Infectious Disease Agents Associated with Pulmonary Alterations in Aborted Bovine Fetuses

SIMPLE SUMMARY: A retrospective study was performed to identify pulmonary alterations and/or pneumonia in aborted bovine fetuses (n = 37) and to associate the presence of infectious disease antigens and nucleic agents with patterns and/or alterations of pulmonary disease. Immunohistochemical (IHC) assays were performed to identify antigens of selected agents associated with bovine respiratory disease: bovine alphaherpesvirus 1 (BoAHV1), bovine viral diarrhea virus (BVDV), bovine parainfluenza virus 3 (BPIV-3), bovine respiratory syncytial virus (BRSV), and Mycoplasma bovis. Molecular assays were performed to identify nucleic acids of a panel of reproductive disease agents and bovine gammaherpesvirus 6 (BoGHV6) in the lungs of 12 fetuses. Only interstitial pneumonia (12/37) and suppurative bronchopneumonia (1/37) were observed; pneumonia was not observed in 65% of the tissues evaluated. The most frequent intralesional agents were BRSV (16.2%; 6/37), BVDV (13.5%; 5/37), and BoAHV1 (8.1%; 3/37). Interstitial pneumonia was associated with BRSV (n = 3), BoAHV1 (n = 3), and BVDV (n = 2); suppurative bronchopneumonia contained a Gram-positive bacterium and intralesional antigens of BVDV and BRSV. Nucleic acid detection identified at least one disease agent in 75% of the fetuses examined. Reproductive pathogens detected included Leptospira spp., (n = 3), BVDV, Neospora caninum, and Brucella abortus (n = 2). BoGHV6 DNA was identified in the lungs of two fetuses with interstitial pneumonia. Single (n = 7), dual (n = 3), triple (n = 4), and quadruple (n = 1) mixed infections were identified while infectious agents were not identified in 59.5% (22/37) of the examined lungs. Single fetal infections were associated with BRSV, BVDV (n = 2), Leptospira spp., BoAHV1, and BoGHV6 (n = 1). These results indicate that the fetuses suffered intrauterine infection through transplacental transmission. The identification of BRSV and BoGHV6 in multiple fetuses with associated pulmonary alterations warrants further investigation relative to the role of these agents in fetopathy and possible direct and/or indirect effects on fetal survival. ABSTRACT: This study investigated the occurrence of selected pathogens of bovine respiratory disease in fetal pulmonary tissue of cattle and associated these with patterns of disease. Fetal pulmonary (n = 37) tissues were evaluated by histopathology; immunohistochemical assays identified intralesional antigens of bovine alphaherpesvirus 1 (BoAHV1), bovine viral diarrhea virus (BVDV), bovine parainfluenza virus 3 (BPIV-3), bovine respiratory syncytial virus (BRSV), and Mycoplasma bovis. Molecular assays were performed to amplify reproductive disease pathogens and bovine gammaherpesvirus 6 (BoGHV6) from 12 lungs. The 2 patterns of pulmonary diseases were interstitial pneumonia (12/37) and suppurative bronchopneumonia (1/37). The frequency of the intralesional antigens identified was BRSV (16.2%; 6/37), BVDV (13.5%; 5/37), BoAHV1 (8.1%; 3/37), M. bovis (5.4%; 2/37), and BPIV-3 (2.7%; 1/37). Interstitial pneumonia was associated with BRSV (n = 3), BoAHV1 (n = 3), and BVDV (n = 2); suppurative bronchopneumonia contained a Gram-positive bacterium and BVDV and BRSV. Reproductive pathogens detected included Leptospira spp., (n = 3), BVDV, Neospora caninum, and Brucella abortus (n = 2). BoGHV6 DNA was identified in the lungs of two fetuses with interstitial pneumonia. These findings suggest that these fetuses were infected transplacentally by several pathogens. The role of some of these pathogens herein identified must be further elucidated in the possible participation of fetal disease.