Handajani, Yvonne Suzy Turana, Yuda Yogiara, Yogiara Sugiyono, Sagita Pratiwi Lamadong, Vincent Widjaja, Nelly Tina Christianto, Geovannie Audrey Moniqe Suwanto, Antonius Front Aging Neurosci Aging Neuroscience INTRODUCTION: Oral consumption of probiotics can alter Gut Microbiota by causing changes in the production of probiotic derivatives. Therefore, by utilizing Gut-Brain-Axis (GBA), probiotics could provide an opportunity for central nervous system (CNS) modulation, including cognitive function. Tempeh is a traditional Indonesian food rich in probiotics and beneficial for cognitive function. However, the type of probiotics that play a role in cognitive improvement and the number of probiotics needed for the benefits of increasing cognitive function was unknown. METHOD: This experimental study involved a total of 93 subjects, divided into 3 groups: A, B and C/control (n: 33, 32, and 28), who were provided with probiotic supplementation isolated from tempeh for 12 weeks intervention. Inclusion criteria were age > 60 years, and memory impairment with the third repetition value of Word List Memory Immediate Recall (WLMIR) < 7. Subjects with diabetes were excluded. Cognitive function examinations were carried out before and after treatment. The tempeh-derived probiotics were prepared trough several processes. Genomic isolation, detection of GABA-encoding genes, and species identification using the 16S-rRNA gene encoding were performed. RESULTS: The probiotics isolate used in the intervention was identified as Limosilactobacillus fermentum. We assigned this isolate as L. fermentum A2.8. The presence of the gene encoding GABA was found on this isolate. There was an increase in the cognitive domains of memory, learning process, and verbal fluency (p < 0.05) in group A (probiotics at concentration of 10(8) CFU/mL). Memory function, visuospatial, and verbal fluency improved (p < 0.05) in group B (probiotics at concentration of 10(7) CFU/mL). Only an increase in the memory domain was observed in the control group. Improvement of the learning process occurred only in group A (p = 0.006). CONCLUSION: Administration of probiotics derived from L. fermentum A2.8 increased the cognitive domains of memory, language and visuospatial function. However, probiotic supplementation at a concentration of 10(8) CFU/mL was better in improving the learning process. This study succeeded in detecting Lactic Acid Bacterial isolates L. fermentum A2.8 that enclosed gene encoding glutamate decarboxylase (gad) which is involved in the synthesis of -aminobutyric acid (GABA), a neurotransmitter vital for cognitive function. Frontiers Media S.A. 2022-06-24 /pmc/articles/PMC9263263/ /pubmed/35813939 http://dx.doi.org/10.3389/fnagi.2022.891773 Text en Copyright © 2022 Handajani, Turana, Yogiara, Sugiyono, Lamadong, Widjaja, Christianto and Suwanto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Aging Neuroscience
Handajani, Yvonne Suzy
Turana, Yuda
Yogiara, Yogiara
Sugiyono, Sagita Pratiwi
Lamadong, Vincent
Widjaja, Nelly Tina
Christianto, Geovannie Audrey Moniqe
Suwanto, Antonius
Effects of Tempeh Probiotics on Elderly With Cognitive Impairment
title Effects of Tempeh Probiotics on Elderly With Cognitive Impairment
title_full Effects of Tempeh Probiotics on Elderly With Cognitive Impairment
title_fullStr Effects of Tempeh Probiotics on Elderly With Cognitive Impairment
title_full_unstemmed Effects of Tempeh Probiotics on Elderly With Cognitive Impairment
title_short Effects of Tempeh Probiotics on Elderly With Cognitive Impairment
title_sort effects of tempeh probiotics on elderly with cognitive impairment
topic Aging Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263263/
https://www.ncbi.nlm.nih.gov/pubmed/35813939
http://dx.doi.org/10.3389/fnagi.2022.891773
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