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Physiological and Molecular Aspects of Two Thymus Species Differently Sensitive to Drought Stress
Drought is one of the most important threats to plants and agriculture. Here, the effects of four drought levels (90%, 55%, 40%, and 25% field capacity) on the relative water content (RWC), chlorophyll and carotenoids levels, and mRNA gene expression of metabolic enzymes in Thymus vulgaris (as sensi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264393/ https://www.ncbi.nlm.nih.gov/pubmed/35822781 http://dx.doi.org/10.3390/biotech11020008 |
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author | Ashrafi, Mohsen Azimi-Moqadam, Mohammad-Reza MohseniFard, Ehsan Shekari, Farid Jafary, Hossein Moradi, Parviz Pucci, Mariachiara Abate, Giulia Mastinu, Andrea |
author_facet | Ashrafi, Mohsen Azimi-Moqadam, Mohammad-Reza MohseniFard, Ehsan Shekari, Farid Jafary, Hossein Moradi, Parviz Pucci, Mariachiara Abate, Giulia Mastinu, Andrea |
author_sort | Ashrafi, Mohsen |
collection | PubMed |
description | Drought is one of the most important threats to plants and agriculture. Here, the effects of four drought levels (90%, 55%, 40%, and 25% field capacity) on the relative water content (RWC), chlorophyll and carotenoids levels, and mRNA gene expression of metabolic enzymes in Thymus vulgaris (as sensitive to drought) and Thymus kotschyanus (as a drought-tolerant species) were evaluated. The physiological results showed that the treatment predominantly affected the RWC, chlorophyll, and carotenoids content. The gene expression analysis demonstrated that moderate and severe drought stress had greater effects on the expression of histone deacetylase-6 (HDA-6) and acetyl-CoA synthetase in both Thymus species. Pyruvate decarboxylase-1 (PDC-1) was upregulated in Thymus vulgaris at high drought levels. Finally, succinyl CoA ligase was not affected by drought stress in either species. Data confirmed water stress is able to alter the gene expression of specific enzymes. Furthermore, our results suggest that PDC-1 expression is independent from HDA-6 and the increased expression of ACS can be due to the activation of new pathways involved in carbohydrate production. |
format | Online Article Text |
id | pubmed-9264393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92643932022-07-09 Physiological and Molecular Aspects of Two Thymus Species Differently Sensitive to Drought Stress Ashrafi, Mohsen Azimi-Moqadam, Mohammad-Reza MohseniFard, Ehsan Shekari, Farid Jafary, Hossein Moradi, Parviz Pucci, Mariachiara Abate, Giulia Mastinu, Andrea BioTech (Basel) Article Drought is one of the most important threats to plants and agriculture. Here, the effects of four drought levels (90%, 55%, 40%, and 25% field capacity) on the relative water content (RWC), chlorophyll and carotenoids levels, and mRNA gene expression of metabolic enzymes in Thymus vulgaris (as sensitive to drought) and Thymus kotschyanus (as a drought-tolerant species) were evaluated. The physiological results showed that the treatment predominantly affected the RWC, chlorophyll, and carotenoids content. The gene expression analysis demonstrated that moderate and severe drought stress had greater effects on the expression of histone deacetylase-6 (HDA-6) and acetyl-CoA synthetase in both Thymus species. Pyruvate decarboxylase-1 (PDC-1) was upregulated in Thymus vulgaris at high drought levels. Finally, succinyl CoA ligase was not affected by drought stress in either species. Data confirmed water stress is able to alter the gene expression of specific enzymes. Furthermore, our results suggest that PDC-1 expression is independent from HDA-6 and the increased expression of ACS can be due to the activation of new pathways involved in carbohydrate production. MDPI 2022-03-23 /pmc/articles/PMC9264393/ /pubmed/35822781 http://dx.doi.org/10.3390/biotech11020008 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ashrafi, Mohsen Azimi-Moqadam, Mohammad-Reza MohseniFard, Ehsan Shekari, Farid Jafary, Hossein Moradi, Parviz Pucci, Mariachiara Abate, Giulia Mastinu, Andrea Physiological and Molecular Aspects of Two Thymus Species Differently Sensitive to Drought Stress |
title | Physiological and Molecular Aspects of Two Thymus Species Differently Sensitive to Drought Stress |
title_full | Physiological and Molecular Aspects of Two Thymus Species Differently Sensitive to Drought Stress |
title_fullStr | Physiological and Molecular Aspects of Two Thymus Species Differently Sensitive to Drought Stress |
title_full_unstemmed | Physiological and Molecular Aspects of Two Thymus Species Differently Sensitive to Drought Stress |
title_short | Physiological and Molecular Aspects of Two Thymus Species Differently Sensitive to Drought Stress |
title_sort | physiological and molecular aspects of two thymus species differently sensitive to drought stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264393/ https://www.ncbi.nlm.nih.gov/pubmed/35822781 http://dx.doi.org/10.3390/biotech11020008 |
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