Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides

[Image: see text] Collisionally activated dissociation (CAD), infrared multiphoton dissociation (IRMPD), ultraviolet photodissociation (UVPD), electron capture dissociation and electron detachment dissociation (EDD) experiments were conducted on a set of phosphopeptides, in a Fourier transform ion c...

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Autores principales: Paris, Johanna, Theisen, Alina, Marzullo, Bryan P., Haris, Anisha, Morgan, Tomos E., Barrow, Mark P., O’Hara, John, O’Connor, Peter B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264387/
https://www.ncbi.nlm.nih.gov/pubmed/35604791
http://dx.doi.org/10.1021/jasms.1c00353
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author Paris, Johanna
Theisen, Alina
Marzullo, Bryan P.
Haris, Anisha
Morgan, Tomos E.
Barrow, Mark P.
O’Hara, John
O’Connor, Peter B.
author_facet Paris, Johanna
Theisen, Alina
Marzullo, Bryan P.
Haris, Anisha
Morgan, Tomos E.
Barrow, Mark P.
O’Hara, John
O’Connor, Peter B.
author_sort Paris, Johanna
collection PubMed
description [Image: see text] Collisionally activated dissociation (CAD), infrared multiphoton dissociation (IRMPD), ultraviolet photodissociation (UVPD), electron capture dissociation and electron detachment dissociation (EDD) experiments were conducted on a set of phosphopeptides, in a Fourier transform ion cyclotron resonance mass spectrometer. The fragmentation patterns were compared and varied according to the fragmentation mechanisms and the composition of the peptides. CAD and IRMPD produced similar fragmentation profiles of the phosphopeptides, while UVPD produced a large number of complementary fragments. Electron-based dissociation techniques displayed lower fragmentation efficiencies, despite retaining the labile phosphate group, and drastically different fragmentation profiles. EDD produced complex spectra whose interpretation proved challenging.
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spelling pubmed-92643872022-07-09 Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides Paris, Johanna Theisen, Alina Marzullo, Bryan P. Haris, Anisha Morgan, Tomos E. Barrow, Mark P. O’Hara, John O’Connor, Peter B. J Am Soc Mass Spectrom [Image: see text] Collisionally activated dissociation (CAD), infrared multiphoton dissociation (IRMPD), ultraviolet photodissociation (UVPD), electron capture dissociation and electron detachment dissociation (EDD) experiments were conducted on a set of phosphopeptides, in a Fourier transform ion cyclotron resonance mass spectrometer. The fragmentation patterns were compared and varied according to the fragmentation mechanisms and the composition of the peptides. CAD and IRMPD produced similar fragmentation profiles of the phosphopeptides, while UVPD produced a large number of complementary fragments. Electron-based dissociation techniques displayed lower fragmentation efficiencies, despite retaining the labile phosphate group, and drastically different fragmentation profiles. EDD produced complex spectra whose interpretation proved challenging. American Chemical Society 2022-05-23 2022-07-06 /pmc/articles/PMC9264387/ /pubmed/35604791 http://dx.doi.org/10.1021/jasms.1c00353 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Paris, Johanna
Theisen, Alina
Marzullo, Bryan P.
Haris, Anisha
Morgan, Tomos E.
Barrow, Mark P.
O’Hara, John
O’Connor, Peter B.
Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides
title Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides
title_full Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides
title_fullStr Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides
title_full_unstemmed Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides
title_short Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides
title_sort multimodal tandem mass spectrometry techniques for the analysis of phosphopeptides
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264387/
https://www.ncbi.nlm.nih.gov/pubmed/35604791
http://dx.doi.org/10.1021/jasms.1c00353
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