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Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis

BACKGROUND: The true diploid frog, Xenopus tropicalis (X. tropicalis) is an excellent genetic model organism. To date, the CRISPR/Cas-mediated genome editing methods established in this species are mostly based on SpCas9 that requires the stringent NGG protospacer-adjacent motif (PAM) for target rec...

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Detalles Bibliográficos
Autores principales:	Shi, Zhaoying, Jiang, Hao, Liu, Guanghui, Shi, Songyuan, Zhang, Xuan, Chen, Yonglong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264700/ https://www.ncbi.nlm.nih.gov/pubmed/35804405 http://dx.doi.org/10.1186/s13578-022-00841-3

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