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Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis

BACKGROUND: The true diploid frog, Xenopus tropicalis (X. tropicalis) is an excellent genetic model organism. To date, the CRISPR/Cas-mediated genome editing methods established in this species are mostly based on SpCas9 that requires the stringent NGG protospacer-adjacent motif (PAM) for target rec...

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Detalles Bibliográficos
Autores principales: Shi, Zhaoying, Jiang, Hao, Liu, Guanghui, Shi, Songyuan, Zhang, Xuan, Chen, Yonglong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264700/
https://www.ncbi.nlm.nih.gov/pubmed/35804405
http://dx.doi.org/10.1186/s13578-022-00841-3