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Composition of Nonextractable Polyphenols from Sweet Cherry Pomace Determined by DART-Orbitrap-HRMS and Their In Vitro and In Vivo Potential Antioxidant, Antiaging, and Neuroprotective Activities
[Image: see text] Sweet cherry pomace is an important source of phenolic compounds with beneficial health properties. As after the extraction of phenolic compounds, a phenolic fraction called nonextractable polyphenols (NEPs) remains usually retained in the extraction residue, alkaline and acid hydr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264388/ https://www.ncbi.nlm.nih.gov/pubmed/35729789 http://dx.doi.org/10.1021/acs.jafc.2c03346 |
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author | Domínguez-Rodríguez, Gloria Ramón Vidal, Daniel Martorell, Patricia Plaza, Merichel Marina, María Luisa |
author_facet | Domínguez-Rodríguez, Gloria Ramón Vidal, Daniel Martorell, Patricia Plaza, Merichel Marina, María Luisa |
author_sort | Domínguez-Rodríguez, Gloria |
collection | PubMed |
description | [Image: see text] Sweet cherry pomace is an important source of phenolic compounds with beneficial health properties. As after the extraction of phenolic compounds, a phenolic fraction called nonextractable polyphenols (NEPs) remains usually retained in the extraction residue, alkaline and acid hydrolyses and enzymatic-assisted extraction (EAE) were carried out in this work to recover NEPs from the residue of conventional extraction from sweet cherry pomace. In vitro and in vivo evaluation of the antioxidant, antihypertensive, antiaging, and neuroprotective capacities employing Caenorhabditis elegans was achieved for the first time. Extractable phenolic compounds and NEPs were separated and identified by families by high-performance thin-layer chromatography (HPTLC) with UV/Vis detection. A total of 39 phenolic compounds were tentatively identified in all extracts by direct analysis in real-time high-resolution mass spectrometry (DART-Orbitrap-HRMS). EAE extracts presented the highest in vitro and in vivo antioxidant capacity as well as the highest in vivo antiaging and neuroprotective capacities. These results showed that NEPs with interesting biological properties are retained in the extraction residue, being usually underestimated and discarded. |
format | Online Article Text |
id | pubmed-9264388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-92643882022-07-09 Composition of Nonextractable Polyphenols from Sweet Cherry Pomace Determined by DART-Orbitrap-HRMS and Their In Vitro and In Vivo Potential Antioxidant, Antiaging, and Neuroprotective Activities Domínguez-Rodríguez, Gloria Ramón Vidal, Daniel Martorell, Patricia Plaza, Merichel Marina, María Luisa J Agric Food Chem [Image: see text] Sweet cherry pomace is an important source of phenolic compounds with beneficial health properties. As after the extraction of phenolic compounds, a phenolic fraction called nonextractable polyphenols (NEPs) remains usually retained in the extraction residue, alkaline and acid hydrolyses and enzymatic-assisted extraction (EAE) were carried out in this work to recover NEPs from the residue of conventional extraction from sweet cherry pomace. In vitro and in vivo evaluation of the antioxidant, antihypertensive, antiaging, and neuroprotective capacities employing Caenorhabditis elegans was achieved for the first time. Extractable phenolic compounds and NEPs were separated and identified by families by high-performance thin-layer chromatography (HPTLC) with UV/Vis detection. A total of 39 phenolic compounds were tentatively identified in all extracts by direct analysis in real-time high-resolution mass spectrometry (DART-Orbitrap-HRMS). EAE extracts presented the highest in vitro and in vivo antioxidant capacity as well as the highest in vivo antiaging and neuroprotective capacities. These results showed that NEPs with interesting biological properties are retained in the extraction residue, being usually underestimated and discarded. American Chemical Society 2022-06-22 2022-07-06 /pmc/articles/PMC9264388/ /pubmed/35729789 http://dx.doi.org/10.1021/acs.jafc.2c03346 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Domínguez-Rodríguez, Gloria Ramón Vidal, Daniel Martorell, Patricia Plaza, Merichel Marina, María Luisa Composition of Nonextractable Polyphenols from Sweet Cherry Pomace Determined by DART-Orbitrap-HRMS and Their In Vitro and In Vivo Potential Antioxidant, Antiaging, and Neuroprotective Activities |
title | Composition of Nonextractable Polyphenols from Sweet
Cherry Pomace Determined by DART-Orbitrap-HRMS and Their In
Vitro and In Vivo Potential Antioxidant,
Antiaging, and Neuroprotective Activities |
title_full | Composition of Nonextractable Polyphenols from Sweet
Cherry Pomace Determined by DART-Orbitrap-HRMS and Their In
Vitro and In Vivo Potential Antioxidant,
Antiaging, and Neuroprotective Activities |
title_fullStr | Composition of Nonextractable Polyphenols from Sweet
Cherry Pomace Determined by DART-Orbitrap-HRMS and Their In
Vitro and In Vivo Potential Antioxidant,
Antiaging, and Neuroprotective Activities |
title_full_unstemmed | Composition of Nonextractable Polyphenols from Sweet
Cherry Pomace Determined by DART-Orbitrap-HRMS and Their In
Vitro and In Vivo Potential Antioxidant,
Antiaging, and Neuroprotective Activities |
title_short | Composition of Nonextractable Polyphenols from Sweet
Cherry Pomace Determined by DART-Orbitrap-HRMS and Their In
Vitro and In Vivo Potential Antioxidant,
Antiaging, and Neuroprotective Activities |
title_sort | composition of nonextractable polyphenols from sweet
cherry pomace determined by dart-orbitrap-hrms and their in
vitro and in vivo potential antioxidant,
antiaging, and neuroprotective activities |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264388/ https://www.ncbi.nlm.nih.gov/pubmed/35729789 http://dx.doi.org/10.1021/acs.jafc.2c03346 |
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