Differential Responses of Digesta- and Mucosa-Associated Jejunal Microbiota of Hu Sheep to Pelleted and Non-Pelleted High-Grain Diets

SIMPLE SUMMARY: Currently, feeding ruminants high-grain (HG) diets is a common feeding pattern, but this practice can have detrimental effects on these animals in the form of altering their gut microbiota. The aim of this study was to explore the structural and functional responses of jejunal digesta- and mucosa-associated microbiota to the low-grain as well as non-pelleted and pelleted high-grain (HP) diets. The results indicated that different diets altered the composition of digesta- and mucosa-associated microbiota, and the abundance of acid-producing bacteria was increased in both jejunal digesta and mucosa after the HG and HP diets. Meanwhile, the HP diets somewhat changed the impact of the HG diets, as HP diets reduced the proliferation of pro-inflammatory bacteria in the jejunal digesta and mucosa when compared to HG diets. ABSTRACT: In the present study, we utilized 16S rRNA sequencing to uncover the impacts of non-pelleted (HG) or high-grain pelleted (HP) diets on the microbial structure and potential functions of digesta- and mucosa-associated microbiota in the jejunum of Hu sheep. Here, we randomly assigned 15 healthy male Hu sheep into three groups and fed the control diets (CON), HG, and HP diets, respectively. The experiment period was 60 days. The HP diets had the same nutritional ingredients as the HG diets but in pelleted form. At the finish of the experiment, the jejunal digesta and mucosa were gathered for microbial sequencing. The results of PCoA and PERMANOVA showed that different dietary treatments had significant impact (p < 0.05) on digesta- and mucosa-associated microbiota in the jejunum of Hu sheep. For specific differences, HG diets significantly increased (p < 0.05) the abundance of some acid-producing bacteria in both jejunal digesta (Bifidobacterium, OTU151, and OTU16) and mucosa (Rikenellaceae RC9 gut group, and Bifidobacterium) of Hu sheep compared with the CON diets. Besides the similar effects of the HG diets (increased the acid-producing bacteria such as Olsenella, Pseudoramibacter, and Shuttleworthia), our results also showed that the HP diets significantly decreased (p < 0.05) the abundance of some pro-inflammatory bacteria in the jejunal digesta (Mogibacterium, and Marvinbryantia) and mucosa (Chitinophaga, and Candidatus Saccharimonas) of Hu sheep compared with the HG diets. Collectively, these findings contributed to enriching the knowledge about the effects of HG diets on the structure and function of intestinal microbiota in ruminants.