Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation

The Shadoo and PrP prion protein family members are thought to be functionally related, but previous knockdown/knockout experiments in early mouse embryogenesis have provided seemingly contradictory results. In particular, Shadoo was found to be indispensable in the absence of PrP in knockdown analy...

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Detalles Bibliográficos
Autores principales: Rau, Andrea, Passet, Bruno, Castille, Johan, Daniel-Carlier, Nathalie, Asset, Alexandre, Lecardonnel, Jérome, Moroldo, Marco, Jaffrézic, Florence, Laloë, Denis, Moazami-Goudarzi, Katayoun, Vilotte, Jean-Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264527/
https://www.ncbi.nlm.nih.gov/pubmed/35799279
http://dx.doi.org/10.1186/s13567-022-01075-4
Descripción
Sumario:The Shadoo and PrP prion protein family members are thought to be functionally related, but previous knockdown/knockout experiments in early mouse embryogenesis have provided seemingly contradictory results. In particular, Shadoo was found to be indispensable in the absence of PrP in knockdown analyses, but a double-knockout of the two had little phenotypic impact. We investigated this apparent discrepancy by comparing transcriptomes of WT, Prnp(0/0) and Prnp(0/0)Sprn(0/0) E6.5 mouse embryos following inoculation by Sprn- or Prnp-ShRNA lentiviral vectors. Our results suggest the possibility of genetic adaptation in Prnp(0/0)Sprn(0/0) mice, thus providing a potential explanation for their previously observed resilience.

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