Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation

The Shadoo and PrP prion protein family members are thought to be functionally related, but previous knockdown/knockout experiments in early mouse embryogenesis have provided seemingly contradictory results. In particular, Shadoo was found to be indispensable in the absence of PrP in knockdown analy...

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Autores principales: Rau, Andrea, Passet, Bruno, Castille, Johan, Daniel-Carlier, Nathalie, Asset, Alexandre, Lecardonnel, Jérome, Moroldo, Marco, Jaffrézic, Florence, Laloë, Denis, Moazami-Goudarzi, Katayoun, Vilotte, Jean-Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264527/
https://www.ncbi.nlm.nih.gov/pubmed/35799279
http://dx.doi.org/10.1186/s13567-022-01075-4
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author Rau, Andrea
Passet, Bruno
Castille, Johan
Daniel-Carlier, Nathalie
Asset, Alexandre
Lecardonnel, Jérome
Moroldo, Marco
Jaffrézic, Florence
Laloë, Denis
Moazami-Goudarzi, Katayoun
Vilotte, Jean-Luc
author_facet Rau, Andrea
Passet, Bruno
Castille, Johan
Daniel-Carlier, Nathalie
Asset, Alexandre
Lecardonnel, Jérome
Moroldo, Marco
Jaffrézic, Florence
Laloë, Denis
Moazami-Goudarzi, Katayoun
Vilotte, Jean-Luc
author_sort Rau, Andrea
collection PubMed
description The Shadoo and PrP prion protein family members are thought to be functionally related, but previous knockdown/knockout experiments in early mouse embryogenesis have provided seemingly contradictory results. In particular, Shadoo was found to be indispensable in the absence of PrP in knockdown analyses, but a double-knockout of the two had little phenotypic impact. We investigated this apparent discrepancy by comparing transcriptomes of WT, Prnp(0/0) and Prnp(0/0)Sprn(0/0) E6.5 mouse embryos following inoculation by Sprn- or Prnp-ShRNA lentiviral vectors. Our results suggest the possibility of genetic adaptation in Prnp(0/0)Sprn(0/0) mice, thus providing a potential explanation for their previously observed resilience.
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spelling pubmed-92645272022-07-09 Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation Rau, Andrea Passet, Bruno Castille, Johan Daniel-Carlier, Nathalie Asset, Alexandre Lecardonnel, Jérome Moroldo, Marco Jaffrézic, Florence Laloë, Denis Moazami-Goudarzi, Katayoun Vilotte, Jean-Luc Vet Res Short Report The Shadoo and PrP prion protein family members are thought to be functionally related, but previous knockdown/knockout experiments in early mouse embryogenesis have provided seemingly contradictory results. In particular, Shadoo was found to be indispensable in the absence of PrP in knockdown analyses, but a double-knockout of the two had little phenotypic impact. We investigated this apparent discrepancy by comparing transcriptomes of WT, Prnp(0/0) and Prnp(0/0)Sprn(0/0) E6.5 mouse embryos following inoculation by Sprn- or Prnp-ShRNA lentiviral vectors. Our results suggest the possibility of genetic adaptation in Prnp(0/0)Sprn(0/0) mice, thus providing a potential explanation for their previously observed resilience. BioMed Central 2022-07-07 2022 /pmc/articles/PMC9264527/ /pubmed/35799279 http://dx.doi.org/10.1186/s13567-022-01075-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Rau, Andrea
Passet, Bruno
Castille, Johan
Daniel-Carlier, Nathalie
Asset, Alexandre
Lecardonnel, Jérome
Moroldo, Marco
Jaffrézic, Florence
Laloë, Denis
Moazami-Goudarzi, Katayoun
Vilotte, Jean-Luc
Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation
title Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation
title_full Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation
title_fullStr Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation
title_full_unstemmed Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation
title_short Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation
title_sort potential genetic robustness of prnp and sprn double knockout mouse embryos towards shrna-lentiviral inoculation
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264527/
https://www.ncbi.nlm.nih.gov/pubmed/35799279
http://dx.doi.org/10.1186/s13567-022-01075-4
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