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Beneficial effect of capsaicin via TRPV4/EDH signals on mesenteric arterioles of normal and colitis mice

INTRODUCTION: Although capsaicin has long been used as food additive and medication worldwide, its actions on gastrointestinal tract as its most delivery pathway have not been well addressed. OBJECTIVES: In the present study, we aimed to study GI actions of capsaicin on mesenteric arterioles in norm...

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Autores principales: Zhang, Luyun, Lu, Wei, Lu, Cheng, Guo, Yanjun, Chen, Xiongying, Chen, Jun, Xu, Feng, Wan, Hanxing, Dong, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263647/
https://www.ncbi.nlm.nih.gov/pubmed/35777913
http://dx.doi.org/10.1016/j.jare.2021.11.001
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author Zhang, Luyun
Lu, Wei
Lu, Cheng
Guo, Yanjun
Chen, Xiongying
Chen, Jun
Xu, Feng
Wan, Hanxing
Dong, Hui
author_facet Zhang, Luyun
Lu, Wei
Lu, Cheng
Guo, Yanjun
Chen, Xiongying
Chen, Jun
Xu, Feng
Wan, Hanxing
Dong, Hui
author_sort Zhang, Luyun
collection PubMed
description INTRODUCTION: Although capsaicin has long been used as food additive and medication worldwide, its actions on gastrointestinal tract as its most delivery pathway have not been well addressed. OBJECTIVES: In the present study, we aimed to study GI actions of capsaicin on mesenteric arterioles in normal and colitis mice and to elucidate the underlying mechanisms. METHODS: Vasorelaxation of human submucosal arterioles and the mesenteric arterioles from wide-type (WT) mice, TRPV1(−/−) and TRPV4(−/−) (KO) mice were measured. The expression and function of TRPV channels in endothelial cells were examined by q-PCR, immunostaining, Ca(2+) imaging and membrane potential measurements. RESULTS: Capsaicin dose-dependently induced vasorelaxation of human submucosal arterioles and mouse mesenteric arterioles in vitro and in vivo through endothelium-dependent hyperpolarization (EDH), nitric oxide (NO), and prostacyclin (PGI(2)). Using TRPV1 and TRPV4 KO mice, we found that capsaicin-induced vasorelaxation was predominately through TRPV4/EDH, but marginally through TRPV1/NO/PGI(2). Capsaicin induced hyperpolarization through activation of endothelial TRPV4 channels and intermediate-conductance of Ca(2+)-activated K(+) channels to finally stimulate vasorelaxation. Importantly, capsaicin exerted anti-colitis action by rescuing the impaired ACh-induced vasorelaxation in WT colitis mice but not in TRPV4 KO colitis mice. CONCLUSIONS: Capsaicin increases intestinal mucosal blood perfusion to potentially prevent/treat colitis through a novel TRPV4/EDH-dependent vasorelaxation of submucosal arterioles in health and colitis. This study further supports our previous notion that TRPV4/EDH in mesenteric circulation plays a critical role in the pathogenesis of colitis.
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spelling pubmed-92636472022-07-09 Beneficial effect of capsaicin via TRPV4/EDH signals on mesenteric arterioles of normal and colitis mice Zhang, Luyun Lu, Wei Lu, Cheng Guo, Yanjun Chen, Xiongying Chen, Jun Xu, Feng Wan, Hanxing Dong, Hui J Adv Res Original Article INTRODUCTION: Although capsaicin has long been used as food additive and medication worldwide, its actions on gastrointestinal tract as its most delivery pathway have not been well addressed. OBJECTIVES: In the present study, we aimed to study GI actions of capsaicin on mesenteric arterioles in normal and colitis mice and to elucidate the underlying mechanisms. METHODS: Vasorelaxation of human submucosal arterioles and the mesenteric arterioles from wide-type (WT) mice, TRPV1(−/−) and TRPV4(−/−) (KO) mice were measured. The expression and function of TRPV channels in endothelial cells were examined by q-PCR, immunostaining, Ca(2+) imaging and membrane potential measurements. RESULTS: Capsaicin dose-dependently induced vasorelaxation of human submucosal arterioles and mouse mesenteric arterioles in vitro and in vivo through endothelium-dependent hyperpolarization (EDH), nitric oxide (NO), and prostacyclin (PGI(2)). Using TRPV1 and TRPV4 KO mice, we found that capsaicin-induced vasorelaxation was predominately through TRPV4/EDH, but marginally through TRPV1/NO/PGI(2). Capsaicin induced hyperpolarization through activation of endothelial TRPV4 channels and intermediate-conductance of Ca(2+)-activated K(+) channels to finally stimulate vasorelaxation. Importantly, capsaicin exerted anti-colitis action by rescuing the impaired ACh-induced vasorelaxation in WT colitis mice but not in TRPV4 KO colitis mice. CONCLUSIONS: Capsaicin increases intestinal mucosal blood perfusion to potentially prevent/treat colitis through a novel TRPV4/EDH-dependent vasorelaxation of submucosal arterioles in health and colitis. This study further supports our previous notion that TRPV4/EDH in mesenteric circulation plays a critical role in the pathogenesis of colitis. Elsevier 2021-11-07 /pmc/articles/PMC9263647/ /pubmed/35777913 http://dx.doi.org/10.1016/j.jare.2021.11.001 Text en © 2022 The Authors. Published by Elsevier B.V. on behalf of Cairo University. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Zhang, Luyun
Lu, Wei
Lu, Cheng
Guo, Yanjun
Chen, Xiongying
Chen, Jun
Xu, Feng
Wan, Hanxing
Dong, Hui
Beneficial effect of capsaicin via TRPV4/EDH signals on mesenteric arterioles of normal and colitis mice
title Beneficial effect of capsaicin via TRPV4/EDH signals on mesenteric arterioles of normal and colitis mice
title_full Beneficial effect of capsaicin via TRPV4/EDH signals on mesenteric arterioles of normal and colitis mice
title_fullStr Beneficial effect of capsaicin via TRPV4/EDH signals on mesenteric arterioles of normal and colitis mice
title_full_unstemmed Beneficial effect of capsaicin via TRPV4/EDH signals on mesenteric arterioles of normal and colitis mice
title_short Beneficial effect of capsaicin via TRPV4/EDH signals on mesenteric arterioles of normal and colitis mice
title_sort beneficial effect of capsaicin via trpv4/edh signals on mesenteric arterioles of normal and colitis mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263647/
https://www.ncbi.nlm.nih.gov/pubmed/35777913
http://dx.doi.org/10.1016/j.jare.2021.11.001
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