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Accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold

Ideal artificial tissue scaffolds should provide an in vitro microenvironment comparable to native human skin tissue to direct cell functions, regulate tissue homeostasis, and promote tissue regeneration. A sandwich-like composite scaffold consisting of a hydrogel layer and two aligned nanofibre lay...

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Detalles Bibliográficos
Autores principales: Mohd Razali, Nur Adila, Lin, Wei-Chih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263994/
https://www.ncbi.nlm.nih.gov/pubmed/35813981
http://dx.doi.org/10.1016/j.mtbio.2022.100347
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author Mohd Razali, Nur Adila
Lin, Wei-Chih
author_facet Mohd Razali, Nur Adila
Lin, Wei-Chih
author_sort Mohd Razali, Nur Adila
collection PubMed
description Ideal artificial tissue scaffolds should provide an in vitro microenvironment comparable to native human skin tissue to direct cell functions, regulate tissue homeostasis, and promote tissue regeneration. A sandwich-like composite scaffold consisting of a hydrogel layer and two aligned nanofibre layers was fabricated and applied as a wound-healing dressing. Gentamicin was preloaded into the hydrogel middle layer and naturally released for antibacterial activity during the healing period. Nanofibrous layers embedded on the top and bottom surfaces of the hydrogel improved the tensile strength fivefold (1560 ​kPa and 465% strain) while serving as a diffusion barrier to reduce the gentamicin initial burst release (30%–15%). Inspired by the extracellular matrix (ECM), the surface of nanofibre top layer was patterned with triangular microarrays using micro-moulding approach to reflect the multidimensional structure of ECM. Biocompatibility of the scaffold is proven from cytotoxicity and haemolysis studies. Fibroblast cells revealed a highly elongated and consistent alignment modulated by the micropatterned fibrous layer and directed their migration towards the wound area. Excisional wounds treated with the scaffold promoted 97.49% wound closure with low inflammation and rapid re-epithelialization and angiogenesis. This scaffold, with its tailored functionality capable of accelerating wound healing, has high potential in tissue engineering applications.
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spelling pubmed-92639942022-07-09 Accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold Mohd Razali, Nur Adila Lin, Wei-Chih Mater Today Bio Full Length Article Ideal artificial tissue scaffolds should provide an in vitro microenvironment comparable to native human skin tissue to direct cell functions, regulate tissue homeostasis, and promote tissue regeneration. A sandwich-like composite scaffold consisting of a hydrogel layer and two aligned nanofibre layers was fabricated and applied as a wound-healing dressing. Gentamicin was preloaded into the hydrogel middle layer and naturally released for antibacterial activity during the healing period. Nanofibrous layers embedded on the top and bottom surfaces of the hydrogel improved the tensile strength fivefold (1560 ​kPa and 465% strain) while serving as a diffusion barrier to reduce the gentamicin initial burst release (30%–15%). Inspired by the extracellular matrix (ECM), the surface of nanofibre top layer was patterned with triangular microarrays using micro-moulding approach to reflect the multidimensional structure of ECM. Biocompatibility of the scaffold is proven from cytotoxicity and haemolysis studies. Fibroblast cells revealed a highly elongated and consistent alignment modulated by the micropatterned fibrous layer and directed their migration towards the wound area. Excisional wounds treated with the scaffold promoted 97.49% wound closure with low inflammation and rapid re-epithelialization and angiogenesis. This scaffold, with its tailored functionality capable of accelerating wound healing, has high potential in tissue engineering applications. Elsevier 2022-06-30 /pmc/articles/PMC9263994/ /pubmed/35813981 http://dx.doi.org/10.1016/j.mtbio.2022.100347 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Mohd Razali, Nur Adila
Lin, Wei-Chih
Accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold
title Accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold
title_full Accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold
title_fullStr Accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold
title_full_unstemmed Accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold
title_short Accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold
title_sort accelerating the excisional wound closure by using the patterned microstructural nanofibrous mats/gentamicin-loaded hydrogel composite scaffold
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263994/
https://www.ncbi.nlm.nih.gov/pubmed/35813981
http://dx.doi.org/10.1016/j.mtbio.2022.100347
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